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EC number: 401-610-3 | CAS number: 122012-52-6 GENIPLEX A
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 1984
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- in accordance with the Commission of European Communities directive 92/69/EEC
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- not specified
- Vehicle:
- no
- Remarks:
- medium was used
- Test organisms (species):
- Scenedesmus sp.
- Details on test organisms:
- TEST ORGANISM
- Strain: Scenedesmus subspicatus - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 24 °C
- pH:
- The pH values of the control and test cultures were observed to increase from pH 8.0 - 8.1 at 0 hours to pH 8.6 - 10.3 at 72 hours. (The increase in pH observed over the 72-hour study period followed a concentration dependant pattern with the lower test material concentrations exhibiting a greater increase in pH)
- Nominal and measured concentrations:
- Nominal: 0.625, 1.25, 2.5, 5.0, 10.0 mg/L
- Details on test conditions:
- - three replicate flasks per concentration
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: spectrophotometer, daily
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0, 0.625, 1.25, 2.5, 5.0, 10 mg/L - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.625 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Observation of abnormalities (for algal test): none stated
- Unusual cell shape: none stated
- Colour differences: none stated
- Flocculation: none stated
- Adherence to test vessels: none stated
- Aggregation of algal cells: none stated - Results with reference substance (positive control):
- not required
- Reported statistics and error estimates:
- students test
- Validity criteria fulfilled:
- yes
- Conclusions:
- The study was performed according to OECD TG 201 with no relevant deviations, the results were so obtained via a scientifically reasonable method. The validity criteria for the study as given by the OECD Guideline 201 were met and the study is therefore considered to be valid. Hence, there is no doubt that the obtained results are not reliable: The toxic effect was investigated by determination of the inhibition of the growth rate of the algae and the yield during the exposure period of 72 hours. The effect of the test item on the growth of Scenedesmus subspicatus has been investigated over a 72-hour period and gave an EbC50 value of 2.7 mg/Land an EfCS0 (24 - 48 h) value of 6.5 mg/L. The No Observed Effect Concentration at 72 hours was 0.625 mg/L.
Based on these results, the test item need to be classified as chronic toxic (3) to the aquatic environment. - Executive summary:
In a 72 hour acute toxicity study, the cultures of algae Scenedesmus subspicatus were exposed to the test item at nominal concentrations of 0, 0.625, 1.25, 2.5, 5.0, 10.0 mg test item/L under static in accordance with the OCED 201 guideline.
All test and control cultures were inspected microscopically at 72 hours. There were no abnormalities detected in any of the control or test cultures at 0.625, 1.25, 2.5 and 5.0 mg/L. However at the test concentration of 10 mg/L the algal cells were observed to be clumped together.
None of the following abnormalities were noted: cell shape, color differences, flocculation, adherence of algae to test vessels, aggregation of algal cell, precipitation in the test solution, or stimulation.
This toxicity study is classified as acceptable and satisfies the guideline requirements for an algae toxicity study.
Based on these results, the test item need to be classified as chronic toxic (3) to the aquatic environment.
Results Synopsis
Test Organism: Algae Scenedesmus subspicatus
Test Type: Static
72 hr EC50: 1.6 mg a.i./L
24-48 hr EC50: 4.3 mg a.i./L
72 hr NOEC: 0.625.mg a.i./L
Reference
Cell concentration:
Mean cell density of control at 0 h | 3.08x104 cells/mL |
Mean cell density of control at 72 h | 9.03x10s cells/mL |
Mean cell density of solvent control at 0 h | 3.08x104 cells/mL |
Mean cell density of solvent control at 72 h | 9.92x10s cells/mL |
show that the cell concentration of the control cultures increased by a factor of 29 and the solvent control cultures by a factor of 32 during the test in line with the OECD Guideline that states the enhancement must be at least by a factor of 16 after 72 hours.
Description of key information
Acute toxicity to algae: NOEC (72h) = 0.625 mg/L, LC50 (72h) = 1.6 mg/L for Scenedesmus subspicatus (OECD 201, GLP, static)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1.6 mg/L
- EC10 or NOEC for freshwater algae:
- 0.625 mg/L
Additional information
Based on these results, the test item need to be classified as chronic toxic (2) to the aquatic environment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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