2-propen-1-amine, N-2-propen-1-yl-, hydrochloride

Skin sensitisation

Administrative data

Endpoint:

skin sensitisation: in vitro

Type of information:

experimental study

Adequacy of study:

key study

Study period:

January 2021 to June 2021

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2021

Report date:

2021

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 442C (In Chemico Skin Sensitisation: Direct Peptide Reactivity Assay (DPRA))

GLP compliance:

yes (incl. QA statement)

Type of study:

other: Direct Peptide Reactivity Assay

Test material

Test material information

Constituent 1

Reference substance name:

bis(prop-2-en-1-yl)amine hydrochloride

EC Number:

612-154-1

Cas Number:

6147-66-6

Molecular formula:

C6H11N.HCl

IUPAC Name:

bis(prop-2-en-1-yl)amine hydrochloride

Test material form:

liquid

Remarks:

Salt dissolved in water

Specific details on test material used for the study:

66% purity
Aqueous solution

In vitro test system

Details on the study design:

Test items were incubated for 24 h +/- 2 h at 25 oC in solution at 100 mM in combination with either cysteine or lysine containing peptides and then run on an HPLC system (20 minute run time) using gradient elution and UV detection at 220 nm to measure peptide concentration.

Test items were compared to reference controls containing the test item solvent in combination with either cysteine or lysine peptide in order to determine the relative percent peptide depletion. Relative percent peptide depletion values were used in a prediction model that assigns test items to one of four reactive classes.

Acceptance Criteria

-Standard curve must have an r2 value >0.99
-The mean percent depletion value of the three replicates for the positive control cinnamic aldehyde should be between 60.8% and 100% for the cysteine peptide and between 40.2% ad 69.4% for the lysine peptide.
-The standard deviation for the positive control replicates should be <14.9% for the percent cysteine depletion and <11.6% for the percent lysine depletion
-Mean peptide concentration of reference controls A should be 0.5 +/- 0.05 mM
-The coefficient of variation (CV) of peptide peak areas for the 9 reference controls B and C should be <15%.

The standard curve is used to assign concentrations to test items and controls.
The RefA controls are peptide plus acetonitrile, prepared to endure peptide concentration is within specified limits.
RefB controls are peptide plus acetonitrile, prepared to ensure peptide stability throughout the run is within the specified limits.
RefC controls are peptide plus acetonitrile and test item solvent, prepared as a control to assess percent peptide depletion.

Using the cysteine 1:10/lysine 1:50 prediction model to assess reactivity classes

0% < mean % depletion <6.38% - No or minimal reactivity - DPRA prediction is negative
6.38% < mean % depletion <22.62% - low reactivity - DPRA prediction is positive
22.62% < mean % depletion <42.47% - moderate reactivity - DPRA prediction is positive
42.47% < mean % depletion <100% - high reactivity - DPRA prediction is positive

Vehicle / solvent control:

water

Positive control:

cinnamic aldehyde [442D]

Results and discussion

Positive control results:

Positive control had a cysteine depletion of 75.031% and is within the acceptance criteria

Positive control had a lysine depletion of 61.90% and is within the acceptance criteria

In vitro / in chemico

Results

Key result

Group:

test chemical

Run / experiment:

mean

Parameter:

other: Mean% Peptide Depletion (Cys + Lys)

Value:

1.366 %

Vehicle controls validity:

valid

Positive controls validity:

valid

Outcome of the prediction model:

negative [in vitro/in chemico]

Other effects / acceptance of results:

All acceptance criteria met

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

2-propen-1-amine, N-2-propen-1-yl-, hydrochloride did not meet the criteria for classification as a skin sensitiser.

Executive summary:

The skin sensitisation potential of 2-propen-1-amine, N-2-propen-1-yl-, hydrochloride was assessed using an in vitro direct peptide reactivity assay according to OECD guidelines 442C during a GLP compliant study.

After 24 hours of the test item being incubated with either cysteine or lysine they were run on a HPLC system and assessed according to the Cysteine 1:10/Lysine 1:50 predicition mode.

Acceptance criteria for the positive control (cinnamic aldehyde) were met.

The test item showed no or minial reactivity and the DPRA prediction was negative.  All acceptance criteria were all met.

2-propen-1-amine, N-2-propen-1-yl, hydrochloride was classified as negative as per the prediction model.