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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Jul 28 - Nov 10, 2020
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
At the start of the exposure and at the renewal (0 and 24 h), samples of the freshly prepared test item concentrations were taken and analyzed.
At renewal and at the end of the exposure (24 and 48 h), sampled of the 24 h old media were taken directly fron the test vessels and analyzed.
Vehicle:
yes
Remarks:
dilution water
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna STRAUS (Clone 5)
- Source: Institut für Wasser-, Boden und Lufthygiene (WaBoLu), Berlin, Germany
- Age: < 24 h

FEEDING DURING TEST
- Food type: 5 x per week ad libitum with a mix of unicellular green algae
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
250 mg CaCO3/L
Test temperature:
20.1 to 20.5 °C
pH:
7.8 to 8.0
Dissolved oxygen:
8.5 to 8.8 mg/L
Conductivity:
330 to 510 lux
Nominal and measured concentrations:
The following dilutions of a filtrate of 100 mg/L were tested: 1:100, 1:220, 1:484, 1:1065 and 1:2343. and a control, corresponding to following time weighted average concentrations of the test item:
60.6, 25.4, 10.6, 4.88 and 3.15 µg test item/L, and a control.

Details on test conditions:
Test vessels:
Glass beakers of 100 mL volume containing approximately 60 mL of test medium. The test vessels were covered with a lid to reduce the loss of water due to evaporation and to avoid the entry of dust into the solutions.

Number of daphnids and replicates: This study encompassed 6 treatment groups (5 dose rates of the test item and a control) each containing 20 individuals.
Application: The test item is not well soluble in test medium. To avoid physical effects of undissolved test item on the daphnids, no concentrations above the solubility limit of the test item in test water was tested. Therefore, a supersaturated stock solution of nominal 100 mg test item/L was prepared by suspending 88.2 and 100.7 mg test item in 882 and 1007 mL test water. The stock suspension was stirred for 6 hours at room temperature in the dark to dissolve as much test item as possible. Then, non-dissolved fractions of the test item were separated from the test medium by membrane filtration (0.45 µm cellulose acetate filter). The solution with dissolved test item was used to prepare the dilutions of 1:100, 1:220, 1:484, 1:1065 and 1:2343.
The test media were prepared just before introduction of the daphnids (= start of the test) and test medium renewal on Day 1.
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.016 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
After 48 hours of exposure no immobilisation of the test animals was observed in the control and up to and including the test item concentration of 10.6 µg test item/L. At the concentrations of 25.4 and 60.6 µg test item/L all animals were immobile
Results with reference substance (positive control):
EC50 (48 h) = 0.986 mg/L
valid range: 0.6 - 2.1 mg/L, acc. to OECD 202

Influence of the test material on the Mobility of Daphnia magna and Sublethal Effects


Time Weighted Average Concentration
[µg test item/L]
 No. of Daphnia tested
 No of immobilised Daphnia after
 % of immobilised Daphnia after
 Observations after
24 h
 48 h
 24 h
 48 h
 24 h
 48 h
Control
 5
 0
 0
 0 0
 0
 0
5
 0
 0
 0
 0
5
 0
 0
 0
 0
5
 0
 0
 0
 0
3.15
 5
 0
 0
 0
 0
 0
 0
5
 0
 0
 0
 0
5
 0
 0
 0
 0
5
 0
 0
 0
 0
4.88
 5
 0
 0
 0
 0
 0
 0
5
 0
 0
 0
 0
5
 0
 0
 0
 0
5
 0
 0
 0
 0
10.6
 5
 0
 0
 0
 0
 0
 0
5
 0
 0
 0
 0
5
 0
 0
 0
 0
5
 0
 0
 0
 0
25.4
 5
 3
 5
 65
 100
 0
 0
5
 4
 5
 0
 0
5
 3
 5
 0
 0
5
 3
 5
 0
 0
60.6
 5
 5
 5
 100 100
 0
 0
5
 5
 5
 0
 0
5
 5
 5
 0
 0
5
 5
 5
 0
 0





Validity criteria fulfilled:
yes
Conclusions:
The toxic effect of the test item to Daphnia magna was assessed in a semi-static concentration-response test.

48-hour NOEC: 10.6 µg test item/L.
48-hour LOEC: 25.4 µg test item/L
48-hour EC50: 16.0 µg test item/L.

The initial concentrations and the maintenance of the exposure concentrations during the test were verified in the analytical part. All reported results refer to time weighted average concentrations, since the test item concentrations were not within ± 20 % of the measured initial concentrations during the test.

Description of key information

The toxic effect of the test item to Daphnia magna was assessed in a semi-static concentration-response test. The 48-hour NOEC was determined to be 10.6 µg test item/L. The 48-hour LOEC was determined to be 25.4 µg test item/L and the 48-hour EC50 value was calculated to be 16.0 µg test item/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
16 µg/L

Additional information