Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2020-03-27 to 2020-06-10
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
- Report date:
- 2020
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- other: OECD. OECD series on testing and assessment number 23 (Second Edition), Guidance Document on Aqueous-Phase Aquatic Toxicity Testing of Difficult Test Chemicals. Paris: OECD, Adopted on February 8, 2019.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The actual concentration of test substance in the 100 mg/L WAF test group were measured at 0 h and 72 h in the new solutions and measured at 24 h and 96 h in the old solutions by GC. The blank control was measured at 0 h in the new solution and 24 h in the old solution.
100 mL test solution was transfened into a separating funnel and be extracted three times with 20 mL, 15 mL and 10 mL of n-hexane, respectively. The extract solution was collected into 50 mL volume flask and diluted to 50 mL with n-hexane, the extract was filtered through 0.45 µm aqueous phase nylon filter membranes (the first 5 mL - 10 mL filtrate was discarded) before analysis. The samples was analyzed immediately, otherwise the samples were stored at 2 °C - 8 °C and protected from light after pretreatment, and the measurement was carried out within 96 h.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- 1.000 g of test substance was weighed on a piece of watch glass, then it was slowly put into a 10 L aspirator bottle which contained 10000 mL dechlorinated tap water, and the nominal loading rate of 100 mg/L test suspension was obtained. The suspension was continuously stirred with a magnetic stirrer for 48 h, and the stirring intensity was sufficient to maintain a vortex height of about 10% of liquid depth.
After the completion of stirring, the suspension was allowed to stand for 2 hour. Then the middle solution was collected through the outfall at the bottom of aspirator bottle (the first 100 - 200 mL solution was discarded), and the nominal loading rate of 100 mg/L WAF solution was obtained and used as test solution.
The preparation of test solution is in according with the OECD guidance document NO.23 (2019).
Test organisms
- Test organisms (species):
- other: Rare Minnow (Gobiocypris rarus)
- Details on test organisms:
- Rare Minnow (Gobiocypris rarus) with the batch number was FGr20191106-1115.
Before the start of the test, Rare Minnow (Gobiocypris rarus) were acclimated for 55 days under the same conditions of the test (water quality: dechlorinated tap water, temperature: 23 °C ± 2 °C, photoperiod: 14 h light / 10 h darkness). The dissolved oxygen concentration during acclimation was kept above 80% of the air saturation value. The fish were fed one time on each workday until 24 hours before the test was started.
The mortality was 0% during 7 days prior to the start of the test, which met the requirement of mortalities being less than 5% of the population.
Study design
- Test type:
- semi-static
- Water media type:
- other: dechlorinated tap water
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 110 mg/L (expressed as CaCO3)
- Test temperature:
- 21.1 °C to 23.5 °C
- pH:
- 7.80 to 8.34
- Dissolved oxygen:
- 83% to 102%
- Nominal and measured concentrations:
- nominal: 100 mg/L WAF
measured: lower than LOD of 0.125 mg/L - Details on test conditions:
- Photoperiod: 14 h light / 10 h darkness
Feeding: No feedding
Aeration: no aeration - Reference substance (positive control):
- yes
- Remarks:
- K2Cr2O7
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: WAF (water accommodated fraction)
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LL100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: WAF (water accommodated fraction)
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LL0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: WAF (water accomodated fraction)
- Basis for effect:
- mortality (fish)
- Results with reference substance (positive control):
- The sensitivity test of reference substance K2Cr2O7 showed that the 96 h-LC50 of K2Cr2O7 to Rare Minnow (Gobiocypris rarus) was 215 mg/L and the 95% confidence limits ranged from 171 mg/L to 270 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- At the end of the test, the cumulative mortality of the blank control was 0%, which met the validity criterion that the mortality should not exceed 10%.
- Conclusions:
- The acute toxicity of test substance to Rare Minnow (Gobiocypris rarus) was determined in a limit test under semi-static conditions (24 h intervals). At the end of the test, the cumulative mortality in the 100 mg/L WAF test group was 0%. Hence, the 96 h - LL50 of test substance for Rare Minnow (Gobiocypris rarus) was greater than 100 mg/L (Based on the nominal loading rate WAF).
- Executive summary:
The purpose of the study is to evaluate the possible toxicity of the test substance to Rare Minnow (Gobiocypris rarus) for a period of 96 h.
According to the results of preliminary tests, a nominal loading rate of 100 mg/L of test substance water accommodated fraction (WAF) test group and a blank control were selected.
The preparation of test solution is in according with the OECD guidance document NO.23 (2019).
The actual concentrations of test substance in the 100 mg/L WAF test group were measured at 0 h and 72 h in the new solutions and measured at 24 h and 96 h in the old solutions by Gas Chromatography (GC). The measured concentrations of test substance in blank control were both lower than LOD of 0.125 mg/L at 0h (new solution) and 24 h ( old solution).
At 0h, 72 h (new solutions) and 24 h, 96 h (old solutions), the measured concentrations of test substance in 100 mg/L WAF test group were all lower than LOD of 0.125 mg/L. The measured concentration lower than the LOD of the analytical method, which indicated that the test substance is poorly soluble in test water, but this did not necessarily mean that there was no test item present in the test solution.
The acute toxicity of test substance to Rare Minnow (Gobiocypris rarus) was detennined in a limit test under semi-static conditions (24 h intervals). At the end of the test, the cumulative mortality in the 100 mg/L WAF test group was 0%. Hence, the results for the 96 h median lethal loading rate (LL50), the lowest loading rate causing 100% mortality (LL100) , and the highest loading rate causing 0% mortality (LL0) were described based on the nominal loading rate of the test substance.
LL50 > 100 mg/L (Based on the nominal loading rate WAF)
LL100 > 100 mg/L (Based on the nominal loading rate WAF)
LL0 = 100 mg/L (Based on the nominal loading rate WAF)
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Nors ECHA internete skelbia daug informacijos jūsų kalba, dalis informacijos šiame puslapyje pateikiama tik anglų kalba. Daugiau apie ECHA daugiakalbystės politiką.
Sveiki atvykę į ECHA svetainę! Ši tinklavietė nepritaikyta naudoti su naršykle Internet Explorer 7 (ir ankstesnėmis jos versijomis). Jums reikalinga naujesnė naršyklės Internet Explorer versija.
Siekdami užtikrinti, kad būtų patogu naudotis mūsų svetaine, jos tinklapiuose naudojame slapukus.
Daugiau informacijos apie tai, kaip naudojame slapukus.