Magnesium octadecylbenzenesulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

21 July 2014-08 August 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

Qualifier:

according to guideline

Guideline:

ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 1: Static Method)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14, 2000.

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Samples for analysis were taken from the limit concentration and the control according to the schedule below. In addition, the filter used to prepare the limit concentration was retained for possible analysis of the residue.
Frequency: at t=0 h, t=24 h and t=96 h
Volume: 2.0 ml
Storage: Samples were stored in a freezer until analysis.

Vehicle:

no

Details on test solutions:

The batch of EXP1313100 tested was a brown solid that was not completely soluble in test medium at the loading rates initially prepared.

All test solutions were prepared separately. Various loading rates of the test substance were added to the test medium applying two days of magnetic stirring in order to reach maximum solubility. The resulting aqueous mixtures were filtered through a 0.45 µm membrane filter (Whatman) where after the clear and colourless Water Accommodated Fractions (WAFs) were used for testing.

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Species: Carp (Cyprinus carpio, Teleostei, Cyprinidae) Linnaeus, 1758
Source: Zodiac, proefacc, "De Haar Vissen", Wageningen University and Research Centre, The Netherlands.
Medium: Adjusted ISO medium, formulated using RO-water (tap-water purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands)

Mean length Range-finding test: 3.1 ± 0.3 cm
Final test: 3.3 ± 0.3 cm
Mean weight1 Range-finding test: 0.82 ± 0.26 g
Final test: 1.11 ± 0.22 g
Characteristics: F1 from a single parent-pair bred in UV-treated water.
Reason for selection: This system has been selected as an internationally accepted species.
ACCLIMATION
- Acclimation period: At least 12 days after delivery
- Acclimation conditions (same as test or not): same as the test
- Feeding: Daily with pelleted fish food
- Health during acclimation (any mortality observed): In the batch of fish used for the test, mortality during the seven days prior to the start of the test was less than 5%.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

180 mg/l expressed as CaCO3

Test temperature:

between 22 °C and 23 °C

pH:

between 7.5 and 8.1

Dissolved oxygen:

between 5.8 and 8.8 mg/l

Nominal and measured concentrations:

Nominal concentrations:Control and a WAF prepared at a loading rate of 100 mg/L.
Measured concentration: Analysis of the samples taken at the start of the limit test showed a measured concentration of 0.0045 mg/L in the WAF prepared at a loading rate of 100 mg/L. This concentration slightly decreased to 72% of initial at the end of the test. The average exposure concentration was calculated to be 0.0038 mg/L.

Details on test conditions:

Test type: Static
Test vessels: 20 litres, all-glass, containing 20 litres of test solution
Test medium: Adjusted ISO medium with a hardness of 180 mg CaCO3 per litre and a pH of 7.7 ± 0.3
Number of fish: 7 per concentration
Loading: 0.39 g fish/litre, i.e. 7 fish per 20 litres of test medium
Illumination: 16 hours photoperiod daily
Aeration: Aeration was introduced after 1 day of exposure.
Feeding: No feeding from 24 hours prior to the test and during the total test period

TEST MEDIUM / WATER PARAMETERS
Medium: Adjusted ISO medium, formulated using RO-water (tap-water purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands) with the following composition:
CaCl2.2H2O: 211.5 mg/L
MgSO4.7H2O: 88.8 mg/L
NaHCO3: 46.7 mg/L
KCl : 4.2 mg/L


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours photoperiod daily

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :LC50 t=24, 48, 72, 96H

TEST CONCENTRATIONS
- Final study: control and a WAF prepared at a loading rate of 100 mg/L

Reference substance (positive control):

yes

Remarks:

pentachlorophenol (PCP)

Duration:

24 h

Dose descriptor:

LL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 0.004 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 0.004 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 0.004 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.004 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Behavioral abnormalities:
- Mortality of control: No mortality in control
- Abnormal responses: no abnormalities were observed
- Effect concentrations exceeding solubility of substance in test medium: yes

Results with reference substance (positive control):

Under the conditions of the present test PCP induced no lethal effects in carp at 0.10 mg/L. The
96h-LC50 for carp exposed to PCP was 0.24 mg/L. This effect was already reached within 48 hours of exposure.

Reported statistics and error estimates:

Calculation of LC50
The 24, 48, 72 and 96h-EC50 values could not be determined because the observed effects were below 50%.

Validity criteria fulfilled:

yes

Conclusions:

No mortality was recorded at any of the loading rates of EXP1313100 tested.
The 96h-LL*50 was above a loading rate of 100 mg EXP1313100 per litre. The concentration of EXP1313100 in the WAF prepared at a loading rate of 100 mg/L was determined to be 0.0038 mg/L based on the average exposure concentration.

*LL = lethal loading rate

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

21 July 2014-08 August 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

Qualifier:

according to guideline

Guideline:

ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 1: Static Method)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14, 2000.

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Samples for analysis were taken from the limit concentration and the control according to the schedule below. In addition, the filter used to prepare the limit concentration was retained for possible analysis of the residue.
Frequency: at t=0 h, t=24 h and t=96 h
Volume: 2.0 ml
Storage: Samples were stored in a freezer until analysis.

Vehicle:

no

Details on test solutions:

The batch of EXP1313100 tested was a brown solid that was not completely soluble in test medium at the loading rates initially prepared.

All test solutions were prepared separately. Various loading rates of the test substance were added to the test medium applying two days of magnetic stirring in order to reach maximum solubility. The resulting aqueous mixtures were filtered through a 0.45 µm membrane filter (Whatman) where after the clear and colourless Water Accommodated Fractions (WAFs) were used for testing.

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Species: Carp (Cyprinus carpio, Teleostei, Cyprinidae) Linnaeus, 1758
Source: Zodiac, proefacc, "De Haar Vissen", Wageningen University and Research Centre, The Netherlands.
Medium: Adjusted ISO medium, formulated using RO-water (tap-water purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands)

Mean length Range-finding test: 3.1 ± 0.3 cm
Final test: 3.3 ± 0.3 cm
Mean weight1 Range-finding test: 0.82 ± 0.26 g
Final test: 1.11 ± 0.22 g
Characteristics: F1 from a single parent-pair bred in UV-treated water.
Reason for selection: This system has been selected as an internationally accepted species.
ACCLIMATION
- Acclimation period: At least 12 days after delivery
- Acclimation conditions (same as test or not): same as the test
- Feeding: Daily with pelleted fish food
- Health during acclimation (any mortality observed): In the batch of fish used for the test, mortality during the seven days prior to the start of the test was less than 5%.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

180 mg/l expressed as CaCO3

Test temperature:

between 22 °C and 23 °C

pH:

between 7.5 and 8.1

Dissolved oxygen:

between 5.8 and 8.8 mg/l

Nominal and measured concentrations:

Nominal concentrations:Control and a WAF prepared at a loading rate of 100 mg/L.
Measured concentration: Analysis of the samples taken at the start of the limit test showed a measured concentration of 0.0045 mg/L in the WAF prepared at a loading rate of 100 mg/L. This concentration slightly decreased to 72% of initial at the end of the test. The average exposure concentration was calculated to be 0.0038 mg/L.

Details on test conditions:

Test type: Static
Test vessels: 20 litres, all-glass, containing 20 litres of test solution
Test medium: Adjusted ISO medium with a hardness of 180 mg CaCO3 per litre and a pH of 7.7 ± 0.3
Number of fish: 7 per concentration
Loading: 0.39 g fish/litre, i.e. 7 fish per 20 litres of test medium
Illumination: 16 hours photoperiod daily
Aeration: Aeration was introduced after 1 day of exposure.
Feeding: No feeding from 24 hours prior to the test and during the total test period

TEST MEDIUM / WATER PARAMETERS
Medium: Adjusted ISO medium, formulated using RO-water (tap-water purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands) with the following composition:
CaCl2.2H2O: 211.5 mg/L
MgSO4.7H2O: 88.8 mg/L
NaHCO3: 46.7 mg/L
KCl : 4.2 mg/L


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours photoperiod daily

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :LC50 t=24, 48, 72, 96H

TEST CONCENTRATIONS
- Final study: control and a WAF prepared at a loading rate of 100 mg/L

Reference substance (positive control):

yes

Remarks:

pentachlorophenol (PCP)

Duration:

24 h

Dose descriptor:

LL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 0.004 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 0.004 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 0.004 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.004 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Behavioral abnormalities:
- Mortality of control: No mortality in control
- Abnormal responses: no abnormalities were observed
- Effect concentrations exceeding solubility of substance in test medium: yes

Results with reference substance (positive control):

Under the conditions of the present test PCP induced no lethal effects in carp at 0.10 mg/L. The
96h-LC50 for carp exposed to PCP was 0.24 mg/L. This effect was already reached within 48 hours of exposure.

Reported statistics and error estimates:

Calculation of LC50
The 24, 48, 72 and 96h-EC50 values could not be determined because the observed effects were below 50%.

Validity criteria fulfilled:

yes

Conclusions:

No mortality was recorded at any of the loading rates of EXP1313100 tested.
The 96h-LL*50 was above a loading rate of 100 mg EXP1313100 per litre. The concentration of EXP1313100 in the WAF prepared at a loading rate of 100 mg/L was determined to be 0.0038 mg/L based on the average exposure concentration.

*LL = lethal loading rate

Description of key information

No mortality was recorded at any of the loading rates. The 96h-LL50 was above a loading rate of 100 mg/l.