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Diss Factsheets
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EC number: 948-066-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vitro
- Study period:
- 2019
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- the study does not need to be conducted because the substance is classified as skin corrosion (Category 1, 1A, 1B or 1C)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD guideline No. 442E: “In vitro skin sensitization assays addressing the Key Event on activation of dendritic cells on the adverse outcome pathway for Skin sensitization”, 25 June 2018
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- activation of dendritic cells
Test material
- Reference substance name:
- Ethanol, 2-(2-butoxyethoxy)-, reaction products with phosphorus oxide (P2O5), compds. with N,N-dimethylcyclohexanamine
- EC Number:
- 948-066-8
- Molecular formula:
- C16H36NO6P C24H52NO8P C24H53NO11P2 C32H68NO10P C8H17N H3O4P
- IUPAC Name:
- Ethanol, 2-(2-butoxyethoxy)-, reaction products with phosphorus oxide (P2O5), compds. with N,N-dimethylcyclohexanamine
- Test material form:
- liquid: viscous
Constituent 1
- Specific details on test material used for the study:
- Batch No.: 0002293298
Description: Viscous colourless to yellowish liquid
Storage conditions: At room temperature
Specific requirements (handling conditions): None
Purity: Considered as 100%
Correction factor: No correction factor
In vitro test system
- Details on the study design:
- TEST SYSTEM
1/ Cell line
The THP-1 is an immortalized human monocytic leukemia cell line derived from an acute monocytic leukemia patient. The THP-1 cell line is obtained from ATCC (Ref: TIB-202, American Type Culture Collection, Manassas, USA) by the intermediate of LGC Standards (Molsheim, France).
The THP-1 cells are stored in a cryoprotective medium in a liquid nitrogen container. Cells will be grown using general culture procedures. They will be cultured in cRPMI medium and maintained in a humidified incubator set at 37°C, 5% CO2 and will not be allowed to exceed a cell density of 1 x 106 cells/mL or more than 30 passages.
The culture medium (cRPMI) will be composed of RPMI 1640 with 10% foetal bovine serum (FBS), 0.05 mM 2-mercaptoethanol and with penicillin (100 units/mL) and streptomycin (100 μg/mL).
During cell culturing, cell viability will be checked using trypan blue.
2/ Cell culture for testing
For testing, THP-1 cells are seeded at a density between 0.1 x 106 cells/mL and 0.2 x 106 cells/mL, and pre-cultured in culture flasks for a maximum of 72 hours. Cell will not let to exceed density of 1 x 106 cells/mL. On the day of testing, cells harvested from culture flasks are re-suspended with fresh culture medium at 2 x 106 cells/mL. Then, 500 μL of cells suspension are distributed into a 24-well flat-bottom plate (i.e. 1 x 106 cells/well).
3/ Reactivity check
Two weeks after thawing, a reactivity check is performed to qualify the cells of each working cell bank before testing. A reactivity check assay is performed by testing cell response after contact
with Lactic Acid (≥85% purity), DNCB (≥99% purity) and NiSO4 (≥99% purity) under an internal Citoxlab reference number. Results from reactivity check tests are compiled in Citoxlab France internal data, with negative and positive control data obtained during each test.
Results and discussion
In vitro / in chemico
Results
- Key result
- Remarks on result:
- other: the test is currently ongoing.
Applicant's summary and conclusion
- Interpretation of results:
- other: the test is currently ongoing.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.