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EC number: 946-448-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2013-12-16 until 2014-01-15
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all (At the end of the exposure period, the replicates were pooled at each concentration before sampling.)
- Sampling frequency: at t=0h and t=48h
- Sample volume: 2.0 mL
- Sample storage conditions before analysis: stored in a freezer until analysis (<= -15°C) - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
Preparation of test solutions started with a nominal concentration of 100 mg/l for the combined limit/range-finding test and a nominal concentration of 10 mg/l for the final test. Fifteen to twenty minutes of magnetic stirring was applied to accelerate the dissolving of the test substance in the test medium. The lower test concentrations were prepared by subsequent dilutions of the 10 and 100 mg/l concentrations in test medium. Test solutions up to and including 1.0 mg/l were clear and colourless, while higher test concentrations were increasingly hazy with increasing concentration. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain: Daphnia magna Straus
- Source: WIL Research Europe B.V., In-house laboratory culture
- Age at study initiation: < 24 h
- Method of breeding: acyclic parthenogenesis
- Feeding during test: no
BREEDING
- Maximum age of the cultures: 4 weeks
- Breeding conditions: M7 medium, 18-22°C
- Renewal of cultures: after 7 days of cultivation half of the medium twice a week
- Type of food: suspension of freshwater algae
- Feeding frequency: daily
- Validity of batch: immobilisation < 20%; no presence of males, ephippia or discoloured animals; no delay in first brood - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 180 mg CaCO3/L (Elendt M7 medium)
- Test temperature:
- 19.1 - 19.6°C
- pH:
- 7.8 - 8.2
- Dissolved oxygen:
- 9.3 - 9.5 mg/L
- Nominal and measured concentrations:
- Actual test concentrations were in agreement with nominal (108-117%). The lowest test concentration slightly decreased to 79% of initial at the end of the test but was still in agreement with nominal (86%). All higher test concentrations remained stable (92-98% of nominal). Given these results, the EC50 was based on the nominal test concentrations.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL, all-glass, containing 80 mL of test solution
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): not applicable
- Renewal rate of test solution (frequency/flow rate): not applicable
- No. of organisms per vessel: 5 per vessel
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): not applicable
TEST MEDIUM / WATER PARAMETERS
- Medium: Adjusted ISO medium
- Source/preparation of dilution water: tap water purified by Reverse Osmosis (ROwater, GEON Waterbehandeling, Berkel-Enschot, The Netherlands)
- Culture medium different from test medium: yes (Elendt M7 medium)
- Intervals of water quality measurement: at 0 hours and at 48 hours
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours
- Light intensity: 271 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Immobility: at 24 hours and at 48 hours
TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: not applicable
- Test concentrations range-finding test [mg/L]: 0.1, 1.0, 10, 100
- Test concentrations main test [mg/L]: 0.10, 0.18, 0.32, 0.56 and 1.0 mg/l
- Results used to determine the conditions for the definitive study: 100% immobilisation at 1 mg/L, 0% immobililisation at 0.1 mg/L - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.26 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL 0.24-0.28 mg/L
- Details on results:
- - Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Reported statistics and error estimates:
- Parameters of the probit analysis at 48h
--------------------------------------------
Computation runs: 4.0000
Slope b: 14.9147
Intercept a: 8.3118
Variance of b: 5.2737
Goodness of Fit
Chi²: 0.4521
Degrees of freedom: 3.0000
p(Chi²): 0.9293
Log EC50: -0.58186
SE Log EC50: 0.0169
g-Criterion: 0.0911
F: 279.9170
p(F) (df: 1;3): 0.0000 - Validity criteria fulfilled:
- yes
- Conclusions:
- The 48h-EC50 was 0.26 mg/l (95% confidence interval between 0.24 and 0.28 mg/l).
- Executive summary:
Acute Toxicity Study in Daphnia magna with Benzenesulfonic acid, 4-C15-C16-sec-alkyl derivs.
The study procedures described in this report were based on the OECD guideline No. 202, 2004. In addition, the procedures were designed to meet the test methods of the Commission Regulation (EC) No 440/2008, Part C.2, 2008 and the ISO International Standard 6341, 1996.
The batch of Benzenesulfonic acid, mono, 4-C15-C16-sec-alkyl derivs. tested was a dark brown viscous liquid with a purity of 89.2% and completely soluble in test medium at the concentrations tested.
A final test was performed based on the results of a preceding combined limit/range-finding test. Preparation of test solutions started with a nominal concentration of 10 mg/l. Fifteen to twenty minutes of magnetic stirring was applied to accelerate the dissolving of the test substance in the test medium. The lower test concentrations were prepared by subsequent dilutions of the 10 mg/l concentration in test medium. Test solutions up to and including 1.0 mg/l were clear and colourless.
Twenty daphnids per concentration (four replicates, five daphnids per replicate) were exposed to a control and to nominal test concentrations of 0.10, 0.18, 0.32, 0.56 and 1.0 mg/l. The total exposure period was 48 hours and samples for analytical confirmation of actual exposure concentrations were taken at the start and at the end of the test.
Analysis of the samples taken at the start of the test showed that the actual test concentrations were in agreement with nominal (108-117%). The lowest test concentration slightly decreased to 79% of initial at the end of the test but was still in agreement with nominal (86%). All higher test concentrations remained stable (92-98% of nominal). Given these results, the EC50 was based on the nominal test concentrations.
The study met the acceptability criteria prescribed by the protocol and was considered valid.
The 48h-EC50 was 0.26 mg/l (95% confidence interval between 0.24 and 0.28 mg/l).
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP guideline study. No analytical verification of the test concentrations
- Principles of method if other than guideline:
- Method: other: Directive 79/831/EEC, Annex V, Part C
- GLP compliance:
- no
- Analytical monitoring:
- no
- Test organisms (species):
- Daphnia magna
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 62.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- not neutralized
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 108.82 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- not neutralized
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 250 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- not neutralized
- Conclusions:
- The 48 h EC50 of MIPA to Daphnia magna was 108.82.
Referenceopen allclose all
Observations range-finding test:
All daphnids exposed to nominal concentrations of 1.0 mg/l and higher were immobilised after 24 hours of exposure. No immobility was observed at the lowest test concentration of 0.10 mg/l and the control during the test period. The expected EC50 was between nominal concentrations of 0.10 and 1.0 mg/l.
Mobile daphnids
Nominal conc.(mg/l) |
Mobile daphnids after |
||||
0 h |
3 h |
6 h |
24 h |
48 h |
|
0 |
20 |
20 |
20 |
20 |
20 |
3.9 |
20 |
20 |
20 |
20 |
20 |
7.8 |
20 |
20 |
20 |
20 |
20 |
15.6 |
20 |
20 |
20 |
20 |
20 |
31.2 |
20 |
20 |
20 |
20 |
20 |
62.5 |
20 |
20 |
20 |
20 |
20 |
125 |
20 |
20 |
20 |
20 |
6 |
250 |
20 |
20 |
20 |
0 |
0 |
500 |
20 |
1 |
0 |
0 |
0 |
pH values
Nominal conc.(mg/l) |
pH values after |
|
0 h |
48 h |
|
0 |
8.03 |
8.15 |
3.99 |
8.32 |
8.03 |
7.81 |
8.54 |
8.02 |
15.6 |
8.89 |
8.05 |
31.2 |
9.20 |
8.14 |
62.5 |
9.53 |
8.4 |
125 |
9.79 |
8.71 |
250 |
10.04 |
8.96 |
500 |
10.3 |
9.09 |
Description of key information
The short-term toxicity of Benzenesulfonic acid, 4-C15-16-sec-alkyl derivs. was assessed in a static 48h acute study in Daphnia magna according to OECD guideline 202. The result of an 48h-EC50 of 0.26 mg/L (95% CL 0.24 -0.28 mg/L) can be considered reliable without restrictions.
For the second read across substance (MIPA), the 48 hr EC50 to Daphnia magna was 108.82 mg/L
As the short-term toxicity to invertebrate value for Benzenesulfonic acid, 4 -C15 -16 -sec-alkyl derivs. was significantly lower than that for MIPA, it will be used for Benzenesulfonic acid, 4 -C15 -16 -sec-alkyl derivs. MIPA-salt.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.26 mg/L
Additional information
The test concentrations were stable and within the acceptable range during the test. Therefore the estimated 48h-EC50 of 0.26 mg/L (95% CL 0.24 -0.28 mg/L) is based on the nominal test concentrations.
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