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EC number: 831-423-6 | CAS number: 2125692-22-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 7/6/18 - 27/7/18
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Reaction mass of L-valine and ethanesulphonic acid and octadecan-1-ol and docosan-1-ol and eicosan-1-ol
- Cas Number:
- 2125692-22-8
- Molecular formula:
- C25-31H54-66NO5S
- IUPAC Name:
- Reaction mass of L-valine and ethanesulphonic acid and octadecan-1-ol and docosan-1-ol and eicosan-1-ol
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Details on sampling:
- The test was performed with Elendt M4 medium according to OECD 202 (2004).
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- The stock solution was prepared as water saturated solution by adding 400.3 mg test item to 1000 mL test medium and shaking for 24.2 h using an overhead shaker at 22.9 – 23.7 °C in diffuse light. The water saturated solution was filtered through a fibre glass filter with a retaining range till 0.6 μm. The filter was prepared by rinsing with purified water and preconditioning with ca. 50 mL stock solution (which was discarded) to reduce adsorption of the test item. This filtered stock solution was used as highest test item loading rate in the test. The negative control (NC; test medium) was treated in the same way as the test item solution. Four replicates, with five daphnids each, were prepared for each test item treatment and the negative control.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The test organism Daphnia magna Straus originates from the clone breeding of the Federal Environment Agency, department IV 2.4, Berlin, and has been cultivated at Hydrotox GmbH since October 2012. The daphnids are cultured at 20 ± 2 °C with 16 h light : 8 h dark and placed into fresh Elendt M4 medium twice a week. They are kept at approx. 10 animals / 200 mL and fed on workdays with Desmodesmus subspicatus algae corresponding to 0.1 mg C × daphnid-1 × day-1. The test organisms used were 2.5 – 24 h old at the start of the test.
Quality assurance takes place at regular intervals by testing the sensitivity of the test organisms to the reference item potassium dichromate (Sigma, Steinheim, Germany; Lot no.: MKBF2111V). The recent quality testing was performed in April 2018 with EC50 (24h) = 1.8 mg/L (CL 95 %: 1.64 – 2.02 mg/L), which is in the declared range of 0.6 – 2.1 mg/L (24 h) according to OECD 202.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- specified temperature 18 – 22 °C, constant within ± 1 °C
- pH:
- 7.7
- Dissolved oxygen:
- 7.7 mg/L
- Conductivity:
- 701 μS/cm
- Nominal and measured concentrations:
- Nominal test item loading rate [mg/L] * 0 25 50 100 200 400
Test vessels (replicates) 4
Test solution / Test medium [mL] 40 - Details on test conditions:
- The test vessels are kept in an incubator (specified temperature 18 – 22 °C, constant within ± 1 °C) with 16 h light : 8 h dark. The pH as well as the O2-concentrations were measured at the start (0 h) and at the end of the test (48 h) and the temperature was measured continuously.
- Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 63.83 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 19.08 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 34.86 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 34.86 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Reported statistics and error estimates:
- With the statistical software ToxRat Professional 3.2.1 (ToxRat Solutions GmbH, Alsdorf, Germany),
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 (48hr) = 63.83 mg/L
- Executive summary:
Study: Daphnia magna Acute Immobilisation Test according to
OECD 202 (13 April 2004)
Sponsor: INOLEX Inc.
Test item: Brassicyl Valinate Esylate
Chemical name: Brassicyl Valinate Esylate
Molecular formula: Not available
CAS number: 2125692-22-8
Batch/Lot number: HC9430
Purity: 100 %
Test organism: Daphnia magna Straus
Test duration: 48 h
Application of test item: 25 – 400 mg/L nominal
Experimental phase: 12 – 14 June 2018
Study initial / completion date: 07 June 2018 / 27 July 2018
Results:
As the measured test item concentrations are not within ± 20 % of the nominal loading rates,
according to OECD 202 (2004), all results are given in relation to the analytically measured test
item concentrations (geometric mean concentrations).
(Lowest/No Observed) Effect Concentration Measured test item concentration [mg/L]
95 % Confidence Limits (CL) 48 h
EC50 63.83
CL 36.63 – 1401.26
EC10 19.08
CL 7.85 – 30.94
LOEC ˃ 34.86
NOEC ≥ 34.86
The test is valid according to OECD Test Guideline 202 (13 April 2004).
The measured TOC concentrations in the test item treatments were 6.05 – 12.31 % of the nominal TOC concentrations.
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