Reaction mass of barium chromate, copper dichromium tetraoxide and copper oxide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 30 July to 3rd September 2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: compliant to GLP and testing guideline; adequate coherence between data, comments and conclusions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

dioxo-1,3-dioxa-2-chroma-4-baracyclobutane; dioxo-2,4-dioxa-3-cupra-1,5-dichromapentane; oxocopper

EC Number:

701-287-1

Molecular formula:

CuO, CuCr2O4, BaCrO4

IUPAC Name:

dioxo-1,3-dioxa-2-chroma-4-baracyclobutane; dioxo-2,4-dioxa-3-cupra-1,5-dichromapentane; oxocopper

Details on test material:

- Name of test material (as cited in study report): Chromite de cuivre
- Substance type: multiconstituents
- Physical state: black powder
- Composition of test material, percentage of components: 34% of copper, 8.5% of baryum, 31% of chrome
- Purity test date: 11 January 2010
- Lot/batch No.: 09/0177
- Expiration date of the lot/batch: 17 August 2014
- Storage condition of test material: in darkness at room temperature

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Before and after each renewal, two aliquots of 5 mL were taken in each group containing the test item and in the stock solution.
These aliquots were stored at approximately -16°C (deep frozen) and transferred to ICP-AES unit at the end of test to be analysed for chromium and barium contents determination.

Test solutions

Vehicle:

no

Details on test solutions:

TEST SOLUTION PREPARATION
As the test item is not soluble in water, the stock solutions was prepared by stirring an excess amount of the test item (100 mg/L) in test medium for up to 24 hours. Then after a filtration (0.22 µm), the aqueous phase (corresponding to the limit of solubility/saturation under our experimental conditions (LS)) was collected and used as a stock solution.
Filtration on filter 0.22 µm was chosen because the certificate of analysis indicates that 50% of the particles constituting the test item are < 0.43 µm.
Test solutions was prepared by further dilution of the stock solution with test medium. For the second definitive test each test solution was prepared by dilution by tow from the higher test solution.
Test vessels were filled directly from the test solution containers after preparation.
The solutions were renewed after 24 hours of exposure.
The pH range considered as appropriate for a normal life of test organisms is circa 6 to 9.
So the pH of the stock solution was measured. The pH of the stock solution was in this range (8,04).

- Test item stock solution preparation:
For the first definitive test, test item stock solution was prepared as follow :

Date Weight (mg): Volume (with test media) Theoretical concentration
mL (chromite de cuivre) mg/l
Chromite de Cuivre,
Batch 09/0117 August 2nd 2010 40,22 400 100,55
BPL10-0008.001 August 3rd 2010 40,38 400 100,95


For the second definitive test, test item stock solution was prepared as follow :

Date Weight (mg): Volume (with test media) Theoretical concentration
mL: (chromite de cuivre) mg/l
Chromite de Cuivre,
Batch 09/0117 August 24th 2010 51,56 500 103,12
BPL10-0008.001 August 25th 2010 49,92 500 99,84


- Test solutions preparation:
For the first definitive test, test solutions were prepared by further dilutions of the test item stock solution (LS) stirred with test media (LS/80 ; LS/40 ; LS/20 ; LS/10 and LS/5).
For the second definitive test, each test solution was prepared by dilution by tow from the higher test solution stirred with test media (LS/16 ; LS/8 ; LS/4 ; LS/2 and LS).

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISMS
- Source: breeding lab
- Age at study initiation (mean and range, SD): between 6 and 24 hours old
- Length at study initiation (length definition, mean, range and SD): between 560 µm and 800 µm
- Method of breeding: produced from parthenogenically reproducing brood female population
- Feeding during test: no data
- Food type: no data
- Amount: no data
- Frequency: no data

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

second definitive test: total hardness was only measured in the controls at the start and the end of the test.
Total Hardness (°F)
Concentration 0H 48H
Control 21,8 22,3

Test temperature:

first definitive test: 21.7°C to 22°C
second definitive test: 21.8°C to

pH:

first definitive test: from 7.61 to 8.98
second definitive test: from 7.86 to 8.04

Dissolved oxygen:

first definitive test: 8.34 to 8.90 mg 02/L
second definitive test: 86.2 to 99.9 mg 02/L

Salinity:

Not measured

Nominal and measured concentrations:

Concentrations are expressed in chromite de cuivre by calculation with chromium concentration. The chromium concentration is used because it is the lowest test item concentration (with Cr content calculation from CoA); therefore, it is the worst determination for toxicity test.
See section "any other information on materials and methods.

Details on test conditions:

TEST SYSTEM
- Renewal rate of test solution (frequency/flow rate): every 24 hours
- No. of organisms per vessel: 5 Daphnids were introduced in each replicates
- No. of vessels per concentration (replicates): 4 replicates per concentration
- No. of vessels per control (replicates): 4 replicates

OTHER TEST CONDITIONS
- Photoperiod: light/dark cycle = 16/8 hours

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility inhibition

TEST CONCENTRATIONS
- Test concentrations: For the first definitive test, test solution concentrations were LS/80 ; LS/40 ; LS/20 ; LS/10 and LS/5. For the second definitive test, test solution concentrations were LS/16 ; LS/8 ; LS/4 ; LS/2 and LS).

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.238 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 0,223-0,253 mg/l

Effect concentrations 2

Duration:

48 h

Dose descriptor:

other: EC20

Effect conc.:

0.19 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 0,18-0,21 mg/l

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

0.17 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 0,16-0,18 mg/l

Results with reference substance (positive control):

August 6th, 2010 EC50 = 0,94 mg/L [0,89 – 0,99]
August 20th, 2010 EC50 = 0,94 mg/L [0,89 – 0,99]
Proficiency Test (2 times per year) A.G.L.A.E.

Any other information on results incl. tables

First definitive test

 Start of experimentation: August 3^rd, 2010 (D0)

End of experimentation: August 5^th, 2010 (D2)

 

Chromite de Cuivre Nominal Concentration (mg/L)	Mortality Daphnids at 24H *				Mortality (%)
	A	B	C	D
control	0	0	0	0	0
LS/80	0	0	0	0	0
LS/40	0	0	1	0	5
LS/20	0	0	1	0	5
LS/10	0	1	0	0	5
LS/5	0	0	0	1	5

   * 5 mobile daphnids per replicate at t=0

 

Chromite de CuivreNominal Concentration (mg/L)	Mortality Daphnids at 48H *				Mortality (%)
	A	B	C	D
control	0	0	0	0	0
LS/80	0	0	0	0	0
LS/40	1	0	1	0	10
LS/20	0	0	1	0	5
LS/10	0	1	1	0	10
LS/5	1	1	0	2	20

   * 5 mobile daphnids per replicate at t=0

 

Regarding these results and the normative requirements, the chosen theoretical concentration for the definitive test was LS/16, LS/8, LS/4, LS/2, LS.

Second definitive test

Start of experimentation: August 25^th, 2010 (D0)

End of experimentation: August 27^th, 2010 (D2)

 

 

Chromite de Cuivre initial Concentration (mg/L)	Mortality Daphnids at 24H *				Mortality (%)
	A	B	C	D
control	0	0	0	0	0
0,156	0	0	0	0	0
0,278	0	2	0	0	10
0,512	4	5	4	3	80
1,092	5	5	5	5	100
2,035	5	5	5	5	100

   * 5 mobile daphnids per replicate at t=0

 

Chromite de Cuivreinitial Concentration (mg/L)	Mortality Daphnids at 48H *				Mortality (%)
	A	B	C	D
control	0	1	0	0	0
0,156	0	1	0	0	5
0,278	3	4	3	4	70
0,512	5	5	5	5	100
1,092	5	5	5	5	100
2,035	5	5	5	5	100

   * 5 mobile daphnids per replicate at t=0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In a 48-hour semi-static acute toxicity test with Daphnia magna, the EC50 of Chromite de Cuivre was 0.238 mg/L.

Executive summary:

The objective of this study was to assess the acute toxicity of Chromite de cuivre diluted in an aqueous test medium, to a micro-crustacean species, Daphnia magna, in a 48‑hour semi‑static test (definitive test).

 

Results are described below.

 Results according to OECD 202 dated on April 13^th, 2004:

	Chromite de Cuivre Batch 09/0177
	BPL10-0010.001
EC50-48h	0,238 mg/l
Confidence limits (95%)	0,223-0,253 mg/l
EC20-48h	0,19 mg/l
Confidence limits (95%)	0,18-0,21 mg/l
EC10-48h	0,17 mg/l
Confidence limits (95%)	0,16-0,18 mg/l

 Legend : EC : Effective Concentration corresponding to the mobility inhibition concentration.