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EC number: 203-744-6 | CAS number: 110-18-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-10-30 to 2018-01-11
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- adopted 2011-07-28
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Identity: TMEDA, termed ATMEDAHP in the report
Purity: 99.9%
Lot number: 17F-1069978
C content: 62.01% (calculated)
Appearance: Liquid / colourless to yellow - Analytical monitoring:
- yes
- Details on sampling:
- Samples of test media were taken at test initiation (0 h) and termination (72 h) for the analysis of TMEDA concentrations, to confirm that nominal exposure concentrations were achieved and satisfactorily maintained during the test. Samples were stored deep frozen during the interval between collection and preparation for analysis.
- Vehicle:
- no
- Details on test solutions:
- A weighed quantity of TMEDA was dissolved in AAP algal growth medium to prepare a primary 1000 mg/L stock solution. The primary stock was serially diluted with AAP medium to prepare a range of test media with nominal concentrations of 0.954, 3.05, 9.77, 31.3 and 100 mg TMEDA/L. Unamended AAP medium served as the control treatment.
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The test was inoculated from a sterile preculture in exponential growth phase established at the test facility from a culture originally obtained from MBM Sciencebridge GmbH, Goettingen, Germany.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- Standard duration.
- Post exposure observation period:
- None, not a standard test requirement.
- Hardness:
- Not reported.
- Test temperature:
- 22.9 to 23.3 degrees C.
- pH:
- 0h: 7.62 (control), 8.40, 8.46, 8.92, 8.99 and 9.31 in TMEDA treatments in ascending concentration order.
72h: 7.95 (control), 7.90, 7.89, 7.97, 8.18 and 8.25 in TMEDA treatments in ascending concentration order - Dissolved oxygen:
- Not relevant.
- Salinity:
- Not relevant (freshwater test).
- Conductivity:
- Not reported.
- Nominal and measured concentrations:
- See Table 6.1.5-1 below.
- Details on test conditions:
- Test media were continuously shaken on an incubated oscillating platform to maintain algal cells in suspension beneath continual illumination (75.9 microEinsteins/square metre/sec) provided by fluorescent lighting.
- Reference substance (positive control):
- yes
- Remarks:
- The most recent reference test with potassium dichromate prior to this study with TMEDA was performed during July 2017.
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 20.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 11.7 to 37.9 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 4.82 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 1.09 to 9.03 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 9.41 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL: 4.46 to 16.2 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.09 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL: 0.123 to 2.71 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.954 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- Measured initial TMEDA concentrations ranged from 104% to 113% of nominal and concentrations in aged samples recovered at the end of the test ranged from 108% to 124% of nominals. The biological response was therefore evaluated with reference to nominal concentrations.
- Results with reference substance (positive control):
- In the most recent reference test performed prior to this study with TMEDA, the 72h ErC50 was 1.23 mg K2Cr2O7/L.
- Reported statistics and error estimates:
- See table of endpoints (effect concentrations) above.
- Validity criteria fulfilled:
- yes
- Remarks:
- See 'overall remarks', above.
- Conclusions:
- The effects of TMEDA on the growth of the unicellular freshwater alga Pseudokirchneriella subcapitata were determined in a 72-hour static test performed according to OECD 201 (2011) and in compliance with GLP. The 72-h ErC50 causing a 50% reduction in specific growth rate compared to the unamended control was 20.5 mg TMEDA/L (95% CL: 11.7 to 37.9 mg/L). The ErC10 was 4.82 mg/L and the corresponding NOErC was 0.954 mg/L.
- Executive summary:
Cells of the unicellular freshwater alga Pseudokirchneriella subcapitata were exposed for 72 h to TMEDA dissolved in AAP algal growth medium at nominal concentrations of 0 (control), 0.954, 3.05, 9.77, 31.3 and 100 mg/L. There were six replicate test vessels for the control group and three for each test substance treatment, each a 100 mL Erlenmeyer flask containing ca 50 mL medium initially inoculated with 5300 cells/mL. Test vessels were continually shaken to maintain cells in suspension and incubated under continuous illumination. Samples were taken from each flask after 24, 48 and 72 h to measure photosynthetic pigment fluorescence. Fluorescence measurements were converted to algal cell densities using a calibration established against direct cell counts made with a microscope and Neubauer chamber. Samples of test media were taken at test initiation and termination and analysed for TMEDA to confirm nominal exposure concentrations. Samples of media were taken from each treatment at 72 h and examined microscopically for evidence of morphological changes in the algal cells. A separate test was performed to determine the sensitivity of the stock culture to the reference toxicant potassium dichromate.
Analysis confirmed that exposure concentrations were achieved and maintained within 20% of nominals and biological effects were therefore evaluated on the basis of nominal exposure concentrations. Algal growth in the control replicates met the validity criteria of the guideline and the response to the reference toxicant was within the normal range; the observed response to TMEDA may therefore be considered reliable.
Statistically significant reductions in growth rate and cell yield occurred at concentrations >/= 3.05 mg TMEDA/L. No morphological abnormalities were observed in algal cells exposed to TMEDA and no detritus of ruptured cells was seen in any of the test media at 72 h. The 72 h ErC50 based on reduction of growth rate relative to the untreated control was 20.5 mg TMEDA/L (95% CL: 11.7 to 37.9 mg/L). The ErC10 was 4.82 mg/L and the corresponding NOErC was 0.954 mg/L.
Reference
Table 6.1.5 -1: Concentrations of TMEDA in a test of growth inhibition of P. subcapitata
Nominal TMEDA concentration (mg/L) |
Measured TMEDA concentration in algal growth medium |
|||
0 h (fresh) |
72 h (aged) |
|||
0 (control) |
< LOD |
- |
< LOD |
- |
0.954 |
1.08 |
[113] |
1.13 |
[118] |
3.05 |
3.40 |
[111] |
3.32 |
[109] |
9.77 |
11.0 |
[113] |
10.6 |
[108] |
31.3 |
35.2 |
[112] |
38.7 |
[124] |
100 |
104 |
[104] |
112 |
[112] |
LOD: 0.0270 mg TMEDA/L LOQ: 0.0900 mg TMEDA/L |
Table 6.1.5-2: Algal cell densities and inhibition of growth rate and cell yield of P. subcapitata following 72-h exposure to TMEDA under static conditions
Nominal TMEDA concn (mg/L) |
Mean algal cell density |
Inhibition of growth rate (%) |
Inhibition of cell yield (%) |
|||||||
0 h |
24 h |
48 h |
72 h |
0-24 h |
0-48 h |
0-72 h |
0-24 h |
0-48 h |
0-72 h |
|
0 |
0.53 |
1.26 |
5.40 |
24.64 |
- |
- |
- |
- |
- |
- |
0.954 |
0.53 |
1.23 |
4.78 |
22.88 |
1.4 |
4.7 |
1.6 |
4.1 |
12.7 |
7.3 |
3.05 |
0.53 |
1.09 |
3.70 |
17.96 |
18.7 |
16.3 |
8.9* |
23.3 |
34.9 |
27.7* |
9.77 |
0.53 |
0.93 |
3.12 |
14.31 |
40.0 |
25.5 |
14.8* |
45.2 |
46.8 |
42.8* |
31.3 |
0.53 |
0.45 |
0.84 |
2.42 |
136.3 |
88.6 |
71.4* |
111.0 |
93.6 |
92.2* |
100 |
0.53 |
0.20 |
0.37 |
0.89 |
237.3 |
122.7 |
91.3* |
145.2 |
103.3 |
98.5* |
* Statistically significant difference from the mean control |
Table 6.1.5‑3: Analysis of control replicate growth data and derivation of coefficients of variation (CV) for test validity assessment
Control |
Daily growth rate (d‑1) |
|||||||
mean |
|
mean, section-by-section |
||||||
0-24 h |
0-48 h |
0-72 h |
0-72 h St Dev |
0-72 h CV (%) |
0-24 h |
24-48 h |
48-72 h |
|
rep. 1 |
0.90240 |
1.22267 |
1.32158 |
0.36321 |
27 |
0.90240 |
1.54294 |
1.51941 |
rep. 2 |
0.89228 |
1.13580 |
1.30299 |
0.37837 |
29 |
0.89228 |
1.37933 |
1.63737 |
rep. 3 |
0.82774 |
1.20886 |
1.27498 |
0.39796 |
31 |
0.82774 |
1.58999 |
1.40722 |
rep. 4 |
1.09861 |
1.19692 |
1.32566 |
0.24369 |
18 |
1.09861 |
1.29523 |
1.58313 |
rep. 5 |
0.54430 |
0.96837 |
1.17083 |
0.55028 |
47 |
0.54430 |
1.39245 |
1.57575 |
rep. 6 |
0.84157 |
1.16704 |
1.24700 |
0.35371 |
28 |
0.84157 |
1.49252 |
1.40691 |
Mean |
0.85115 |
1.14994 |
1.27384 |
- |
30 |
0.85115 |
1.44874 |
1.52163 |
Std Dev |
0.17908 |
0.09426 |
0.05856 |
- |
- |
0.17908 |
0.11162 |
0.09629 |
CV (%) |
21.0 |
8.2 |
4.6 |
- |
- |
21.0 |
7.7 |
6.3 |
Description of key information
The effects of TMEDA on the growth of the unicellular freshwater alga Pseudokirchneriella subcapitata were determined in a 72-hour static test performed according to OECD 201 (2011) and in compliance with GLP. The 72-h ErC50 causing a 50% reduction in specific growth rate compared to the untreated control was 20.5 mg TMEDA/L (95% CL: 11.7 to 37.9 mg/L). The ErC10 was 4.82 mg/L and the corresponding NOErC was 0.954 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 20.5 mg/L
- EC10 or NOEC for freshwater algae:
- 4.82 mg/L
Additional information
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