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EC number: 242-833-4 | CAS number: 19139-31-2
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- starting date: 07.07.1999, completion date: 05.08.1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- not specified
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Commercial name: DIHEXYL FUMARATE
Chemical nature: dihexyl butenedioate (trans)
Empirical formula: C16H28O4
Molecular weight: 284.4 g/mol
CAS Number: 19139-31-2
Physical-chemical and analytical data
-General
Aspect: colourless liquid
Density: 0.953 g/ml at 20 °C
-Sample tested
Lot No.: 9000343320
Purity (GLC): 98.5 % - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (City of Geneva, Aire) was used.
The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day. - Duration of test (contact time):
- 29 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (O2 consumption)
- Value:
- 89
- Sampling time:
- 29 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 88
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- DIHEXYL FUMARATE undergoes 89 % biodegradation after 29 days (88 % after 28 days) in the test conditions. Biodegradation starts on day 1 and reaches 68 % at the end of the 10-day window (days 1 to 11).
Thus, DIHEXYL FUMARATE should be regarded as readily biodegradable according to this test.
Reference
Oxygen uptakes, as read on the SAPROMAT meters, are corrected:
- by deducting the basic oxygen uptake of sludge (flasks 1/3 and 1/4)
- proportionally to account for the small differences between actual and nominal concentrations of test and reference substances.
Degradation of sodium benzoate exceeds 40 % after 7 days and 65 % after 14 days: the activity of the inoculum is thus verified and the test is considered as valid.
BIODEGRADABILITY
Biological Oxygen Demand (BOD, mg O2/L, adjusted to nominal concentrations):
Days: |
1 |
|
7 |
11 |
21 |
28 |
29 |
||
BOD |
1st flask |
B1 |
0.0 |
|
10.0 |
12.0 |
14.0 |
16.0 |
16.0 |
Sludge |
2nd flask |
B2 |
0.0 |
|
10.0 |
14.0 |
14.0 |
16.0 |
16.0 |
|
mean |
B |
0.0 |
|
10.0 |
13.0 |
14.0 |
16.0 |
16.0 |
|
1st flask |
C1 |
23.8 |
|
139.1 |
175.9 |
215.6 |
225.6 |
227.6 |
BOD |
2nd flask |
C2 |
24.4 |
|
133.2 |
169.4 |
211.5 |
221.3 |
223.3 |
Test |
1st fl. corr. |
C1-B |
23.8 |
|
129.1 |
162.9 |
201.6 |
209.6 |
211.6 |
Subst. |
2nd fl. corr. |
C2-B |
24.4 |
|
123.2 |
156.4 |
197.5 |
205.3 |
207.3 |
% |
1rst flask |
D1 |
10 |
|
55 |
69 |
85 |
89 |
90 |
degr. |
2nd flask |
D2 |
10 |
|
52 |
66 |
84 |
87 |
88 |
|
mean |
D |
10 |
|
53 |
68 |
85 |
88 |
89 |
B = (B1 + B2) / 2
D1 = 100 * (C1 - B) / ThOD * [S]
D2 = 100 * (C2 - B) / ThOD * [S]
D = (D1 + D2) / 2
[S] : Initial test substance concentration (mg/I)
DIHEXYL FUMARATE undergoes 89 % biodegradation after 29 days (88 % after 28 days) in the test conditions. Biodegradation starts on day 1 and reaches 68 % at the end of the 10-day window (days 1 to 11).
Thus, DIHEXYL FUMARATE should be regarded as readily biodegradable according to this test.
The curves obtained with the reference substance alone and with DIHEXYL FUMARATE + reference substance show no toxic effect of DIHEXYL FUMARATE to the micro-organisms at the test concentration.
Description of key information
DIHEXYL FUMARATE undergoes 89 % biodegradation after 29 days (88 % after 28 days) in the test conditions. Biodegradation starts on day 1 and reaches 68 % at the end of the 10-day window (days 1 to 11).
Thus, DIHEXYL FUMARATE should be regarded as readily biodegradable according to this test.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
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