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EC number: 257-048-2 | CAS number: 51200-87-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 November - 14 December 2001
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 1984
- Deviations:
- yes
- Remarks:
- see "Principles of method if other than guideline"
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)
- Version / remarks:
- 1996
- Deviations:
- yes
- Remarks:
- see "Principles of method if other than guideline"
- Principles of method if other than guideline:
- Between the 72- and 96-hour cell counts in the range-finding test, the temperature in the growth cabinet increased to 27°C for a maximum of 10 hours, which is above the recommended temperature range of 24+-1°C.
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4,4-dimethyloxazolidine
- EC Number:
- 257-048-2
- EC Name:
- 4,4-dimethyloxazolidine
- Cas Number:
- 51200-87-4
- Molecular formula:
- C5H11NO
- IUPAC Name:
- 4,4-dimethyl-1,3-oxazolidine
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- - Name: BIOBAN CS-1135 (4,4-dimethyl-oxazolidine)
- Source and lot/batch No.of test material: Dow Chemical Company, 1H25 DF87
- Purity: 76%
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- Samples of each test concentration and the control were collected in amber glass jars at time 0,72 and 96 hours. Samples, along with appropriate chain-of custodies, were transported to Maxxam Analytics Inc. for chemical analyses.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION OF TEST SOLUTIONS
Range-finding test: A stock solution of 1000 mg/L was prepared by mixing 101.3 mg of the test substance in 100 mL of filtered nutrient solution. The solution was then placed on a magnetic stir plate and agitated for just over 4 hours.
Definitive test: A 10 mg/L stock solution was prepared, by mixing 10.1 mg of the test substance in 1000 mL filtered nutrient solution and stirring for 10 minutes.
Test organisms
- Test organisms (species):
- Selenastrum sp.
- Details on test organisms:
- TEST ORGANISM
- Test species: Uni-algal cultures of Selenastrum capricornutum Printz 1913
- Source (laboratory, culture collection): Axenic slope culture of Selenastrum capricornutum, cultured at the ESG International Ecotoxicity Laboratory (Guelph, ON) since 1999.
- Method of cultivation: Cultures are aseptically transferred twice weekly and maintained in temperature and light controlled environments isolated from all testing facilities. Algal growth curves are conducted semi-annually to ensure that algae are in an exponential growth phase.
- Age of test plants: 3-7 days
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- not reported
- Test temperature:
- 24 +- 1°C
- pH:
- 7.5+-0.1
- Dissolved oxygen:
- not reported
- Salinity:
- not reported
- Conductivity:
- not reported
- Nominal and measured concentrations:
- Range-finding test:
Exposure concentrations one order of magnitude apart, were used to determine the range of concentrations required for definitive testing with S. capricornutum. the highest concentration tested was 1,000 mg/L, together with four lower concentrations (i.e. 100 mg/l, 10 mg/L, 1 mg/L, 0.1 mg/L) and a control (100% nutrient solution). S. capricornutum cells (1*10^4 cells/mL +- 1000 cells/mL) were added to each flask (1 replicate per concentration). As per test method, the exposure duration was 96 hours, with daily cell counts.
Definitive testing
A definitive multi-concentration EC50 test was conducted with nominal concentrations of 0.625, 1.25, 2.5, 5 and 10 mg/L plus controls. Each concentration was replicated 5 times. Observations of cell number and appearance were conducted, using a haemocytometer, on a daily basis. After 72 hours, the pH in one replicate sample was measured and the sample was sent for analysis, leaving four replicated remaining for the 96-hour counts. Changes in cell development or appearance, such as cell clumping, cell morphology, cell colour, cell shape, cell size etc. were reported. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass flasks with 50 mL of test medium, covered with Jaece non-toxic foam plugs
- Culture medium: Algal growth medium; axenically subcultured into fresh medium twice a week
- Testing medium: Algal growth medium; filter sterilized, but otherwise treated in a non-axenic manner
- Light quality: cool-white fluorescent
- Light intensity: 4+-10% kLux for culturing; 8+-20% kLux for testing
- Photoperiod: 24 hr
- Number of cells per test vessel: 1*10^4 cells/mL
PARAMETERS MEASURED
- Measured endpoints: cell number measured daily using a haemocytometer
- Calculated endpoints: Area under the growth curve, growth rate, cell number
- Statistical endpoints: 72 and 96-hr ECb50 (based on area under the growth curve), 72 and 96-hr ECr50 (based on growth rate), 72 and 96-hr EC50 for cell number, LOEC and NOEC for all three biological endpoints
- number of test concentrations: 5 concentrations, plus control - Reference substance (positive control):
- yes
- Remarks:
- Sodium chloride as reference toxicant
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.79 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 2.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.57 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- Range-finding test:
Results indicated that the 50% inhibitory effect level and the no effect level for growth inhibition of S. capricornutum was between 1 and 10 mg/L for all endpoint parameters (72 and 96-hour cell number, area under the growth curve and growth rate).
Definitive test:
Results are showed in the table in section "any other information on results".
Any other information on results incl. tables
Test endpoint | EC50 (95% Confidence Intervals) (mg/L) | NOEC (mg/L) | LOEC (mg/L) |
Cell number (72 hr) | 3.79 (3.51, 4.07) | 1.25 | 2.50 |
Cell number (96 hr) | 5.42 (4.84, 5.98) | 2.50 | 5.00 |
Area under growth curve (72 hr ECb50) | 3.76 (3.46, 4.02) | 1.25 | 2.50 |
Area under growth curve (96 hr ECb50) | 4.20 (3.73, 4.69) | 2.50 | 5.00 |
Growth rate (0 -72 hr ECr50) | 6.57 (6.26, 6.92) | 2.50 | 5.00 |
Growth rate (0 -96 hr ECr50) | 7.14 (6.95, 7.31) | 2.50 | 5.00 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The ecotoxicological evaluation of the effects of 4,4-dimethyl-oxazolidine (CAS 51200-87-4) indicated that the 72 and 96 -hour EC50s for cell number were 3.79 and 5.42 mg/L, respectively. The 72 and 96 -hour EC50s for area under the growth curve were 3.76 and 4.20 mg/L, respectively. The 0 to 72 and 0 to 96 -hour EC50s for growth rate were 6.57 and 7.14 mg/L, respectively. The NOEC and LOEC for 72 -hour cell number and area under the growth curve were 1.25 and 2.50 mg/L, respectively. For the other four endpoint parameters, the NOEC and LOEC were 2.50 and 5.00 mg/L, respectively.
- Executive summary:
Ecotoxicological testing of Bioban CS-1135 (CAS 51200 -87 -4) was conducted to determine the growth inhibition of the freshwater green alga, Selenastrum capricornutum Printz, according to the OECD Method 201 (Alga, Growth Inhibition Test, 1984) and in general accordance with OPPTS Method 850.5400 (Algal Toxicity, Tiers I and II, 1996).
Results from the range-finding test indicated that the 50% inhibitory effect level and the no effect level for growth inhibition of Selenastrum capricornutum were between 1 and 10 mg/L for all endpoint parameters (72 and 96 -hour cell number, area under growth curve and growth rate).
The available analytical methods for the test item were not able to extract and quantify this compound. Calculations of all statistical endpoints for the definitive test were conducted using nominal concentrations. The 72 and 96 -hour EC50s for cell number were 3.79 and 5.42 mg/L, respectively. The 72 and 96 -hour EC50s for area under the growth curve were 3.76 and 4.20 mg/L, respectively. The 0 to 72 and 0 to 96 -hour EC50s for growth rate were 6.57 and 7.14 mg/L, respectively. The NOEC and LOEC for 72 -hour cell number and area under the growth curve were 1.25 and 2.50 mg/L, respectively. For the other four endpoint parameters, the NOEC and LOEC were 2.50 and 5.00 mg/L, respectively.
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