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EC number: 947-988-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 April 2004
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of 2,2'-thiodiethanethiol and 1095071-01-4 and α-sulfanyl-ω-{2-[(2-sulfanylethyl)sulfanyl]ethyl}bis[ethane- 1,2-diylsulfanediylethane- 1,2-diylsulfanediyl(3-hydroxypropane-1,1-diyl) sulfanediyl]
- EC Number:
- 947-988-8
- Molecular formula:
- not applicable
- IUPAC Name:
- Reaction mass of 2,2'-thiodiethanethiol and 1095071-01-4 and α-sulfanyl-ω-{2-[(2-sulfanylethyl)sulfanyl]ethyl}bis[ethane- 1,2-diylsulfanediylethane- 1,2-diylsulfanediyl(3-hydroxypropane-1,1-diyl) sulfanediyl]
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- HPLC
- Details on sampling:
- - Concentrations: nominal test concentrations of 8.0, 3.64, 1.66, 0.74 and 0.34 mg/L (factor 2.2) and a control were tested
- Sampling method: Duplicate samples from the freshly prepared test media of all test concentrations and the control were taken at the start of the test.
For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentrations during the test period, duplicate samples from the test media of all test concentrations and the control were collected at the end of the test (after 48 hours) by pouring together the contents of the test beakers of each treatment.
All samples were diluted by a factor of two with acetonitrile.
Additional samples of the control and the dilution solvent acetonitrile were taken at each sampling without any sample treatment.
- Sample storage conditions before analysis: All samples were stored in a freezer (≤ - 20 °C), protected from light, until analysis was performed. Afterwards the samples were again stored deep frozen (≤ -20 °C) and will be kept stored up to the date of the final report.
Test solutions
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item is not well soluble in test medium. To avoid physical effects of undissolved test item on the daphnids, no concentrations above the solubility limit of the test item in test water was tested.
Therefore, a supersaturated stock solution of nominal 8 mg test item/L was prepared by suspending 12.3 mg test item in 1537.5 mL test water. The stock suspension was stirred for 24 hours at room temperature in the dark to dissolve as much test item as possible.
Then, non-dissolved fractions of the test item were separated from the test medium by membrane filtrations (0.65 µm and 0.45 µm cellulose acetate filter). The solution with dissolved test item was used as the test medium of the highest test concentration and to prepare the desired 1:2.2, 1:4.8, 1:10.8 and 1:23.5 dilutions.
The test media were prepared just before introduction of the daphnids (= start of the test).
- Test concentration separation factor: 2.2
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): There were no remarkable observations, except for the stock solution, where particles were swimming at the surface before filtration.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Daphnia magna (Straus), clone 5
- Age at study initiation (mean and range, SD): From 0.5 to 22.0 hours old
- Method of breeding: The daphnids were bred in the laboratories of ibacon under similar temperature and light conditions as used in the test. The cultivation of the parental daphnids was performed in Elendt M4 medium. The test organisms were not first brood progeny. The daphnids in the stock culture were fed at least on all working days with green algae (Desmodesmus subspicatus) freshly grown in the laboratories of ibacon.
- Feeding during test: none
ACCLIMATION
- Acclimation period: Was not necessary, since the test was performed in the same medium as the culturing
- Acclimation conditions (same as test or not): as test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.5 mmol/L (= 250 mg/L) as CaCO 3
- Test temperature:
- 20.6 to 20.7 °C at test start;
20.1 to 20.6 °C at test end - pH:
- 7.9 to 8.2 at test start;
7.9 at test end; and thus the pH-value did not vary by more than 1.5 units - Dissolved oxygen:
- 8.9 to 9.1 mg/L at test start;
8.6 to 8.8 mg/L at test end - Conductivity:
- < 5 µScm -1
- Nominal and measured concentrations:
- Nominal: 8.00, 3.64, 1.66, 0.74, and 0.34 mg/L
arithmetic mean measured: 4.00, 2.02, 0.896, 0.443 and 0.125 mg/L. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers
- The test vessels were covered with a lid to reduce the loss of water due to evaporation and to avoid the entry of dust into the solutions.
- Material, size, headspace, fill volume: Glass, 100 mL, fill volume: 60 mL
- Aeration: No
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted Water (Elendt "M4")
- Alkalinity: 0.9 mmol/L
- Conductivity: < 5 µScm -1
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8
- Light intensity: 410 to 830 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The mobility of the daphnids was determined by visual observation after 24 and 48 hours. Those animals that are not able to swim within 15 seconds after gentle agitation of the test beaker were considered to be immobile (even if they could still move their antennae).
VEHICLE CONTROL PERFORMED: no
RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study:
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 4 mg/L
- 95% CI:
- >= 3.12 - <= 4
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Any other information on results incl. tables
Mean measured concentration [mg test item/L] | No. of Daphnia tested | No. of immobilized Daphnia after | % of immobilised Daphnia after | ||
24 h | 48 h | 24 h | 48 h | ||
Control | 20 | 0 | 0 | 0 | 0 |
0.125 | 20 | 0 | 0 | 0 | 0 |
0.443 | 20 | 0 | 0 | 0 | 0 |
0.896 | 20 | 0 | 0 | 0 | 0 |
2.02 | 20 | 0 | 2 | 0 | 10 |
4.00 | 20 | 0 | 6 | 0 | 30 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxic effect of the test item HIDT to Daphnia magna was assessed in a static concentration-response test. The 48-hour NOEC was determined to be 0.896 mg test item/L. The 48-hour LOEC was determined to be 2.02 mg test item/L and the 48-hour EC50 value was determined to be > 4.00 mg test item/L. The initial concentrations and the maintenance of the exposure concentrations during the test were verified in the analytical part. All reported results refer to mean measured values since a filtrate was used and the concentrations of the test item were not within ± 20% of the nominal concentrations during the test.
- Executive summary:
The 48 –hr-acute toxicity of 1-propanol, 3,3-bis[[2-[(2-mercaptoethyl)thio]ethyl]thio]- to Daphnia magna was studied under static conditions. Test species were exposed to control and test chemical at (nominal/measured) concentration of 0.34, 0.74, 1.66, 3.64, and 8.00 mg a.i./L and 0.125, 0.443, 0.896, 2.02, and 4.00 mg a.i./L for 48 hr. Immobilisation and sublethal effects were observed daily. The 48 – h EC50 was > 4.00 mg a.i./L (> 8.00 mg a.i/L nominal). The 48 – h NOEC based on immobilization was 0.896 mg a.i./L (1.66 mg/L nominal).
Based on the results of this study, {test material} would be not classified as acutely toxic to Daphnia magna in accordance with Regulation (EU) No. 1272/2008 (CLP).
Results Synopsis
Test Type (Static):
EC50: > 4.00 mg a.i./L 95% C.I. : {3.12 to 4.00 mg a.i./L}
EC50: > 8.00 mg a.i./L nominal
NOEC: 0.896 mg a.i./L}
NOEC: 1.66 mg a.i./L} nominal
Endpoint(s) Effected: Mobility
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