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EC number: 221-297-5 | CAS number: 3058-38-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- purity of test substance, no. of cells per culture, no. of replications, vehicle, signs of toxicity, evaluation and interpretation of results, individual plate counts not reported
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 976
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- purity of test substance, no. of cells per culture, no. of replications, vehicle, signs of toxicity, evaluation and interpretation of results, individual plate counts not reported
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- no
- Remarks:
- pre-GLP
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,4,6-trinitrobenzene-1,3,5-triamine
- EC Number:
- 221-297-5
- EC Name:
- 2,4,6-trinitrobenzene-1,3,5-triamine
- Cas Number:
- 3058-38-6
- Molecular formula:
- C6H6N6O6
- IUPAC Name:
- trinitrobenzene-1,3,5-triamine
- Test material form:
- not specified
- Details on test material:
- - Name of test material as cited in the report: TATB ; 1,3,5-triamino-2,4,6-trinitrobenzene
- Lot/batch No.of test material: B-318
Constituent 1
Method
- Target gene:
- Histidine and tryptophan for S. typhimurium and E. Coli, respectively.
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium, other: TA 1535, TA 1537, TA 1538, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- not applicable
- Additional strain / cell type characteristics:
- not applicable
- Species / strain / cell type:
- E. coli WP2
- Details on mammalian cell type (if applicable):
- not applicable
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-stimulated, rat liver-homogenate metabolic activation system was used
- Test concentrations with justification for top dose:
- 5, 10, 50, 100, 500, 1000, 2500 and 5000 μg/plate, with and without S9-mix in S. typhimurium TA 1535, TA 1537, TA 1538, TA 98 & TA 100 and E. coli WP2
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- other: 6-propiolactone (50 μg/plate for TA 1535); 2-Anthramine (20 μg/plate for TA 98, TA 100 and WP2)
- Remarks:
- without metabolic activation
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-Anthramine
- Remarks:
- with metabolic activation
- Details on test system and experimental conditions:
- SOURCE OF TEST SYSTEM: S. typhimurium strains were obtained from Dr. Bruce Ames of the University of California at Berkeley. E. coli WP2 was obtained from Dr. D. McCalla.
METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: Plates were incubated at 37 °C for 2 days. - Evaluation criteria:
- None
- Statistics:
- None
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium, other: TA 1535, TA 1537, TA 1538, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
Any other information on results incl. tables
Table 7.6.1/1: Ames test - results
Test substance |
Dose levels (µg/plate) |
Mean no. Of revertants per plate |
|||||
Histidine revertants |
Tryptophan revertants |
||||||
TA 1535 |
TA 1537 |
TA 1538 |
TA 98 |
TA 100 |
E. coli WP2 |
||
Without metabolic activation |
|||||||
Negative control |
- |
23 |
10 |
14 |
29 |
160 |
76 |
TATB |
5 |
41 |
13 |
15 |
28 |
151 |
- |
10 |
30 |
7 |
12 |
32 |
126 |
81 |
|
50 |
32 |
7 |
13 |
25 |
148 |
93 |
|
100 |
35 |
8 |
16 |
21 |
148 |
78 |
|
500 |
40 |
8 |
9 |
25 |
146 |
95 |
|
1000 |
25 |
10 |
14 |
25 |
125 |
69 |
|
2500 |
31 |
8 |
9 |
28 |
152 |
84 |
|
5000 |
33 |
8 |
14 |
30 |
125 |
87 |
|
Positive control |
* |
680 |
>2000 |
310 |
40 |
160 |
72 |
With metabolic activation |
|||||||
Negative control |
- |
17 |
11 |
24 |
57 |
156 |
81 |
TATB |
5 |
23 |
7 |
19 |
34 |
128 |
- |
10 |
20 |
9 |
28 |
25 |
151 |
91 |
|
50 |
24 |
10 |
22 |
42 |
138 |
108 |
|
100 |
22 |
7 |
20 |
39 |
132 |
87 |
|
500 |
20 |
13 |
21 |
34 |
145 |
88 |
|
1000 |
18 |
6 |
17 |
35 |
139 |
88 |
|
2500 |
20 |
8 |
16 |
27 |
143 |
88 |
|
5000 |
20 |
6 |
15 |
33 |
173 |
94 |
|
Positive control |
** |
- |
- |
- |
>2000 |
>2000 |
1170 |
*Without metabolic activation - 9-Aminoacridine (100 μg/plate for TA 1537); 2-Nitrofluorene (50 μg/plate for TA 1538); 6-propiolactone (50 μg/plate for TA 1535); 2-Anthramine (20 μg/plate for TA 98, TA 100 and WP2)
**With metabolic activation - 2-Anthramine (20 μg/plate for TA 98, TA 100 and E. coli WP2)
Applicant's summary and conclusion
- Conclusions:
- Under the test conditions, test substance is not considered as mutagenic in S. typhimurium (TA1535, TA1537, TA 1538, TA98 and TA100) and E. coli WP2 strains.
- Executive summary:
In a reverse gene mutation assay in bacteria, performed similarly to the OECD Guideline 471, strains of Salmonella typhimurium (TA1535, TA1537, TA 1538, TA98 and TA100) and Escherichia coli WP2 were exposed to test substance at the following concentrations using the plate incorporation method:
5, 10, 50, 100, 500, 1000, 2500 and 5000 μg/plate, with and without S9-mix
Negative and positive control groups were also included in mutagenicity tests.
The mean numbers of revertant colonies are fell within acceptable ranges for negative control treatments, and were elevated by positive control treatments.
No significant increases in the frequency of revertant colonies were recorded for any of the bacterial strains, at any dose level either with or without metabolic activation.
Under the test conditions, test substance is not considered as mutagenic in S. typhimurium (TA1535, TA1537, TA 1538, TA98 and TA100) and E. coli WP2 strains.
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