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Diss Factsheets
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EC number: 220-278-9 | CAS number: 2698-41-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vitro
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 442D (In Vitro Skin Sensitisation: ARE-Nrf2 Luciferase Test Method)
- Version / remarks:
- This study is carried out according the OECD Guideline 442D dated February, 04th, 2015, the ECVAM DBALM protocol 155: KeratinoSensTM, C(81)30 OECD Guidelines and the article annex II to article D-523-8 of the French Environment
- Deviations:
- not specified
- GLP compliance:
- yes
- Remarks:
- not mentioned on study reports but on IDEAtest group website www.groupeideatests.com
- Type of study:
- activation of keratinocytes
- Justification for non-LLNA method:
- Alternative study to in-vivo as the first intention, in accordance with REACH annex VII and the Adverse Outcome Pathway (AOP) for Skin Sensitisation Initiated by Covalent Binding to Proteins (OECD, 2014)
Test material
- Reference substance name:
- [(2-chlorophenyl)methylene]malononitrile
- EC Number:
- 220-278-9
- EC Name:
- [(2-chlorophenyl)methylene]malononitrile
- Cas Number:
- 2698-41-1
- Molecular formula:
- C10H5ClN2
- IUPAC Name:
- [(2-chlorophenyl)methylene]malononitrile
- Reference substance name:
- unknown impurities
- Cas Number:
- Not applicable
- Molecular formula:
- Not applicable
- IUPAC Name:
- unknown impurities
- Test material form:
- solid
Constituent 1
impurity 1
In vitro test system
- Details on the study design:
- see OECD 442D guidelinae and enclosed study plan
Results and discussion
- Positive control results:
- Cinnamaldehyde EC1.5 7.92 and Imax 7.64
In vitro / in chemico
Resultsopen allclose all
- Key result
- Run / experiment:
- other: mean on 2 runs
- Parameter:
- other: IC70 is concentration in μM for which we obtained 70% cell viability
- Value:
- 23.31
- Vehicle controls validity:
- not examined
- Remarks:
- control solvent as negative control
- Negative controls validity:
- valid
- Remarks:
- control solvent
- Positive controls validity:
- valid
- Remarks:
- Cinnamaldehyde Imax is 7.64
- Remarks on result:
- positive indication of skin sensitisation
- Key result
- Run / experiment:
- other: Linear EC1.5 μM and EC1.5 Lin/Log μM
- Parameter:
- other: EC1.5, value representing the concentration for which induction of luciferase activity is above 1.5 threshold was obtained
- Value:
- 2.97
- Vehicle controls validity:
- not examined
- Remarks:
- constrol solvent as negative control
- Negative controls validity:
- valid
- Remarks:
- control solvent
- Positive controls validity:
- valid
- Remarks:
- cinnamaldehyde EC1.5 is 7.92 μM
- Remarks on result:
- positive indication of skin sensitisation
- Other effects / acceptance of results:
- Since all validity criteria are met, study is considered as valid.
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (skin sensitising) based on GHS criteria
- Conclusions:
- In both repetition, Imax is higher than 1.5, the EC1.5 is lower than 1000 μM and, at the EC1.5 concentration, viability is higher than 70%.
Under the retained experimental conditions, the test item may be classified as sensitizer. - Executive summary:
In vitro sensitization KeratinoSens™ assay OECD 442D is performed with Keratinocytes based on the signaling pathway Keap1-Nrf2-ARE coupled to the luciferase reporter gene. Under the retained experimental conditions, the test item may be classified as sensitizer.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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