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EC number: 948-034-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
Please refer to the Read-across statement attached under section 13.
1. HYPOTHESIS FOR THE ANALOGUE APPROACH
This Read-Across is based on the hypothesis that the target and the source substances have similar environmental fate and (eco)toxicological properties because both substances have the same common compound octyl sulfonate while another main constituent of the target substance octyl disulfonate is considered to have similar level of toxicity as octyl sulfonate. Other non-common compounds represented by impurities are considered not to influence the read-across validity because they are either structurally identical in the target and in the source substances or, if different, do not contribute to the toxicity effects because they are also anionic sulfonates with the same functional groups and their content is very low.
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
Please refer to the Read-across statement attached under section 13.
3. ANALOGUE APPROACH JUSTIFICATION
The aquatic toxicity of the ANS category members is influenced mainly by the length of alkyl chain. Numerous studies demonstrate increasing toxicity with the chain length. Since all sulfonate containing constituents of the target substance are octyl derivatives, they are considered not to possess higher toxicity potential to aquatic environment than the source substance. Taking into account the similar predicted environmental fate and behavior as well as the similar mode of ecotoxic action due to its surfactant properties, the second sulfonate group of the main constituent octyl disulfonate is considered not to result in a higher toxicity to aquatic life than the source substance. Thus, the results of the acute toxicity study in algae available for the source substance are considered to be the same if a study with the target substance was conducted.
4. DATA MATRIX
Please refer to the Read-across statement attached under section 13. - Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: predicted result from the source substance
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: predicted result from the source substance
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: predicted result from the source substance
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Remarks on result:
- other: predicted result from the source substance
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Remarks on result:
- other: predicted result from the source substance
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Remarks on result:
- other: predicted result from the source substance
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: predicted result from the source substance
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Remarks on result:
- other: predicted result from the source substance
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Basis for effect:
- growth rate
- Remarks on result:
- not determinable
- Remarks:
- No statistically significant inhibitory effects
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Basis for effect:
- other: yield
- Remarks on result:
- not determinable
- Remarks:
- No statistically significant inhibitory effects
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): Microscopically observed at the end of the test, all cells were considered normal for the control and all test item concentrations.
- Unusual cell shape: None
- Colour differences: None
- Flocculation: None
- Adherence to test vessels: None reported
- Aggregation of algal cells: None reported
- Other: N/A
- Any stimulation of growth found in any treatment:
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Effect concentrations exceeding solubility of substance in test medium: None - Results with reference substance (positive control):
- - Results with reference substance valid?
- EC50: Growth rate: 1.23 mg/L (nominal); Yield: 0.685 mg/L
- Other: The EC50 values calculated in the reference test (performed in a separate study twice a year by the laboratory) were considered to be within an acceptable range therefore it can be considered that the test organism is sensitive. - Reported statistics and error estimates:
- After 72 h at termination of the test no statistically significant inhibition of growth rate or yield was observed for test item concentrations up to and including 100 mg/L. Due to an inhibition below 10, 20 and 50 % for growth rate and yield, the calculation of the EC10, 20, 50 was not indicated.
The statistical evaluation for the 72 hours period was performed for growth rate and yield using SAS® (2002–2010). A test for normality of the data was performed by calculating the Shapiro-Wilk statistic and the homogeneity of variance of the data was evaluated by calculating the Levene Test. The NOEC and LOEC were determined by using a multiple comparison method (Dunnetts-t-test, two-tailed, for growth rate, Jonckheere Terpstra test, two-tailed, for yield). - Validity criteria fulfilled:
- yes
- Conclusions:
- The same result is predicted for the target substance.
No statistically significant inhibitory effects on any parameter (growth rate, yield) were observed at any of the source substance concentrations, including the highest test item concentration of 100 mg/L (nominal) at test end. Thus the overall LOEC was not determinable and the overall NOEC was observed to be at 100 mg/L (nominal).
The EC10, EC20 and EC50 for growth rate and yield were considered to be > 100 mg/L (nominal). - Executive summary:
This GLP-compliant study with the source substance sodium octane-1 -sulfonate was conducted in accordance with the OECD guideline 201; “Freshwater Alga and Cyanobacteria, Growth Inhibition Test" (28 July 2011), in order to determine the effects of the test item on the growth and yield of green alga Pseudokirchneriella subcapitata. Initial target cell densities of 0.5 × 104 cells/mL were employed for the individual replicates. The increase of cell numbers was assessed over a test period of 72 hours. A static test with nominal test item concentrations of 100, 31.3, 9.77, 3.05, 0.954 mg/L and control was performed. Six replicates were employed for the control and three for each test item concentration. The test was performed in 100 mL Erlenmeyer flasks each containing ~ 50 mL test solution. Analytical samples were analysed from control and test item concentration 100 mg/L at 0 hours (initial value) from fresh test solutions and after 72 hours from aged solutions. The measured initial concentration of the test item was 91.2 % of nominal in the highest test item concentration of 100 mg/L. In the aged samples the measured concentrations was 102.6 %.
No statistically significant inhibitory effects on any parameter (growth rate, yield) were observed at any test item concentrations up to the highest concentration tested (100 mg/L, nominal) at test end. No abnormalities in the cells were observed at test end.
The overall LOEC was not determinable and the overall NOEC was observed to be 100 mg/L (nominal). The EC10, EC20 and EC50 for growth rate and yield were considered to be > 100 mg/L (nominal).
Reference
Validity criteria of the study:
Biomass: Cell numbers, measured in the controls between 0 h and 72 hours, were found to increase by a factor of 54.20, which exceeds the threshold of 16. It corresponds to a growth rate of 1.32937 d-1.
Coefficient of Variation (section by section): The mean coefficient of variation for the section-by-section specific growth rates (hours 0 - 24, 24 - 48 and 48 - 72) in the control cultures was 20 % and did not exceed 35 %.
Coefficient of Variation (average growth rate): The coefficient of variation of average growth rate in replicate control cultures was 2.6 % and did not exceed 7 % for the whole test period.
Results:
After 72 h at termination of the test no statistically significant inhibition of growth rate or yield was observed for test item concentrations up to and including 100 mg/L
Table 2. Average cell numbers for each sampling time and concentration
Conc. |
Average cell numbers [104/mL] |
|||
[mg/L] |
0 h |
24 h |
48 h |
72 h |
Control |
0.55 |
1.61 |
7.18 |
29.81 |
0.954 |
0.55 |
2.03 |
8.01 |
32.76 |
3.05 |
0.55 |
2.14 |
9.79 |
38.78 |
9.77 |
0.55 |
2.31 |
13.54 |
58.10 |
31.3 |
0.55 |
2.33 |
14.55 |
56.73 |
100 |
0.55 |
1.95 |
14.51 |
62.16 |
Table 3. Percentage inhibition of growth rate
Conc. |
% Inhibition of growth rate1) |
||
[mg/L] |
0 h – 24 h |
0 h – 48 h |
0 h – 72 h |
9.5 |
-1.6 |
-4.4 |
|
-2.2 |
-7.3 |
0.3 |
|
Control |
10.9 |
4.2 |
3.4 |
11.4 |
8.5 |
1.2 |
|
-18.7 |
1.6 |
-0.9 |
|
-10.9 |
-5.4 |
0.4 |
|
Mean |
0.0 |
0.0 |
0.0 |
-18.7 |
-0.5 |
1.8 |
|
0.954 |
-21.5 |
-12.0 |
-7.0 |
-23.7 |
0.0 |
-1.4 |
|
Mean |
-21.3 |
-4.2 |
-2.2 |
-29.4 |
-13.7 |
-9.7 |
|
3.05 |
-27.7 |
-14.9 |
-4.3 |
-21.9 |
-8.2 |
-5.8 |
|
Mean |
-26.3 |
-12.3 |
-6.6 |
-31.4 |
-27.8 |
-19.0 |
|
9.77 |
-31.4 |
-26.1 |
-15.3 |
-37.0 |
-21.0 |
-16.1 |
|
Mean |
-33.3 |
-25.0 |
-16.8 |
-30.6 |
-28.6 |
-14.2 |
|
31.3 |
-38.6 |
-27.0 |
-17.0 |
-33.9 |
-27.9 |
-17.4 |
|
Mean |
-34.4 |
-27.8 |
-16.2 |
-18.7 |
-27.7 |
-17.8 |
|
100 |
-17.7 |
-28.4 |
-20.4 |
-16.3 |
-27.1 |
-17.2 |
|
Mean |
-17.6 |
-27.7 |
-18.5 |
Table 4. Percentage inhibition of yield
Conc. |
% Inhibition of yield1) |
||
[mg/L] |
0 h – 24 h |
0 h – 48 h |
0 h – 72 h |
3.8 |
-16.6 |
-34.1 |
|
-3.8 |
-21.2 |
1.8 |
|
Control |
15.1 |
10.4 |
11.8 |
18.9 |
23.3 |
6.9 |
|
-17 |
17.3 |
9.8 |
|
-17 |
-12.8 |
3.8 |
|
Mean |
0.0 |
0.1 |
0.0 |
-34 |
-0.3 |
7.4 |
|
0.954 |
-39.6 |
-37.7 |
-32.3 |
-44.3 |
1.1 |
-5.3 |
|
Mean |
-39.3 |
-12.3 |
-10.1 |
-56.6 |
-44.3 |
-47.3 |
|
3.05 |
-52.8 |
-48.9 |
-18.5 |
-40.6 |
-24.2 |
-26.2 |
|
Mean |
-50 |
-39.1 |
-30.7 |
-61.3 |
-110.5 |
-114.5 |
|
9.77 |
-61.3 |
-101.1 |
-84.6 |
-74.5 |
-75.3 |
-91 |
|
Mean |
-65.7 |
-95.6 |
-96.7 |
-59.4 |
-115.2 |
-76.8 |
|
31.3 |
-78.3 |
-106.2 |
-98.2 |
-67 |
-111.1 |
-101 |
|
Mean |
-68.2 |
-110.8 |
-92 |
-34 |
-109.9 |
-104.6 |
|
100 |
-32.1 |
-113.9 |
-127.2 |
-29.2 |
-106.8 |
-99.9 |
|
Mean |
-31.8 |
-110.2 |
-110.6 |
Table 5. pH measurements during test
Conc. |
t = 0 h |
t = 72 h |
[mg/L] |
||
Control |
7.23 |
8.12 |
0.954 |
7.25 |
8.10 |
3.05 |
7.27 |
8.05 |
9.77 |
7.26 |
8.05 |
31.3 |
7.27 |
8.13 |
100 |
7.28 |
8.34 |
Table 6. Temperature during the test
Time |
Temperature[°C] min |
Temperature[°C] max |
[h] |
||
0 |
23.0 |
|
24 |
22.6 |
23.1 |
48 |
22.6 |
23.1 |
72 |
22.6 |
23.1 |
Table 7. Control, growth rates
Growth rates, µ [d-1] |
|||
0 h – 24 h |
0 h – 48 h |
0 h – 72 h |
|
0.97273 |
1.30075 |
1.38780 |
|
1.09861 |
1.37480 |
1.32503 |
|
Control |
0.95825 |
1.22658 |
1.28389 |
0.95266 |
1.17215 |
1.31350 |
|
1.27629 |
1.26036 |
1.34178 |
|
1.19280 |
1.34969 |
1.32424 |
|
Mean |
1.07522 |
1.28072 |
1.32937 |
Std. Dev. |
0.13712 |
0.07634 |
0.03445 |
CV [%] |
12.8 |
6.0 |
2.6 |
Std.Dev.:Standard deviation
CV: Coefficient of variation
Description of key information
Read-across: OECD 201, GLP, P. subcapitata, NOEC = 100 mg/L, EC50 > 100 mg/L (nominal)
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
This study was conducted in accordance with the OECD guideline 201; “Freshwater Alga and Cyanobacteria, Growth Inhibition Test" (28 July 2011), in order to determine the effects of the test item on the growth and yield of green alga Pseudokirchneriella subcapitata. Initial target cell densities of 0.5 × 104 cells/mL were employed for the individual replicates. The increase of cell numbers was assessed over a test period of 72 hours. A static test with nominal test item concentrations of 100, 31.3, 9.77, 3.05, 0.954 mg/L and control was performed. Six replicates were employed for the control and three for each test item concentration. The test was performed in 100 mL Erlenmeyer flasks each containing ~ 50 mL test solution. Analytical samples were analysed from control and test item concentration 100 mg/L at 0 hours (initial value) from fresh test solutions and after 72 hours from aged solutions. The measured initial concentration of the test item was 91.2 % of nominal in the highest test item concentration of 100 mg/L. In the aged samples the measured concentrations was 102.6 %.
No statistically significant inhibitory effects on any parameter (growth rate, yield) were observed at any test item concentration up to the highest concentration tested (100 mg/L, nominal) at test end. No abnormalities in the cells were observed at test end.
The overall LOEC was not determinable and the overall NOEC was observed to be 100 mg/L (nominal). The EC10, EC20 and EC50 for growth rate and yield were considered to be > 100 mg/L (nominal).
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