Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 222-426-8 | CAS number: 3468-11-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016-11-09 to 2016-12-09
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- July 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: municipal wastewater treatment plant Breisgauer Bucht Germany, 600,000 inhabitant equivalents, predominantly domestic wastewater.
- Concentration of sludge: 4.2 g/L after drying at 105°C
- Initial cell/biomass concentration: 30 mg/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- ca. 19.9 - ca. 21 mg/L
- Based on:
- TOC
- Remarks:
- calculated based on molecular structure
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium according to guideline
- Additional substrate: no
- Solubilising agent: no
- Test temperature: 20.5 - 23.1°C
- pH: not registered
- pH adjusted: no
- Aeration of dilution water: 2.5 to 4.3 bubbles/second or 30-100 mL/min with CO2-free air
- Suspended solids concentration: 30 mg dry solids/L
- Continuous darkness: no, diffuse light
TEST SYSTEM
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: aeration with CO2-free air
- Measuring equipment: Total organic carbon analyser TOC-5050A with autosampler ASI 5000A, Shimadzu.
- Details of trap for CO2 and volatile organics if used: two 250 mL glass wash bottles in series each filled with 200 mL 0.2 M NaOH, sampling through lateral connecting pieces, through butyl rubber septum using 5 mL PE syringes
SAMPLING
- Sampling frequency: on day 4, 7, 11, 14, 21 and 28
- Sampling method: 4 mL of NaOH sampled from 1st CO2-absorber flasks
- Sample storage before analysis: samples immediately closed with sealing film
- Final sampling: on day 28, 2mL of 4M HCl is added to the reactor to release CO2 dissolved in water. IC-analysis on day 29.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 3 replicates
- Abiotic sterile control: no
- Toxicity control: yes, 1 replicate at 40.1 mg/L TOC
- Reference substance: 3 replicates at 30 mg/L
- Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 45.9
- St. dev.:
- 7.57
- Sampling time:
- 29 d
- Remarks on result:
- other: as measured after acidification of the reactor flasks to release CO2 dissolved in water.
- Remarks:
- mean of 2 replicates
- Details on results:
- The 10-day window started rapidly, between day 0 and 4, but the biodegradation stagnated at a plateau between 40 and 50 % . As the maximal degradation, 55.2 %, did not exceed the pass level of 60 % it is concluded that the substance is not readily biodegradable. Details on the test results are provided below.
The 3rd replicate containing test item showed remarkable lower degradation, over the whole test duration. This is most probably caused by a leakage of the test reactor. - Results with reference substance:
- The reference substance was rapidly biodegraded, with maximal degradation of 87.1%.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The biodegradability of the test item was examined in a CO2-evolution test according to OECD 301B. The test item was found to be not readily biodegradable with on average 45.9 % biodegradation after 28 days.
- Executive summary:
The biodegradability of the test item was examined in a CO2-evolution test according to OECD 301B. After 28 days, the ultimate biodegradation was found to be 45.9 % (average of 2 replicates). The 10 -day window criteria was not met and the test item should be considered to be not readily biodegradable. The 3rd replicate was not considered for the assessment of the biodegradability of the test item. The degradation in this vessel was remarkably lower than in the other test vesels. This is most probably caused by a leakage in the test reactor.
Up to 72.6 % degradation was observed in the toxicity control containing sodium benzoate and the test item. This result shows that the test item was not inhibitory to the inoculum.
Reference
Ultimate biodegradation as % of ThCO2
Reactor | day 0 | day 4 | day 7 | day 11 | day 14 | day 21 | day 28 | day 29 |
Test item, rep 1 | 0 | 20.4 | 35.3 | 49.9 | 47.9 | 51.3 | 55.2 | 51.2 |
Test item, rep 2 | 0 | 20.0 | 27.1 | 40.5 | 34.2 | 42.4 | 45.6 | 40.5 |
Test item, rep 3 | 0 | 6.0 | 6.4 | 14.0 | 10.5 | 13.5 | 14.6 | 13.2 |
Reference, rep 1 | 0 | 71.0 | 80.8 | 79.2 | 83.0 | 77.1 | 82.4 | 84.0 |
Reference, rep 2 | 0 | 77.6 | 79.1 | 80.5 | 85.0 | 80.2 | 86.0 | 87.1 |
Reference, rep 3 | 0 | 68.5 | 77.6 | 77.1 | 84.1 | 76.7 | 82.6 | 84.6 |
Toxicity control | 0 | 46.7 | 59.2 | 68.4 | 71.5 | 68.4 | 72.2 | 72.6 |
The test results obtained for Test item, rep 3 are not taken into consideration. The laboratory reported that most likely the bottle leaked during the experiment, resulting in erroneous test results.
Description of key information
The biodegradability of the test item was examined in a CO2-evolution test according to OECD 301B. The degradation started quickly, but reached plateau after 11 days. After 28 days, the ultimate biodegradation was found to be 45.9 % (average of 2 replicates). The 10 -day window criteria was not met and the test item should be considered to be not readily biodegradable.
Up to 72.6 % degradation was observed in the toxicity control containing sodium benzoate and the test item. This result shows that the test item was not inhibitory to the inoculum.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
The 3rd replicate was not considered for the assessment of the biodegradability of the test item. The degradation in this vessel was remarkably lower than in the other test vesels. This is most probably caused by a leakage in the test reactor.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.