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EC number: 234-514-3 | CAS number: 12007-60-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Samples for possible analysis were taken from the test concentration and the control
Frequency at t=0 h and t=96 h
Volume 4.8 mL from the approximate centre of the test vessels.
Storage Samples were stored in a freezer (=-15°C) until analysis at the analytical laboratory of the Test Facility.
Additionally, reserve samples of 4.8 mL were taken from all test solutions for possible analysis. If not used, these samples were stored in a freezer (=-15°C) for a maximum of three months after delivery of the draft report, pending on the decision of the sponsor for additional analysis. - Vehicle:
- no
- Details on test solutions:
- The item was completely soluble in test medium at the concentrations tested therefore no correction was made for the purity/composition of the test item. Test solutions were prepared starting with the highest concentration of 100 mg/L. A one-day period of magnetic stirring was applied to accelerate dissolution of the test item in medium. The pH of the stock solution used for the limit test was adjusted with 1 M HCL (Merck, Darmstadt, Germany) from 9.2 to 8.9. Lower test concentrations were prepared by subsequent dilutions of the highest concentration in test medium. All test solutions were clear and colorless at the end of the preparation procedure.
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- Species Carp (Cyprinus carpio, Teleostei, Cyprinidae) Linnaeus, 1758
Source Zodiac, proefacc, "De Haar Vissen", Wageningen University and Research Centre, The Netherlands.
Mean length Range-finding test: 3.0 ± 0.3 cm
Limit test: 2.8 ± 0.3 cm
Mean weight Range-finding test: 0.40 ± 0.09 g
Limit test: 0.30 ± 0.14 g
Characteristics F1 from a single parent-pair bred in UV-treated water.
Reason for selection This system has been selected as an internationally accepted species.
Total fish used 26
The fish were held in quarantine for at least 12 days after delivery. In the holding tank, the adjusted ISO medium, formulated using RO-water (tap-water purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands) had the following composition:
CaCl2.2H2O 211.5 mg/L
MgSO4.7H2O 88.8 mg/L
NaHCO3 46.7 mg/L
KCl 4.2 mg/L
The fish were fed daily with pelleted fish food (Essence (300-500 um), Coppens International bv, Helmond, The Netherlands).
For batch to be valid, mortality during the seven days prior to the start of the test had to be less than 5%. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- Not stated
- Test temperature:
- temperature 20-24°C, constant within 2°C
- pH:
- The pH was 8.6 and 9.0 for the concentrations of 10 and 100 mg/L in the range-finding test, respectively, and 8.9 in the limit test.
- Dissolved oxygen:
- oxygen > 60% of air saturation
- Salinity:
- Not stated
- Conductivity:
- Not stated
- Nominal and measured concentrations:
- Samples taken from the limit concentration were analysed. The actual concentrations were at the level of 96-98% of nominal during the exposure period. Based on these results, the effect parameters were expressed in terms of analytically confirmed nominal concentrations.
- Details on test conditions:
- TEST SYSTEM
Test vessels: 6.5 litres, all-glass, containing 5 litres of test solution
Test medium: Adjusted ISO medium with a hardness of 180 mg CaCO3 per litre and a pH of 7.7 ± 0.3.
Number of fish: 7 each in the control and the limit concentration
Loading: 0.42 g fish/Litre, i.e. 7 fish per 5 litres of test medium
Illumination: 16 hours photoperiod daily
Aeration: Aeration was introduced after 56 hours of exposure.
Feeding: No feeding from 24 hours prior to the test and during the total test period
Introduction of fish: Within 7 minutes after preparation of the test media from a holding tank with comparable water quality parameters and pH and temperature differences between test and holding tank media of less than 0.5 unit and 1.0°C.
TEST MEDIUM / WATER PARAMETERS
Adjusted ISO medium, formulated using RO-water (tap-water purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands) with the following composition:
CaCl2.2H2O 211.5 mg/L
MgSO4.7H2O 88.8 mg/L
NaHCO3 46.7 mg/L
KCl 4.2 mg/L
TEST CONCENTRATIONS
- In the range-finding test, three fish per concentration were exposed to a range of 0.10 to 100 mg/L increasing by a factor of 10.
- In the limit test, the test concentration used was 100 mg/L. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- RANGE-FINDING TEST
No mortality or clinical effects were observed at any of the test concentrations during the test period. In the two highest concentrations precipitate was observed from the 24 hour of exposure.
Samples taken from nominal 100 mg/L were analysed. The initial concentration measured was 101 mg/L. This concentration remained stable during the test period (98% of initial). Therefore, the expected LC50 was above the nominal concentration of 100 mg/L.
LIMIT TEST
At the start of the limit test, analysis of the highest concentration solution was measured to be 98 mg/L. After 96 hours of exposure, the test item concentration was still the same at 98 mg/L. Based on this analysis, the effect parameters can be expressed in terms of analytically confirmed nominal concentrations.
As in the range-finding test, no mortality or other clinical effects were observed in both the control and the limit concentration throughout the test duration.
The 96h-LC50 for Cyprinus carpio exposed to dilithium tetraborate therefore exceeded the analytically confirmed nominal concentration of 100 mg/L. - Sublethal observations / clinical signs:
Table 1: Incidence of Mortality and Total Mortality During the Range-Finding Test
Dilithium Tetraborate
Nominal concentration (mg/L)Initial
number
of fishCumulative mortality
Total
Mortality
(%)2h
24h
48h
72h
96h
0.10
3
0
0
0
0
0
0
1.0
3
0
0
0
0
0
0
10
3
0
0
0
0
0
0
100
3
0
0
0
0
0
0
Table 2: Incidence of Mortality and Total Mortality During the Limit Test
Dilithium Tetraborate
Nominal concentration (mg/L)
Initial
Number
of fishCumulative mortality
Total
Mortality
(%)2½h
24h
48h
72h
96h
Control
7
0
0
0
0
0
0
100
7
0
0
0
0
0
0
- Validity criteria fulfilled:
- yes
- Conclusions:
- In conclusion, the 96h-LC50 for Cyprinus carpio exposed to dilithium tetraborate exceeded the analytically confirmed nominal concentration of 100 mg/L.
- Executive summary:
The objective of the study was to evaluate dilithium tetraborate for its ability to generate acute toxic effects in Cyprinus carpio during an exposure period of 96 hours and, if possible, to determine the LC50at all observation times. The study was conducted according to OECD guideline No. 203, 1992.
A limit test was performed based on the results of a preceding range-finding test. Seven fish per group were exposed to an untreated control and to a limit concentration of nominal 100 mg/L. The total exposure period was 96 hours and samples for analytical confirmation of exposure concentrations were taken at the start and at the end of the test.
The 96h-LC50 for Cyprinus carpio exposed to dilithium tetraborate exceeded the analytically confirmed nominal concentration of 100 mg/L.
Samples taken from the limit concentration were analysed. The actual concentrations were at the level of 96-98% of nominal during the exposure period. Based on these results, the effect parameters were expressed in terms of analytically confirmed nominal concentrations.
No mortality or other clinical effects were observed in this study.
The study met the acceptability criteria prescribed by the study plan and was considered valid.
In conclusion,the 96h-LC50for Cyprinus carpio exposed to Dilithium tetraborate >100 mg/L.
Reference
Description of key information
A limit test was performed based on the results of a preceding range-finding test. Seven fish per group were exposed to an untreated control and to a limit concentration of nominal 100 mg/L. The total exposure period was 96 hours and samples for analytical confirmation of exposure concentrations were taken at the start and at the end of the test.
Samples taken from the limit concentration were analysed. The actual concentrations were at the level of 96-98% of nominal during the exposure period. Based on these results, the effect parameters were expressed in terms of analytically confirmed nominal concentrations.
No mortality or other clinical effects were observed in this study.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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