Dimethyl[4-[4-(dimethylamino)-α-phenylbenzylidene]-2,5-cyclohexadien-1-ylidene]ammonium hydrogen sulphate

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Version / remarks:

04-04-1984

Principles of method if other than guideline:

SOP 4.006
The guideline was named "Daphnia sp., Acute Immobilisation Test and Reproduction Test" Part II - the reproduction test at the time.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Methanaminium, N-[4-[[4-(dimethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-methyl-, ethanedioate

EC Number:

241-922-5

EC Name:

Methanaminium, N-[4-[[4-(dimethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-methyl-, ethanedioate

Cas Number:

18015-76-4

Molecular formula:

C25H26N2O4

IUPAC Name:

Methanaminium, N-[4-[[4-(dimethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-methyl-, ethanedioate

Test material form:

solid: particulate/powder

Details on test material:

Basic Green 4 Oxalate

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

A stock solution of nominal 100 mg/L was diluted to the final concentration. Dilution water is filtrated surface water of a gravel pit near Leverkusen.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: Federal Health Agency, Berlin, Germany
- Feeding during test
- Food type: green algae (D. subspicatus)
- Amount: 0.1 - 0.4 mL solution
- Frequency: daily

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: sieving

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Test temperature:

19 - 21°C

pH:

8.0 - 8.4

Dissolved oxygen:

7.7 - 9.2 mg/L

Nominal and measured concentrations:

Nominal: 0 (control), 0.0081, 0.026, 0.081, 0.26, 0.81 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: beaker
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass, h = 11.5 cm, diameter: 9.5 cm, 250 mL test medium
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 8
- No. of vessels per control (replicates): 8

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 light / 8 dark
- Light intensity: ca. 6000 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 3.1
- Range finding study
- Results used to determine the conditions for the definitive study: 48-h EC50 = 0.81 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

< 0.008 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks:

offspring/maternal animal

Effect concentrations 2

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.26 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks:

maternal

Details on results:

No mortality occurred in the negative control. However, the reproduction rate of 40 offsprings / maternal animal was not reached, so the test is not considered completely valid.

Any other information on results incl. tables

Reproduction rates (x offspring / maternal animal):

Control = 11.03 (considered as 100%)

0.0081 mg/L = 5.4 (49%)

0.026 mg/L = 6.4 (58%)

No offspring at 0.081, 0.26 and 0.81 mg/L due to 80 -100% maternal mortality.

Applicant's summary and conclusion

Validity criteria fulfilled:

no

Remarks:

The reproduction rate in the negative control was too low.

Conclusions:

The 21-d NOEC (reproduction) to Daphnia magna was found to be <0.0081 mg/L. The reproduction toxicity might be overestimated due to the low reproduction rate in the whole test (negative control, too). However, due to the low 48-h EC50 (mortality) in the range finding study and the high maternal mortality rate in the main test, the substance can be considered chronically very toxic to aquatic invertebrates.