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EC number: 262-980-8 | CAS number: 61788-49-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001-06-05 to 2001-07-30
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study from supporting substance (structural analogue)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- 24th - 25th March 1997
Test material
- Reference substance name:
- Lanolin Alcohols
- IUPAC Name:
- Lanolin Alcohols
Constituent 1
- Specific details on test material used for the study:
- TOC: 81.6 %
ThC02: 2.99 mg CO2/mg test item
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal sewage treatment plant, 0-31137 Hildesheim
- Laboratory culture: Not recorded
- Method of cultivation: Not recorded
- Storage conditions: Not recorded
- Storage length: Not recorded.
- Pretreatment:
The activated sludge is maintained in an aerobic condition by aeration for four hours and then homogenized with a mixer. The sludge is filtered and the filtrate (30 mL) is subsequently used to initiate inoculation.
- Concentration of sludge: 15 mg/L
- Colony forming units of the inoculum: 10E7 - 10E8 CFU/L
- Colony forming units in the test vessels: 10E5 - 10E6 CFU/L
- Water filtered: yes
- Type and size of filter used, if any: not stated - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 15 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST SYSTEM
The following test solutions were prepared in 5 L brown glass bottles as incubation vessels:
• two incubation vessels for the test item concentration (P1, P2 )
• one incubation vessel for the reference item (R1)
• two incubation vessels for the inoculum control (C1, C2 )
• one incubation vessel for the toxicity control (T1)
The necessary amounts of aqua bidest., nutrient media and inoculum were placed in each of the incubation vessel. The vessels were then connected to the system for the production of CO2 free air and aerated for 24 h.
After 24 h the CO2 adsorption vessels were connected to the air outlets of the incubation vessels via a series of 3 gas wash bottles. The test and reference item concentrations were placed into the incubation vessels, the vessels made up to 3 L with CO2 free aqua bidest. and connected to the system for the production of CO2 free air.
Incubation took place in a temperature range between 22 ± 2 °C in a water bath. All vessels were stirred continuously throughout the test. On the 28th day 1 mL concentrated HCI was added to each of the vessels.
Aeration was continued for a further 24 h and on the 29th day the quantity of CO2 released in the last two gas wash bottles was determined.
- Measuring equipment:
pH-Meter, CORNING pH 240
Thermohygrograph, LAMBRECHT Tvp 3.015/3 K
Flow meter, KROHNE DUISBURG Tvp DK 800 PV
- CO2 analysis:
The total amount of CO2 produced in 28 days was analysed by titration in 12 measurements. Back titration of the residual Ba(OHh with 0.05 N HCI was carried out three times a week during the first ten days and thereafter twice weekly. On the 28th day the pH of all solutions were measured prior to acidification.
EVALUATION
The theoretical production of carbon dioxide (ThC02 ) of the test item and functional control is calculated by the carbon content (2) and the sum formula (1), respectively.
ThCO2 [mgCO2/mg] = (C-atoms x molecular weight of CO2)/molecular weight of test or reference item (1)
ThC02 [mgC02/mg] = 3.67 x TOC [mgC/mg] (2)
0.05 N HCI titrated had been converted into mg of CO2 produced:
1 mL HCI =1.1 mg CO2 (3)
The amount of CO2 produced is calculated by correcting the results of the test item and functional control for endogenous CO2 production of the control groups.
The biodegradation is calculated from the ratio theoretical CO2 production to net CO2 production in the following equation (4):
Degradation [%] = [Net CO 2 - (production x 100)]/ ThCO 2 [mgCO 2 /3L] (4)
Validity Criteria
The study was performed according to OECD 301 BI C02 Evolution Test and GLP Guidelines.
The quality criteria were fulfilled according to the guideline:
• The total CO2 production in the blank at the end of the test was less than 40 mg/L.
• The percentage degradation of the functional control reached the pass level of ≥ 60 % by day 14.
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
- Preliminary study:
- None conducted.
- Test performance:
- Not performed.
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 39
- Sampling time:
- 28 d
- Details on results:
- The 10 % level (beginning of biodegradation) was reached after a adaptation period of 9 days. In the 10-day-window the mean biodegradation came to 21 %. The pass level of a biodegradation > 60 % was not reached.
The test item must be regarded to be not readily biodegradable in the 10-day-window and after 28 days.
BOD5 / COD results
- Results with reference substance:
- In the control group a maximum of 36.3 mg CO2/L was formed after 28 days (quality criterion: < 40 mg CO2/L after 28 days).
In order to check the activity of the test system sodium acetate was used as functional control. The percentage degradation of the functional control reached the pass level of > 60 % after 8 days. After 14 days a degradation rate of 69 % was reached. The validity criterion of the guideline is fulfilled.
Any other information on results incl. tables
Table: Biodegradation of the test item in comparison to the functional control and toxicity control:
Biodegradation [%] |
||||
|
Day 6 |
Day 14 |
Day 21 |
Day 28 |
Test Item 15 mg/L |
5 |
16 |
23 |
39 |
Functional Control 35 mg/L |
51 |
69 |
75 |
69 |
Toxicity Control 15 mg/L test item + 35 mg/L reference item |
26
|
49 |
46 |
50 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test material attained 39 % degradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No 301B.
- Executive summary:
The ready biodegradability was determined with a non-adapted activated sludge for the test item over a test period of 28 days in the Modified Sturm Test. The study was conducted from 2001-06-07 to 2001-07-06 according to OECD 301 B/C02 evolution test in the DR.U.NOACK-LABORATORIUM FÜR ANGEWANDTE BIOLOGIE.
The test item was tested in a concentration of 15 mg/L in duplicates, corresponding to a carbon content (TOC) of 12.2 mgC/L in the test vessels.
The biodegradation of the test item was followed by titrimetric analyses of the quantity of CO2, which was produced by the respiration of bacteria. The degradation was finished on day 28 by acidification, the last titration was made on day 29, after the soluble CO2was turned out over a period of 24 h.
The CO2production was calculated as the percentage of total CO2that the test item could have theoretically produced based on carbon content. Biodegradation is therefore expressed as percentage ThC02and was calculated for each titration of CO2.
In order to check the activity of the test system sodium acetate was used as functional control. The percentage degradation of the functional control reached the pass level of > 60 % after 8 days. After 14 days a degradation rate of 69 % was reached. The validity criterion of the guideline is fulfilled.
In the toxicity control containing both test and reference item a biodegradation rate of 40 % occurred within 14 days. The biodegradation of the reference item seemed not to be inhibited by the test item in the toxicity control.
The 10 % level (beginning of biodegradation) was reached after a adaptation period of 9 days. In the 10 -day-window the mean biodegradation came only to 21 %. The pass level of a biodegradation > 60 % was not reached neither in the 10 -day-window nor after 28 days.
The test item must be regarded to be not readily biodegradable in the 10-day-window and after 28 days. The validity criteria according to the guideline are fulfilled.
Table:Biodegradation of the test item in comparison to the functional control and toxicity control
Biodegradation [%]
Day 6
Day 14
Day 21
Day 28
Test Item
15 mg/L
5
16
23
39
Functional Control 35 mg/L
51
69
75
69
Toxicity Control
15 mg/L test item + 35 mg/L reference item
26
49
46
50
Conclusion:
The test material attained 39 % degradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No 301B.
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