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EC number: 202-009-7 | CAS number: 90-66-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 22, 1979 to July 23, 1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in accordance with acceptable scientific methods.
- Qualifier:
- according to guideline
- Guideline:
- other: Based on Bioconcentration test of chemicals in fish and shellfish (Kanpogyo No. 5, Yakuhatsu No. 615, 49 Kikyoku No. 392)
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable - Radiolabelling:
- no
- Details on sampling:
- Test water:
No 1 concentration area:
Sampling of water 100 mL, addition of chloroform 50 mL (repeated twice)
Shaking
Dehydration filtration
Chloroform phase: drying, Addition of benzene-hexane (1:1 v/v) 5mL, column chromatography (see below for conditions)
Elution fraction: Drying, Quantification, 10mL (acetonitrile)
No 2 concentration area:
Sampling of water 1000 mL, addition of chloroform 100 mL (repeated twice)
Shaking
Dehydration filtration
Chloroform phase: drying, Addition of benzene-hexane (1:1 v/v) 5mL, column chromatography (see below for conditions)
Elution fraction: Drying, Quantification, 10mL (acetonitrile)
Fish:
- Measurement of body weight and length
- Fragmentation
- Addition of the sea sand, 10 g
- Addition of anhydrous sodium sulfate, 100 g
- Dehydration
- Addition of acetonitrile, 150 mL on 1st addition, 100 mL on 2nd addition, 100 mL on 3rd addition (repeated 3 times)
- Shaking (repeated 3 times)
- Vacuum filtration (repeated 3 times)
Filtrate: Addition of hexane, 100 mL and Shaking (repeated twice)
Acetonitrile phase: Drying, Addition of benzene-hexane (3:7 v/v) 5mL, Column chromatography method ( see below)
Elution fraction: Drying, Quantification, 10mL (acetonitrile) - Vehicle:
- yes
- Details on preparation of test solutions, spiked fish food or sediment:
- Dissolution method (dispersant and dispersion method)
Dispersant: Hydrogenated castor oil (HCO-20)
Dissolution (dispersion) method:
1 g of the test substance and 10 g of hydrogenated castor oil (HCO-20) were dissolved in 50 mL of acetone. Acetone was evaporated by rotary evaporator. Subsequently, water was added to quantify the constant volume of 1 L to prepare 1000 ppm (w/v) of the stock solution. - Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- Test fish
Carp: Mean body weight: 27.1 g, Mean body length: 10.3 cm
External disinfection and acclimatization
(1) External disinfection
Medicated bathing for 24 hours was conducted with 10 ppm of aqueous chlortetracycline hydrochloride under static water condition.
(2) Acclimatization
25 degreesC x 21 days - Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- not specified
- Remarks:
- freshwater
- Total exposure / uptake duration:
- 1 008 h
- Hardness:
- no data
- Test temperature:
- 25 +/ 2 degrees C
- pH:
- no data
- Dissolved oxygen:
- no data
- TOC:
- no data
- Salinity:
- not applicable
- Details on test conditions:
- Test conditions:
(a) Aquatic environment control equipment: Flow-through system
Test vessel: Glass vessel, Volume 100 L, Flow rate 576 L/day
(Stock solution: dilution water = 4 mL/minute : 400 mL/minute)
(b) Test fish
Carp Mean body weight: 27.1 g Mean body length: 10.3 cm
(c) External disinfection and acclimatization
(1) External disinfection
Medicated bathing for 24 hours was conducted with 10 ppm of aqueous chlortetracycline hydrochloride under static water condition.
(2) Acclimatization
25 degrees C x 21 days
(d) Dissolution method
1 g of the test substance and 10 g of hydrogenated castor oil (HCO-20) were dissolved in 50 mL of acetone. Acetone was evaporated by rotary evaporator. Subsequently, water was added to quantify the constant volume of 1 L to prepare 1000 ppm (w/v) of the stock solution.
(e) Test temperature
25 ± 2 degrees C - Nominal and measured concentrations:
- Concentration for the vessel
Reason for the selection of the concentration
The concentration which provides accurate quantification is approximately 2 ppm (see Figure 4). Recovery rate was 82 % at 100 fold concentration in pre-treatment on analysis of water. Based on the result of preliminary breeding for 10 days, decrease of concentration in the vessel was assumed as 10% and the concentration in the vessel for No.2 Concentration area was set at 0.03 ppm. The concentration of No.1 Concentration area was set at 10 fold of the concentration of No.2 Concentration area.
(Calculation formula)
Concentration in the vessel for No.2 Concentration area was calculated as follows: (2/100 x 82/100 x 90/100)/0.03 ppm)
Nominal value
No.1 Concentration Area: test substance 300 ppb w/v, dispersant 3000 ppb w/v
No.2 Concentration Area: test substance 30 ppb w/v, dispersant 300 ppb w/v
Measured value
Mean concentration for calculation of concentration rate
No.1 Concentration Area: 2W: 290, 3W: 287, 4W: 293, 6W: 298 ppb w/v
No.2 Concentration Area: 2W: 29.0, 3W:28.8, 4W: 26.7, 6W: 27.2 ppb w/v - Reference substance (positive control):
- no
- Type:
- BCF
- Value:
- 0.12 - <= 4.2
- Remarks on result:
- other: Conc.in environment / dose:300 ppb
- Type:
- BCF
- Value:
- 1.3 - <= 11
- Remarks on result:
- other: Conc.in environment / dose:30 ppb
- Details on kinetic parameters:
- No data available
- Metabolites:
- No data
- Results with reference substance (positive control):
- Not applicable
- Details on results:
- Condition of test fish
Result of external observation: normal
See tables below under any other information on results. - Validity criteria fulfilled:
- not specified
- Conclusions:
- The bioaccumulation study was conducted in 1979 and according to the method used in ‘Bioconcentration test of chemicals in fish and shellfish (Kanpogyo No. 5, Yakuhatsu No. 615, 49 Kikyoku No. 392’. A flow-through system was used and the fish tested were carp (mean body weight: 27.1 g and mean body length: 10.3 cm). Two concentrations of the substance were tested (30 and 300 ppb w/v) and a dispersant was used (hydrogenated castor oil). The condition of the test fish was considered to be normal throughout the study. At 30 ppb w/v, the BCF value ranged from 0.12 or below to 4.2. At 300 ppb w/v, the BCF value ranged from 1.3 to 11 .
- Executive summary:
The bioaccumulation study was conducted in 1979 and according to the method used in ‘Bioconcentration test of chemicals in fish and shellfish (Kanpogyo No. 5, Yakuhatsu No. 615, 49 Kikyoku No. 392’. A flow-through system was used and the fish tested were carp (mean body weight: 27.1 g and mean body length: 10.3 cm). Two concentrations of the substance were tested (30 and 300 ppb w/v) and a dispersant was used (hydrogenated castor oil). The condition of the test fish was considered to be normal throughout the study. At 30 ppb w/v, the BCF value ranged from 0.12 or below to 4.2. At 300 ppb w/v, the BCF value ranged from 1.3 to 11 . Based on these results, the test substance is not considered to be bioaccumulative.
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- April 2018
- Justification for type of information:
- see justification attached below
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Type:
- BCF
- Value:
- >= 0.12 - <= 4.2 dimensionless
- Remarks on result:
- other: Conc.in environment / dose:300 ppb
- Key result
- Type:
- BCF
- Value:
- >= 1.3 - <= 11 dimensionless
- Remarks on result:
- other: Conc.in environment / dose:30 ppb
- Validity criteria fulfilled:
- not applicable
- Remarks:
- Read across
- Conclusions:
- Based upon the data available, the substances are considered to be equivalent, except for the positioning of the functional groups, and are structural isomers of each other. The substances follow a regular pattern with regards to their chemical properties as a result of that structural similarity. Therefore, the definition of a chemical category has been met, and read across is considered appropriate for the category of chemical. The substance, CAS: 90-66-4; EC: 202-009-7, is analogous to the substance to be read across to, in terms of basic form, and the degree of substitution of functional groups is not considered to bias the effects for the endpoint of bioaccumulation. Steric hindrance is not considered a decisive factor in the case of this read across, and as such, it is considered that the substances would show similar bioaccumulation properties in the event of exposure. The substance is therefore considered to be not bioaccumulative for the defined endpoints on the basis of read across.
- Executive summary:
Based upon the data available, the substances are considered to be equivalent, except for the positioning of the functional groups, and are structural isomers of each other. The substances follow a regular pattern with regards to their chemical properties as a result of that structural similarity. Therefore, the definition of a chemical category has been met, and read across is considered appropriate for the category of chemical. The substance, CAS: 90-66-4; EC: 202-009-7, is analogous to the substance to be read across to, in terms of basic form, and the degree of substitution of functional groups is not considered to bias the effects for the endpoint of bioaccumulation. Steric hindrance is not considered a decisive factor in the case of this read across, and as such, it is considered that the substances would show similar bioaccumulation properties in the event of exposure. The substance is therefore considered to be not bioaccumulative for the defined endpoints on the basis of read across.
Referenceopen allclose all
Bioconcentration rate/factor of the test substance:
|
2W |
3W |
4W |
6W |
No.1 Concentration area |
3.2 |
0.12 or below |
(0.68) |
(2.0) |
0.12 or below |
(0.17) |
(1.2) |
4.2 |
|
No.2 Concentration area |
(1.1) |
(1.7) |
1.3 or below |
(5.7) |
1.3 or below |
1.3 or below |
1.3 or below |
(6.7) |
Referential values are shown in the parentheses.
The correlation of bioconcentration rate and quantification accuracy is shown below:
|
Concentration in fish body |
Bioconcentration rate |
Calculation method (ppm) |
Range of accurate quantification |
0.859 and above |
No.1 Concentration area: 2.9 and above No.2 Concentration area: 32 and above |
A C x D 100 E x F |
Range of referential value |
0.859-0.034 |
No.1 Concentration area: 0.12-2.9 No.2 Concentration area: 1.3-32 |
|
Range of detection limit |
0.034 or below |
No.1 Concentration area: 0.12 folds or below No.2 Concentration area: 1.3 folds or below |
B C x D 100 E x F |
Description of key information
LOWINOX TBP-6 is not bioaccumulative (BCF 1.3 -11).
Based upon the data available, the source and target substances are considered to be equivalent, except for the positioning of the functional groups, and are structural isomers of each other. The substances follow a regular pattern with regards to their chemical properties as a result of that structural similarity. Therefore, the definition of a chemical category has been met, and read across is considered appropriate for the category of chemical. The substance, CAS: 90-66-4; EC: 202-009-7, is analogous to the substance to be read across to, in terms of basic form, and the degree of substitution of functional groups is not considered to bias the effects for the endpoint of bioaccumulation. Steric hindrance is not considered a decisive factor in the case of this read across, and as such, it is considered that the substances would show similar bioaccumulation properties in the event of exposure. The substance is therefore considered to be not bioaccumulative for the defined endpoints on the basis of read across.
Key value for chemical safety assessment
- BCF (aquatic species):
- 11 dimensionless
Additional information
The key value for bioaccumulation has been derived via the use of a read across to 6,6'-di-tert-butyl-4,4'-thiodi-m-cresol; CAS: 96-69-5; EC: 202-525-2 in accordance with ANNEX XI (General rules for adaptation of the standard testing regime set out in annexes VII to X) of the REACH Regulation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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