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Diss Factsheets
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EC number: 258-658-1 | CAS number: 53605-94-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12 to 14 June 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted under the appropriate guideline following GLP methodology.
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Principles of method if other than guideline:
- not applicable
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
not applicable - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg/L (Limit Test) based on preliminary range-finding results
- Vehicle:
- no
- Details on test solutions:
- Test concentration were prepared by addition of the required amount of n-Butyl-3-hydroxybutyrate directly to the dilution water to give the required concentration of 100 mg/L.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: Smithers Viscient
- Age at study initiation (mean and range, SD): less than 24 hours
- Feeding during test: no
- Food type: suspension of Chlorella vulgaris
- Amount: 1 mg organic carbon/L of culture water and YCT
- Frequency: every other working day - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 20.4 to 21.6°C
- pH:
- 7.38 to 8.02
- Dissolved oxygen:
- 5.99 to 7.78
- Salinity:
- not applicable
- Nominal and measured concentrations:
- range finding nonmial concentrations were 0.1, 1.0, 10, and 100 mg/L; definitive nominal concentration was 100 mg/L reported as 95.8 mg/L measured or 96.3%.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: plastic
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 25 ml
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): 24 hour renewal
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 media:
- Range finding study yes
- Test concentrations: 0.1, 1.0, 10, 100
- Results used to determine the conditions for the definitive study: yes - Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- not specified
- Basis for effect:
- mortality
- Details on results:
- - Behavioural abnormalities: 5 daphnids in 100 mg/L were trapped in the meniscus at 24 hours with 1 daphnid moving slowly. At 48 hours, 6 organsims were trapped in the meniscus and 1 was moving slowly.
- Mortality of control: none
- Other adverse effects control: 2 organisms were trapped in the meniscus at 24 hours with no signs of abnormal behaviour at 48 hours
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- not applicable
- Reported statistics and error estimates:
- none
- Validity criteria fulfilled:
- yes
- Conclusions:
- The study was conducted as a Limit Test based upon preliminary range-finding results with no reported mortaliy in the 100 mg/L concentration. The study followed OECD 202 and GLP and was therefore deemed valid and reliable. The 48-hr EC50 value was > 100 mg/L (nominal).
- Executive summary:
A study was conducted to determine the the acute toxicity of n-Butyl-2 -hydroxybutyrate to the cladoceran, Daphnia magna in a 48 hour exposure following OECD 202. Analytical measurement of the exposure solutions showed mean measured concentrations that were 80 to 120% of nominal therefore all concentrations are reported as nominal concentrations. A preliminary range-finding study with concentrations of 0.1, 1.0, 10 and 100 mg/L had no mortality reported after 48 hours of exposure, so a limit test at 100 mg/L was conducted following guideline recommendations. The 48-hr EC50 value was estimated to be >100 mg/L. The highest no observed effect concentration (NOEC) for both mortality and visible sub-lethal effects after 96 hours was 100 mg/L.
Reference
Description of key information
The study was conducted as a Limit Test based upon preliminary range-finding results with no reported mortaliy in the 100 mg/L concentration. The study followed OECD 202 and GLP and was therefore deemed valid and reliable. The 48-hr EC50 value was > 100 mg/L (nominal).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
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