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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- From 11 March 1988 to 13 March 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Justification for type of information:
- Refer to the Quaternary ammonium salts (QAS) category or section 13 of IUCLID for details on the category justification. The study with the read across substance is considered sufficient to fulfil the information requirements as further explained in the provided endpoint summary.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on sampling:
- A clear stock solution of 3.03 mg/mL was prepared by diluting 0.0303 g of the test substance with distilled water in a 10 mL flask. The test solutions were prepared by adding the appropriate volume of stock solution to dilution water to total 1,000 mL. Each solution was mixed on a magnetic stirrer for 30 seconds and then divided into 4 beakers to provide replicate exposure treatments, each containing 200 mL. The remaining 200 mL of test solution were used for 0 h water quality measurement.
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Name of vehicle: Water
- Concentration of vehicle in test medium (stock solution): Stock solution of 3.03 mg/mL was prepared by diluting 0.0303 g of the test substance with distilled water in a 10 mL flask - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Species: Daphnia magna
- Source: Springborn Life sciences, Inc., Wareham, Massachusetts
- Age at study initiation (mean and range, SD): ≤24 h
- Feeding during test: No
ACCLIMATION
- Acclimation period: Cultures of the test organism over many generation has been continuously maintained at the lab
- Acclimation conditions (same as test or not): Yes
- Type and amount of food: Solution of green algae and yeast suspension daily
- Feeding frequency: Daily - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 180 mg/L as CaCO3
- Test temperature:
- 20-21⁰C
- pH:
- 7.9-8.3
- Dissolved oxygen:
- 98-99%
- Nominal and measured concentrations:
- 0, 236, 394, 667, 1,091, 1,818 and 3,030 µg/L as nominal concentration
- Details on test conditions:
- The test was conducted in 250 mL glass beaker. A clear stock solution of 3.03 mg/mL was prepared by diluting 0.0303 g of the test substance with distilled water in a 10 mL flask. The test solution was prepared by adding the appropiate volume of stock solution to dilution water to a total volume of 1 L. Each solution was mixed on a magnectic stirrer for 30 seconds and then divided into 4 beakers to provide replicate exposure treatments, each containing 200 mL. The remaining 200 mL of test solution was used for 0 hour water quality measurement and then discarded. One set of 4 control beakers was prepared containing the same dilution water and maintained under the same conditions as the exposure concentration, but without the test subsatnce. The ambient air temperature was controlled in order to maintain test solution temperature at 20 ± 1⁰C. The test solution was not aerated. The test area was illuminated with Sylvania GRO-LUX and cool white fluorescent lights at an intensity of 70 footcandles at the solution surface.
- Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 550 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 280 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: i.e., equivalent to 92.4 µg a.i. /L)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 236 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: mortality, behavioural and physical abnormalities
- Remarks on result:
- other: i.e., equivalent to 78 µg a.i. /L)
- Details on results:
- The 48 h EC50 and 95% confidence interval (calculated by probit analysis) was 280 (i.e. 92.4 ug a.i./L). The NOEC was <236 µg/L (<78 ug a.i./L), the lowest tested concentration.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the results of the read across study, the 48 h EC50 and NOEC of the test substance can also be considered to be 280 and <236 µg a.i./L (i.e., equivalent to 0.09 and <0.08 mg a.i./L), respectively.
- Executive summary:
A study was conducted to determine the acute toxicity study of the read across substance, Coco TMAC (33% active ingredient), to the Daphnia magna according to a method similar to OECD Guideline 202, in compliance with GLP. Daphnia magna (10 per dose) were exposed to the test substance at nominal concentrations of 0, 236, 394, 667, 1,091, 1,818 and 3,030 µg/L for 48 h under static conditions and mortalities were determined. The 48 h EC50and 95% confidence interval were calculated by probit analysis as 280 (250 - 300) µg/L. No analytical determination of the test substance was performed. The NOEC was < 236 µg/L, the lowest tested concentration. Based on the results, the 48 h EC50 and NOEC were 280 and <236 µg/L, (i.e., equivalent to 0.09 and <0.08 mg a.i./L), respectively (Surprenant, 1988). Based on the results of the read across study, the 48 h EC50 and NOEC of the test substance can also be considered to be 280 and <236 µg a.i./L (i.e., equivalent to 0.09 and <0.08 mg a.i./L), respectively.
Reference
Description of key information
Based on the results of the read across study, the 48 h EC50 and NOEC values of 280 and <236 µg a.i./L (i.e., equivalent to 0.09 and <0.08 mg a.i./L), respectively has been considered further for hazard assessment of C12-14 TMAC.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.09 mg/L
Additional information
A study was conducted to determine the acute toxicity study of the read across substance, Coco TMAC (33% active ingredient), to the Daphnia magna according to a method similar to OECD Guideline 202, in compliance with GLP. Daphnia magna (10 per dose) were exposed to the test substance at nominal concentrations of 0, 236, 394, 667, 1,091, 1,818 and 3,030 µg/L for 48 h under static conditions and mortalities were determined. The 48 h EC50and 95% confidence interval were calculated by probit analysis as 280 (250 - 300) µg/L. No analytical determination of the test substance was performed. The NOEC was < 236 µg/L, the lowest tested concentration. Based on the results, the 48 h EC50 and NOEC were 280 and <236 µg/L, (i.e., equivalent to 0.09 and <0.08 mg a.i./L), respectively (Surprenant, 1988). Based on the results of the read across study,the 48 h EC50 and NOEC of the test substance can also be considered to be 280 and <236 µg a.i./L (i.e., equivalent to 0.09 and <0.08 mg a.i./L), respectively.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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