3-dodecyl-1-(2,2,6,6-tetramethyl-4-piperidyl)pyrrolidine-2,5-dione

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

yes

Test organisms (species):

Cyprinus carpio

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

11,5 °dH

Test temperature:

mean = 20 °C

pH:

7.8 to 8.4

Details on test conditions:

Test tanks: 20 l
Test volume: 10 l, changed daily
Aeration: continuous in the test tanks
Light/dark cycle: 16/8 h
Density of animals: 10 animals per test tank, each at ~ 1.0 g
Oxygen values during the test: 89 - 100 % saturation

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.097 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

0.06 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

0.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

The LC50 (96 h) was determined to be 0.097 mg/L (range 0.06 mg/l - 0.13 mg/l).

Executive summary:

The acute fish toxicity of the substance was tested according to OECD Guideline 203 compliant with GLP (RL1; Evonik, 2000). Cyprinus carpio was exposed in a semi-static testing regime (daily medium exchange). Test item analysis (dissolved organic carbon) was performed for the stock solution (0 h and 96 h: the measured concentration of the stock solution after 96 h did not deviate by more than 20% from the freshly prepared solution) as well as for separately incubated stability samples (without fish) for three nominal concentrations (2 mg/L; 5 mg/L; 10 mg/L) after 0 hours and after 24 hours. The recovery rate of the additional concentration batches was more than 80%. The measured values after 24 h did not deviate by more than 20% from the freshly prepared solutions. As such, the test item was assumed to be stable in test medium during the test and all (no)effect concentrations were calculated based on nominal values. The nominal concentrations for the test were calculated based on the analytical value determined for the stock solution after 0 h.

10 fish were used per treatment level: 5 test item concentrations (1.0; 0.5; 0.25; 0.13; 0.06 mg/L) plus one control.

pH value during the test was between 7.8 and 8.4, oxygen saturation levels were between 89% to 100%.

The following (no)effect concentrations were determined:

LC50 (96 h) = 0.097 mg/L (range 0.06 mg/l - 0.13 mg/l);

LC0 (96 h) = 0.06 mg/L;

LC100 (96 h) = 0.25 mg/L.

Description of key information

In a semistatic test according to OECD 203 with daily renewal, the following (no)effect concentrations were determined for Cyprinus carpio:

LC50 (96 h) = 0.097 mg/L (range 0.06 mg/l - 0.13 mg/l);

LC0 (96 h) = 0.06 mg/L;

LC100 (96 h) = 0.25 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.097 mg/L

Additional information

The acute fish toxicity of the substance was tested according to OECD Guideline 203 compliant with GLP (RL1; Evonik, 2000). Cyprinus carpio was exposed in a semi-static testing regime (daily medium exchange). Test item analysis (dissolved organic carbon) was performed for the stock solution (0 h and 96 h: the measured concentration of the stock solution after 96 h did not deviate by more than 20% from the freshly prepared solution) as well as for separately incubated stability samples (without fish) for three nominal concentrations (2 mg/L; 5 mg/L; 10 mg/L) after 0 hours and after 24 hours. The recovery rate of the additional concentration batches was more than 80%. The measured values after 24 h did not deviate by more than 20% from the freshly prepared solutions. As such, the test item was assumed to be stable in test medium during the test and all (no)effect concentrations were calculated based on nominal values. The nominal concentrations for the test were calculated based on the analytical value determined for the stock solution after 0 h.

10 fish were used per treatment level: 5 test item concentrations (1.0; 0.5; 0.25; 0.13; 0.06 mg/L) plus one control.

pH value during the test was between 7.8 and 8.4, oxygen saturation levels were between 89% to 100%.

The following (no)effect concentrations were determined:

LC50 (96 h) = 0.097 mg/L (range 0.06 mg/l - 0.13 mg/l);

LC0 (96 h) = 0.06 mg/L;

LC100 (96 h) = 0.25 mg/L.