Sunflower oil, ester with sorbitol

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Version / remarks:

2006

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Sunflower oil, ester with sorbitol

EC Number:

306-248-9

EC Name:

Sunflower oil, ester with sorbitol

Cas Number:

96690-53-8

IUPAC Name:

sunflower seed oil sorbitol esters

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

Test organism: Desmodesmus subspicatus CHODAT SAG 86.81 (formerly Scenedesmus subspicatus CHODAT)

Reason for the selection of the test system: According to the guideline Desmodesmus subspicatus CHODAT SAG 86.81 is suitable for this kind of study.

Origin: Sammlung von Algenkulturen (SAG), Pflanzenphysiologisches lnstitut der Universität Göttingen, Nikolausberger Weg 18, 37073 Göttingen, Germany

Cultivation at test facility: Fresh stocks were prepared every month on Z-Agar. Light intensity amounted 35-70 µE.m-2.s-1 for 24 h per day.

Culture medium: Nutrient medium Z according to LOTTGE et al. (1994).

SELF FLUORESCENCE: No self fluorescence has been found at 100 mg/L.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

21-23°C ; mean 22°C

pH:

7.7-9.37

Nominal and measured concentrations:

nominal: 100 mg/l

Details on test conditions:

TEST METHOD: Static procedure

Exposure duration: 72 h

Replicates: Six replicates per limit concentration and control

Test container: Sterile Erlenmeyer flasks, volume: 250 ml, sealed with cotton wool plugs

Test volume: 100 ml

Dilution water: According to the guideline

Preculture: A three day old preculture was used as inoculum. Incubation was performed in 500 mL Erlenmeyer flasks with dilution water. For the start of the test the preculture was directly pipetted to the test and control replicates to receive an initial cell concentration of approximately 2 - 5 x 10^3 cells/mL in the replicates.

Application: Application was, carried out by adding appropriate volumes of the stock solution to the test replicates.

Temperature: Nominal range: 21 - 24 °C, controlled at± 2 °C

Agitation: Test containers were placed on a rotary shaker and oscillated at approximately 70 rpm.

Light intensity: 60 - 120 µE.m-2.s-1

Light regime: 24 h/d light

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

Cell density was measured daily via Chlorophyll-a- fluorescence, excitation at 436 nm, emission at 685 nm. Dilution water was used as background signal. The pH-value at the beginning of the test was measured out of one additional replicate of the limit concentration and control. At the end it was measured from one pool of all replicates of the limit concentration and control. The room temperature was measured continuously by a hygro-thermograph. Light intensity was measured prior to test start. Microscopic evaluation of the cells at the start and end of the incubation were carried out. The cells were checked for unusual cell shapes, colour differences, differences in chloroplast morphology, flocculation, adherence of algae to test containers and agglutination of alga cells.

Microscopic evaluation of the cells at start and end of the incubation period revealed no morphological abnormalities.

The environmental conditions (pH-value, room temperature, light intensity) were determined to be within the acceptable limits.

Results with reference substance (positive control):

The toxicity of potassium dichromate to the unicellular freshwater green alga Desmodesmus subspicatus was determined over a period of 72 h from March 09 to 12, 2009.

Test concentrations: 0.25 - 0.50 - 1.0 mg/L
ErC50= 0.695 mg/l (95%-CI: 0.657- 0.729 mg/l)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50-values for specific growth rate inhibition (ErC50) and inhibition of yield (EyC50) after 72 h were > 100 mg/L.

Executive summary:

The toxicity of the test item to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD 201 at DR.U.NOACK-LABORATORIEN in 31157 Sarstedt, Germany from April 24 to 27, 2009. The aim of the study was to assess the effects on growth rate and yield production over a period of 72 h in a limit test. The study was conducted under static conditions with an initial cell density of nominally 2 - 5 x 103 cells/ml. Six replicates were tested for the limit concentration of 100 mg/L and the control. Environmental conditions were determined to be within the acceptable limits. The concentration of the test item was analysed at test start via DOC-analysis. All effect levels are given based on the nominal concentration of the test item.

The EC50-values for specific growth rate inhibition (ErC50) and inhibition of yield (EyC50) after 72 h were > 100 mg/L.