Cobaltate(3-), bis[4-[4-[[4-[4-[[5-(aminosulfonyl)-2-hydroxyphenyl]azo]-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl]phenyl]azo]-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl]benzenesulfonato(3-)]-, trisodium

Short-term toxicity to aquatic invertebrates

Description of key information

EC₅₀ (48 h, Daphnia magna) = 38.33 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

38.33 mg/L

Additional information

The following data were obtained for the Similar Substance 01. It is expected that the Target Substance will present similar effects on aquatic invertebrates. Justification for Read Across is given in Section 13 of IUCLID.

A study was conducted to determine the acute toxicity of the test substance to Daphnia magna, according to the OECD Guideline 202 (2004) and the EU method C.2 of the Regulation EC 440/2008. The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L. For each test concentration, 20 Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted. Potassium dichromate K₂Cr₂O₇was used as positive control in a current reference study to assure that the test conditions are reliable. The measured concentrations were between 103 % and 105 % of the nominal concentration and the start concentration, and after 48 hours between 95 % and 111 % respectively. Therefore, the determination of the biological results was based on the nominal concentrations.

The results determined for the test item were:

48h-NOEC = 22.00 mg/L

48h-LOEC = 46.00 mg/L

24h-EC50 (or LC50) = 46.28 mg/L

48h-EC50 (or LC50) = 38.33 mg/L