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Reaction mass of Sodiumbis[1-[(2-hydroxy-5-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) and Sodium [1-[(2-hydroxy-4-nitrophenyl)azo]-2-naphtholato(2-)][1-[(2-hydroxy-5-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) and Sodiumbis[1-[(2-hydroxy-4-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-)
EC number: 915-756-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17 May 2016 to 31 May 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 2008
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Name of test substance (as cited in the study report): Eukesolar Black ER Liquid - dried
- BASF test substance number: 14/0317-1
- Batch No: Dye powder sample 14/103 from Material no. 52629782; batch no. M-R/G
- Identity: Confirmed
- Purity: 99.11 area-% (HPLC, 260 nm), 99.71 area-% (HPLC, 375 nm).
- Homogeneity: homogeneous
- Expiry date: 09 Jun 2017
- Date of production: 09 Jun 2014
- Appearance: Solid, black
- Water-solubility: The test item is a mixture and a defined water solubility was not provided. Undissolved components of the test item were visible up to 100 mg/L in test media (observation of the ecotoxicology laboratory).
- Storage conditions: The test substance was stored at ambient temperature under argon. - Analytical monitoring:
- no
- Remarks:
- No analytical method is available for the multi-constituent test substance. See 'Details on analytical methods' for further information.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test treatments were prepared separately for each test vessel. The test substance was weighed into a beaker. The defined volume of test water was filled into the test vessel. An aliquot from the test water in the test vessel was taken and mixed with the test substance using a high shear mixer (Ultra Turrax, IKA Werke GmbH &Co. KG, Germany) and the mixture was poured into the test vessel and the whole test batch was homogenized again with an ultra turrax. The test treatments were then stirred for about 2 days. Undissolved test substance was removed by filtration with a paper filter. The test solutions were filtered again with membrane filter (Whatman, pore width 0.2 μm, OE66). The first 50-100 mL of filtered solution was discarded (used to condition the filter). After filtering the solution was stirred for 10 min to ensure adequate dissolved oxygen levels. The control group was treated in the same way.
- Preparation scheme: see 'Any other information on materials and methods incl. tables'.
- Evidence of undissolved material: The control test treatment was clear. The 100 mg/L test treatment appeared clear and violet for up to 72h. At 72h it was turbid dark violet. After 96 hours the test treatment appeared turbid, dark violet and test substance was visible at the bottom of the test vessel. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: The fish were hatched at the testing facility.
- Body length: 3.9 cm (range 3.5 cm – 4.2 cm); Measurements were performed with all surviving fish from the control and lowest test concentration tanks at the end of exposure as a representative sample of the fish batch.
- Body weight: 0.48 g (range 0.30 g – 0.59 g); Measurements were performed with all surviving fish from the control and lowest test concentration tanks at the end of exposure as a representative sample of the fish batch.
- Age of the animals: Approx. 9 months
- Hatching date: 30 Aug - 01 Sept 2015
ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions: same as test
- Housing: Prior to testing, the batch of fish are housed in a glass tank (approx.45 L) receiving a continuous supply of fresh test water.
- Photoperiod: 16 hours light, 8 hours dark
- Water quality: The water used for housing and test purposes is nonchlorinated charcoal filtered drinking water (Frankenthal, Germany) mixed with deionized water and aerated to achieve the following water characteristics. The mixed test water is sanitized by UV treatment prior to entering the aquaria. Total hardness: Approx. 1 mmol/L = 100 mg/L CaCO3; Conductivity: Approx. 250 μS/cm (at 25°C); Ca content: Approx. 40 mg/L; Mg content: Approx. 5 mg/L; Acid capacity: Approx. 2.5 mmol/L; pH-value: Generally 7.5 – 8.5; Oxygen content: >80% saturation; TOC: Generally <2mg/L
- Temperature: 23 ± 1°C
- Diet: Commercial fish diet ”Tetramin“ (manufacturer Tetra-Werke, Melle, Germany) daily ad libitum, additionally generally on workdays live juvenile brine shrimp (Artemia nauplii).
- Withdrawal of feed: No feeding from approximately 24 hours before start of exposure
- Medical treatment: None during acclimatization
- Mortality during the last week before start of exposure: 0% - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 100 mg/L CaCO3
- Test temperature:
- 22 - 23 °C
- pH:
- 8.3 - 8.6
- Dissolved oxygen:
- 7.8 - 8.3 mg/L
- Conductivity:
- 250 μS/cm
- Nominal and measured concentrations:
- - Nominal loading rate: 0 (control) and 100 mg test substance/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Approx. 10 Liter stainless steel aquaria (29x21x22 cm)
- Type (delete if not applicable): open / closed
- Test volume: 10 L
- Aeration: Continuous slight aeration via glass capillary.
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 7 Fish/ test vessel, 0.34 g fish/L
- Randomization: The test organisms were introduced into the test vessels according to a randomization plan prepared by using a program of the laboratory data evaluation group of the testing facility.
- Feeding: none
TEST MEDIUM / WATER PARAMETERS
- Water: similar to the water used during acclimation period, see 'Details on test organisms'.
- Intervals of water quality measurement: The appearance of the test solutions and dissolution behavior of the test substance was recorded daily. Temperature, oxygen content and pH-value were measured in each test vessel within 1 hour after start of exposure and after 24, 48, 72 and 96 hours. Additionally the TOC (Total organic carbon) values of the test concentration and the control was analyzed after start and at the end of the exposure.
OTHER TEST CONDITIONS
- Adjustment of pH: No adjustment of the pH-value
- Photoperiod: 16h light, 8h dark
- Light intensity: 114 - 431 Lux (the light intensity is determined in regular intervals at the surface of the aquaria)
EFFECT PARAMETERS MEASURED
- Mortality and toxic signs: the fish were observed for mortality and toxic signs (changes appearance and abnormal behavior in comparison to the control group) within 1 hour after start of exposure and 6, 24, 48, 72 and 96 hours after start of exposure. Fish were considered dead if there was no visible movement and no reaction after touching.
- Body weight: At the end of exposure the body wet weight and total length of a representative sample of fish were determined. The representative sample consists of all surviving fish from the lowest concentration and control group.
TEST CONCENTRATIONS
- Results used to determine the conditions for the definitive study: In the first experiment mortality after 24 hours was observed, which may be due to physical effects, because undissolved test substance was not removed. - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- loading rate
- Basis for effect:
- mortality (fish)
- Details on results:
- MORTALITY
No deaths were observed throughout the test.
GENERAL SYMPTOMS OF TOXICITY
All fish displayed normal behavior. No other visual signs of toxicity were observed.
OBSERVATIONS ON TEST SOLUTIONS
The control test treatment was clear. The 100 mg/L test treatment appeared clear and violet for up to 72h. At 72h it was turbid dark violet. After 96 hours the test treatment appeared turbid, dark violet and test substance was visible at the bottom of the test vessel.
OTHER FINDINGS
- Water quality: TOC concentrations in the control at the start and end of the experiment were 2.4 mg/L and 1.0 mg/L, respectively. TOC concentrations in the 100 mg/L test substance loading rate at the start and end of the experiment were 16.0 mg/L and 13.8 mg/L, respectively. The higher TOC in the 100 mg/L treatment demonstrates that the test organisms were exposed to soluble organic components from the test substance. - Reported statistics and error estimates:
- Since the study is designed as a limit test, the data were not appropriate for the use of computerized methods for the calculation of point estimates. Any reported point estimates (NOEC and LCx values) are estimated based on expert judgment in accordance with test guideline recommendations. The test endpoints were calculated and reported based on the nominal test substance loading rate.
- Validity criteria fulfilled:
- yes
- Remarks:
- see 'Any other information on materials and methods incl. tables'.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 7 Nov 1988 to 11 Nov 1988
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: DIN 38 412 “ Testverfahren mit Wasserorganismen (Gruppe L). Allgemeine Hinweise zur Planung, Durchführung und Auswertung biologischer Testverfahren" und "Bestimmung der Wirkung von Wasserinhaltsstoffen auf Fische - Fischtest (L15)", June 1982
- Version / remarks:
- June, 1982
- GLP compliance:
- no
- Specific details on test material used for the study:
- Name of test material (as cited in study report): Eukesolarschwarz R-150 FLG
- BASF test substance number: 88/90
- Batch No.: 275
- Solubility in water: miscible in any ratio
- Physical state: liquid - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: In the form of an aqueous emulsion (10 g/L) the product was added to the test water; subsequently the fish were placed into the aquaria. - Test organisms (species):
- Leuciscus idus
- Details on test organisms:
- TEST ORGANISM
- Common name: Golden Orfe
- Strain: golden variety
- Source: Fischzucht Paul Eggers, D-2354 Hohenwestedt, FRG
- Body length: 7.0 cm (range: 6.0 - 8.5)
- Body weight: 4.4 g (range: 2.8 - 7.1)
- Corpulence factor of the batch: 1.3
HOUSING
- The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air.
- Photoperiod: 16 hours light and 8 hours dark
- Total hardness: about 2.5 mmol/L
- Acid capacity: about 5.5 mmol/L
- Oxygen content: >60% of maximum saturation
- pH: about 8.0
- Duration of housing and adaptation: about 2 months
- Adaptation to test water and test temperture: 3 days
- Water temperature: 19 - 22 °C during housing, 20°C during adaptation period and test
- Mortality during the last 2 weeks of housing: about 0.07%
- Mortality during the adaptation period: 0%
- Medical treatment: twice with 0.05 mg/L malachite green chloride; once with 10 mg/L tetracycline hydrochloride
- Diet: ad libitum growing feed F/B 50, SSNIFF Spezialdiaeten GmbH, D-4770 Soest, FRG
- Withdrawel of food before exposure: 1 day - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 2.5 mmol/L
- Test temperature:
- 20 °C
- pH:
- 7.4 - 7.6
- Dissolved oxygen:
- 8.1 - 8.9 mg/L
- Nominal and measured concentrations:
- - Nominal test item concentrations: 0 (control), 46.4, 100, 215 and 464 mg/L
- Details on test conditions:
- TEST SYSTEM
- Aeration: slight continuous aeration with oil-free air
- Volume of water: 10 liters
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Loading (g fish/ L test water): 4.4
- Test vessel: all-glass aquarium (30 cm x 22 cm x 24 cm)
TEST MEDIUM
- Source/preparation of dilution water: Reconstituted freshwater according to DIN 38 412, part 11,October 1982, preparation from fully demineralized tap water (conductivity max. 10 μMHO), resalting is carried out by addition of 294.0 mg/L CaCl2·2H2O, 123.3 mg/L MgSO4·7H2O, 63.0 mg/L NaHCO3 and 5.5 mg/L KCl.
WATER PARAMETERS OF TEST WATER READY FOR USE
- Total hardness: 2.5 mmol/L
- Acid capacity: 0.8 mmol/L
- Ratio Ca/Mg ions: 4 : 1
- Ratio Na/K ions: 10 : 1
- pH: about 8.0
OTHER TEST CONDITIONS
- Test temperature: 20 °C
- Photoperiod: 16 hours light and 8 hours dark
EFFECT PARAMETERS MEASURED:
- Survival and symptoms (at t = 1, 4, 24, 48, 72 and 96 h)
TEST CONCENTRATIONS
- Reason for selection of the concentrations: based on the results of range finding studies and main studies not evaluated (LC50 after 96 h: between 100 and 320 mg/L) - Reference substance (positive control):
- yes
- Remarks:
- Chloroacetamide
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 220 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- GENERAL OBSERVATION
The aquarium contents were intensely coloured and cloudy.
MORTALITY
No mortality was observed in the control, 46.4 and 100 mg/L test concentration. At the test concentration of 215 mg/L 2 fish (20%) were dead after 24 hours exposure and 4 fish (40%) were dead after 48 hours. At the highest tested concentration all fish had died after 24 hours exposure. See 'Any other information on results incl. tables'.
SYMPTOMS:
- Behavioural abnormalities: Apathy and narcotic-like state was observed at 215 mg/L after 24 hours; narcotic-like state was observed at 464 mg/L after 4 hours. Since the test solutions were intensely coloured and cloudy a complete evaluation of the symptoms could not be performed. - Results with reference substance (positive control):
- Chloroacetamide LC50 after 48 h: about 31 mg/L (determined 10 Oct. 1988), this lethal concentration corresponds to the normal sensitivity. This respective test was valid.
- Reported statistics and error estimates:
- Determination or calculation of the median lethal concentration (LC 50) and, if possible the LC 5 and the LC 95 using the probit analysis.
- Sublethal observations / clinical signs:
Table: Results on fish survival and symptoms
Nominal conc.
(mg/L)
Number of fish
Dead* fish after
1h
4h
24h
48h
72h
96h
46.4
10
0
0
0
0
0
0
100
10
0
0
0
0
0
0
215
10
0
0
2
4
4
4
464
10
0
0
10
10
10
10
0
10
0
0
0
0
0
0
Nominal conc.
(mg/L)
Symptoms*
1h
4h
24h
48h
72h
96h
46.4
100
215
an
464
n
0
Explanation of symptoms:
a=apathy, b=abdominal distension, e=exophthalmos,f=escapereflex, h=hyperreflexia,k=convulsions,l=gasping, n=narcotic-likestate,t=tumbling,u=restlessness,v=discoloration,w=headstand,x=accelerated respiration
* for checking for dead fish and for possible symptoms the fish were directed towards the front pane of the aquaria using a net.
Table: Effect values
LC50 (mg/L; nominal concentrations) after
1h
greater
460 (mg/L)
(1% significance level)
4h
greater
460 (mg/L)
(1% significance level)
24h
greater
220 (mg/L)
(10% significance level)
lower
460 (mg/L)
(1% significance level)
48h
about
220 (mg/L)
72h
about
220 (mg/L)
96h
about
220 (mg/L)
Symptoms: Since the test solutions were intensely coloured and cloudy a complete evaluation of the symptoms could not be performed.
No Observable Effect Concentration: 100 mg/l
Maximum concentration causing no mortality: 100 mg/l
Minimum concentration causing 100% mortality: 464 mg/l
- Validity criteria fulfilled:
- yes
- Remarks:
- see 'Any other information on materials and methods incl. tables'.
Referenceopen allclose all
Description of key information
The 96-h LL50 value is >100 mg/L (based on nominal test substance loading rate) in freshwater fish (Danio rerio).
Key value for chemical safety assessment
Additional information
The short-term toxicity of the substance to freshwater fish was determined in two studies; one with the dried substance and one with the liquid (non-dried) substance.
In the key study, the short-term toxicity of the dried substance to freshwater fish was determined in a limit test according to OECD TG 203 and in compliance with GLP criteria (BASF, 2017). In this study, groups of 7 zebrafish (Danio rerio) were exposed to nominal loading rates of 0 (control) and 100 mg test substance/L for 96 hours under static conditions. Test concentrations were not analytically verified because a reliable method for analysis in the required concentration range could not be developed. Since all reasonable efforts were taken to produce a WAF of the test substance in the test media following the guidance of OECD 23, results are based on the loading rate. The appearance of the test solutions and dissolution behavior of the test substance was recorded daily. Fish mortality and general symptoms of toxicity were recorded within 1 hour after start of exposure and after 6, 24, 48, 72 and 96 hours exposure. In this study the OECD validity criteria are met. The 100 mg/L test treatment appeared clear and violet for up to 72h. At 72h it was turbid dark violet. After 96 hours the test treatment appeared turbid, dark violet and test substance was visible at the bottom of the test vessel. No mortality or visual signs of toxicity were observed during the test. Based on these findings the 96-h LL50 value was determined at >100 mg/L.
This result is supported by data from a second study, where the short-term toxicity of the liquid substance to freshwater fish was determined according to DIN 38 412 (BASF, 1989). In this study, groups of 10 Golden Orfe (L. idus) were exposed to nominal test substance concentrations of 0 (control), 46.4, 100, 215 and 464 mg/L for 96 hours under static conditions. Test concentrations were not analytically verified. Incidences of mortality and clinical effects were recorded after 1, 4, 24, 48, 72 and 96 hours exposure. Since test solutions were intensely coloured and cloudy a complete evaluation of the symptoms could not be performed. Apathy and narcotic-like state was observed at 215 mg/L after 24 hours; narcotic-like state was observed at 464 mg/L after 4 hours. No mortality was observed in the control, 46.4 and 100 mg/L test concentration. At the test concentration of 215 mg/L 2 fish (20%) were dead after 24 hours exposure and 4 fish (40%) were dead after 48 hours. At the highest tested concentration all fish had died after 24 hours exposure. Based on these findings the 96-h LC50 is determined at ca. 220 mg/L. Even though non-dissolved test substance was not removed from the medium, meaning the substance could e.g. possibly clog or goat gills, this exposure was considered "worst-case", allowing the results to be used as supporting information.
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