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EC number: 206-986-0 | CAS number: 407-41-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2017-08-21 to 2017-09-13
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- TOXI-COOP ZRT., 8230 Balatonfüred, Arácsi út 97., Hungary
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: 4 replicate samples of 5 mL were taken from the test solution (100 mg/L) and the control at the start and the end of the test. Formulation samples were diluted with Ultrapure Water and analysed after derivatization by an HPLC method with UV detection.
- Sample storage conditions before analysis: Samples were tested immediately after sampling. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- The test solution used in the test was prepared by mechanical dispersion without using of any solubilising agent. An amount of 0.03 g test item was dissolved in 300 mL dilution water (ISO Medium) to obtain the nominal concentration of 100 mg/L. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Straus
- Source: Laboratory of Hydrobiology (Central Agricultural Office, Directorate of Plant-, and Soil Protection) 2100 Gödöllő, Kotlán S. u. 3., Hungary
- Feeding during test: no
ACCLIMATION
- Test animals were bred under similar conditions as that used during the exposure period, therefore additional acclimatisation before the test was not necessary. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 249 mg/L CaCO3
- Test temperature:
- 19.6 - 20.2 °C
- pH:
- 6.18 - 8.11
- Dissolved oxygen:
- 7.49 - 8.21 mg/L
- Salinity:
- NA
- Nominal and measured concentrations:
- Nominal concentrations: 0 and 100 mg/L
Measured concentration: in the range of 100 - 105 % of the nominal concentration - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers
- Fill volume: approximately 40 mL medium
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO Medium, prepared according to OECD 202
- Culture medium different from test medium: Reconstituted water of a similar quality regarding to pH, components of the main ions and total hardness as the test water used in the test.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16-hour light, 8-hour dark cycle
- Light intensity: 638 lux
RANGE-FINDING STUDY
- Test concentrations: 0 and 100 mg/L
- Results used to determine the conditions for the definitive study: No immobility or any sub-lethal effects were detected. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- There was no immobilisation observed in twenty daphnids exposed to either test item treated or control group. In addition to immobility, no abnormal behaviour or appearance of test animals was detected. Accordingly, the 48-h NOEC was determined to be 100 mg/L. The 48-h EC50 and LOEC were determined to be higher than 100 mg/L.
- Results with reference substance (positive control):
- The date of the last study with reference item Potassium dichromate was: 07 – 08 March 2017.
The 24h EC50: 1.41 mg/L, (95 % confidence limits: 1.19 – 1.68 mg/L) - Validity criteria fulfilled:
- yes
- Conclusions:
- In a short-term toxicity test with Daphnia magna, the test item showed no immobilisation or any other toxic effects up to the limit test concentration. Accordingly, the 48-h EC50 was determined to be >100 mg/L.
- Executive summary:
In the acute immobilisation test with Daphnia magna (STRAUS), the effects of the test item were determined according to OECD 202, Regulation (EC) No. 440/2008 Method C.2 and EPA OPPTS 850.1010. The study was conducted in a static system over a period of 48 hours with a limit concentration of 100 mg/L. 20 daphnids per test concentration and control were tested. The test item concentration was determined at the start and at the end of the test with HPLC-UV and it was in the range of 100 - 105 % of the nominal concentration. Therefore, the biological endpoints were based on the nominal concentration. The validity criteria of the test guideline were fulfilled. There was no immobilisation observed in twenty daphnids exposed to each test item treated and control group. Further no abnormal behaviour or appearance of test animals was noted. The estimated EC50 value (48 hours) of the test item based on the nominal concentration is >100 mg/L.
Reference
Analytical Results
The measured concentration of O-Phospho-L-serine was 105 % of the nominal at the start and 100 % at the end of the test. The measured test item concentration remained within ± 20 % of the nominal during the test period of 48 hours, therefore, the biological results are based on the nominal concentration.
Table 1. Test item measured concentrations
Sampling date |
Nominal |
Mean of the measured concentrations |
Measured concentration in percentage of the nominal |
Relative Standard Deviation % (n=4) |
22 August 2017 |
100 |
105.4 |
105 |
1 |
24 August 2017 |
100 |
100.4 |
100 |
2 |
Table 2. Summary of biological endpoints
Endpoints |
Concentration [mg/L] |
48 h-EC10 |
> 100 |
48 h-EC20 |
> 100 |
48 h-EC50 |
> 100 |
48 h-NOEC |
100 |
48 h-LOEC |
> 100 |
Description of key information
In a short-term toxicity test with Daphnia magna, the test item showed no immobilisation or any other toxic effects up to the limit test concentration. Accordingly, the 48-h EC50 was determined to be >100 mg/L.
Key value for chemical safety assessment
Additional information
In the acute immobilisation test with Daphnia magna (STRAUS), the effects of the test item were determined according to OECD 202, Regulation (EC) No. 440/2008 Method C.2 and EPA OPPTS 850.1010 (reference 6.1.3-1). The study was conducted in a static system over a period of 48 hours with a limit concentration of 100 mg/L. 20 daphnids per test concentration and control were tested. The test item concentration was determined at the start and at the end of the test with HPLC-UV and it was in the range of 100 - 105 % of the nominal concentration. Therefore, the biological endpoints were based on the nominal concentration. The validity criteria of the test guideline were fulfilled. There was no immobilisation observed in twenty daphnids exposed to each test item treated and control group. Further no abnormal behaviour or appearance of test animals was noted. The estimated EC50 value (48 hours) of the test item based on the nominal concentration is >100 mg/L.
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