Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 269-412-8 | CAS number: 68238-92-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- From September 20 to October 19, 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Justification for type of information:
- The read across approach is detailed into the document attached to the IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- Version / remarks:
- 1992
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of activated sludge: ARA Ergolz ll, Füllinsdorf, Switzerland.
- Pretreatment: the sludge was washed by centrifugation, the supernatant liquid phase was decanted and the solid material was resuspended in tap water. This procedure was repeated twice. A homogenized aliquot of the final sludge suspension was weighed, thereafter dried and the ratio of wet to dry weight was calculated.
- Preparation of inoculum for exposure: calculated amounts of wet sludge were suspended in test water to obtain a concentration equivalent to 4 g dry material per liter (± 10 %).
- Method of cultivation: during holding, the sludge was aerated at room temperature until use.
- Initial cell/biomass concentration: defined volumes of the diluted activated sludge were added to test water to obtain a final concentration of 30 mg dry material per liter. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 113 mg/L
- Based on:
- test mat.
- Initial conc.:
- 32.1 - 32.5 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: the test water was prepared according to the testing guidelines.
- Dilution water: the flasks were made up to a volume of 1000 ml with test water.
- Vessels: 2000-ml Erlenmeyer flasks, cleaned with alcoholic hydrochloric acid, rinsed with purified water and dried.
- Test temperature: 22-23 °C.
- pH: prior to test start the pH was 7.4 (measured in all test flasks before the addition of activated sludge (inoculum). At the end of incubation the pH ranged from 7.3 to7.4.
- Aeration of dilution water: each flask was loosely covered with aluminium foil to allow the exchange of air between the flask and the surrounding atmosphere. The test media were continuously stirred by magnetic stirrers.
- Continuous darkness: the test flasks were incubated in a dark room.
SAMPLING
- Sampling frequency: samples were taken on Day 0 (treatment day), 9,7, 10, 14, 21, 27 and 28 of the incubation period for DOC analysis.
- Sampling amount: one sample of about 10 ml was taken from each test flask per sampling date.
- Sampling treatment: samples were filtered through a 0.45 µm filter and analyzed for DOC on the day of sampling.
- Replicate: triplicate per sample.
- Evaporation: water evaporation losses were determined by weighing the flasks and were compensated by adding purified water prior to sampling.
CONTROL AND BLANK SYSTEM
- Inoculum blank: activated sludge, without test item. 1 replicate.
- Reference item: 51-52 mg/l of reference item (i.e. 30.6 - 32.1 mg/l DOC). 2 replicates.
- Abiotic control: 111 mg/l (i.e. 32.1 mg/l DOC) of test item. Poisoned with mercury dichloride at a concentration of 10 mg/l. 1 replicate.
- Toxicity control: 111 mg/l (i.e. 61.0 mg/l DOC) of test item and 51 mg/l of reference item. 1 replicate. - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (DOC removal)
- Value:
- 2 - 8
- Sampling time:
- 28 d
- Details on results:
- ln the test flasks containing the test item and inoculum the mean concentrations of DOC (dissolved organic carbon) varied between 31.6 and 29.8 mg/l over the exposure period of 28 days and were not significantly different from the initial mean DOC concentration of 32.3 mg/l measured on Day 0. Expressed as percentage DOC-removal, mean values in the range from 2 to 8 % were noted. Therefore, test item was found to be not biodegradable under the test conditions.
ABIOTIC CONTROL
ln the abiotic control containing the test item and poisoned medium, the DOC concentrations varied from 31.9 to 29.4 mg/l over the exposure period of 28 days and were not significantly different from the initial DOC concentration of 32.1 mg/l measured at Day 0. Thus, no significant abiotic degradation occurred under the test conditions.
TOXICITY CONTROL
ln the toxicity control containing the test item (corresponding lo 51.2 % of total DOC), the reference item (corresponding lo 48.8 % of total DOC) and inoculum, the initial DOC concentration of 61.0 mg/l measured on Day 0 decreased by 50 % within 14 days of exposure. Thus, according to the test guidelines the test item can be assumed not to be inhibitory to activated sludge because degradation was higher than 35 % within 14 days. - Results with reference substance:
- ln the procedure controls containing the reference item sodium benzoate and inoculum, sodium benzoate was readily biodegraded by 100 % within 7 days of exposure, confirming the suitability of the activated sludge.
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Not readily biodegradable
- Executive summary:
The substance was investigated for the ready biodegradability in a "28-Day DOC Die-Away Test" according to the EU mathod C.4-A and the OECD Guideline 301 A.
In the test flasks containing the test item and inoculum the mean concentrations of dissolved organic carbon (DOC) were not significantly different from the initial mean DOC concentration measured on Day 0. Therefore, test item was found to be not biodegradable under the test conditions.
In the abiotic control containing the test item and poisoned inoculum no degradation was noted atter 28 days of exposure (based on DOC-measurements).
The reference item sodium benzoate was biodegraded by 100 % within 7 days of exposure, confirming the suitability of the activated sludge.
In the toxicity control containing the test item, the reference item sodium benzoate and inoculum, the initial DOC decreased by 50 % within 14 days of exposure. Thus, according to the test guidelines the test item can be assumed to be not inhibitory to activated sludge because degradation was higher than 35 % within 14 days.
Conclusion
Not readily biodegradable
Reference
DOC-removal | % DOC-removal alter x days | ||||||
3 | 7 | 10 | 14 | 21 | 27 | 28 | |
Test item | 6 | 3 | 7 | 5 | 7 | 9 | 9 |
Test item | 1 | 2 | 5 | 5 | 2 | 7 | 4 |
Mean |
3 | 2 | 6 | 5 | 5 | 8 | 7 |
Sodim benzoate | 99 | 100 | 104 | 102 | 100 | 104 | 105 |
Sodim benzoate | 99 | 102 | 104 | 94 | 101 | 103 | 106 |
Mean |
99 | 101 | 104 | 98 | 100 | 104 | 105 |
Toxified solution with test item | 2 | 2 | 8 | 1 | 4 | 4 | 3 |
Test item and sodium benzoate | 46 | 50 | 53 | 50 | 50 | 54 | 53 |
Biodegradation of test item
Vessel | Conc. | mg DOC/l* after x days | |||||||
0 | 3 | 7 | 10 | 14 | 21 | 27 | 28 | ||
Test item | 113 | 32.1 | 30.9 | 33.1 | 31.9 | 32.2 | 30.8 | 32.3 | 31.7 |
Test item | 113 | 32.5 | 32.9 | 34.0 | 32.9 | 32.7 | 33.0 | 33.2 | 33.5 |
Blank | - | 0.0 | 0.7 | 2.0 | 1.9 | 1.7 | 1.1 | 3.0 | 2.4 |
Average of test item flasks, corrected for the blank | - | 32.3 | 31.2 | 31.6 | 30.5 | 30.8 | 30.8 | 29.8 | 30.2 |
* Values given represent mean of three determinations
Biodegradation of sodium benzoate
Vessel | Conc. | mg DOC/l* after x days | |||||||
0 | 3 | 7 | 10 | 14 | 21 | 27 | 28 | ||
Reference item | 51 | 30.6 | 1.1 | 2.0 | 0.7 | 1.0 | 1.1 | 1.8 | ** |
Reference item | 52 | 28.6 | 1.0 | 1.4 | 0.8 | 3.3 | 0.9 | 2.0 | 0.8 |
Blank | - | 0.0 | 0.7 | 2.0 | 1.9 | 1.7 | 1.1 | 3.0 | 2.4 |
Average of ref. item flasks, corrected for the blank | - | 29.6 | 0.4 | -0.3 | -1.2 | 0.5 | -0.1 | -1.1 | -1.5 |
* Values given represent mean of three determinations
**Value not readable
Biodegradation in the toxicity control
Vessel | Conc. | mg DOC/l* after x days | ||||||||
Test item | Sodium benzoate | 0 | 3 | 7 | 10 | 14 | 21 | 27 | 28 | |
Toxified solution with test item | 111 | - | 32.1 | 31.6 | 31.5 | 29.4 | 31.9 | 30.7 | 30.7 | ** |
Test item and sodium benzoate | 112 | 51 | 61.0 | 33.7 | 32.6 | 30.6 | 32.0 | 31.8 | 31.3 | 31.0 |
Test item and sodium benzoate correctd for black | - | - | 61.0 | 33.0 | 30.6 | 28.7 | 30.3 | 30.7 | 28.3 | 28.6 |
Blank | - | - | 0.0 | 0.7 | 2.0 | 1.9 | 1.7 | 1.1 | 3.0 |
2.4 |
* Values given represent mean of three determinations
**Value not readable
Description of key information
Not readily biodegradable
Key value for chemical safety assessment
- Type of water:
- freshwater
Additional information
The substance is a monoazo dye. Reactive Red 147 is not expected to be ready biodegradable because of its chemical structure and the specific function. Commonly, dyes undergo a primary transformation, i.e. discolourization due to the interrupting the conjugation. Nevertheless, the degradation process involves more steps and take more time.
However, there is no experimental information on Reactive Red 147, therefore the available data on the structural analogous Similar Substance 01 has been taken into consideration. The read across approach can be considered as appropriate and suitable to assess the property under investigation (details about the approach are reported into the IUCLID section 13).
The Similarn Substance 01 was investigated for the ready biodegradability in a "28-Day DOC Die-Away Test" according to the EU mathod C.4-A and the OECD Guideline 301 A. In the test flasks containing the test item and inoculum the mean concentrations of dissolved organic carbon (DOC) were not significantly different from the initial mean DOC concentration measured on Day 0. Therefore, test item was found to be not biodegradable under the test conditions.
In the abiotic control containing the test item and poisoned inoculum no degradation was noted atter 28 days of exposure (based on DOC-measurements). In the toxicity control containing the test item, the reference item sodium benzoate and inoculum, the initial DOC decreased by 50 % within 14 days of exposure. Thus, the test item can be assumed to be not inhibitory to activated sludge because degradation was higher than 35 % within 14 days.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.