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EC number: 279-506-0 | CAS number: 80584-92-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1983/12/19 to 1983/12/23
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non GLP study. Guideline from 1981.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Samples for analysis were taken at 0 h and 96h exposure.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Calculated anounts of stock solution to produce the desired test concentrations were given into the water and honogeneously distributed. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: P. Hohler CH-4314 Zeiningen
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 57 mm (49 - 60 mn)
- Weight at study initiation (mean and range, SD): 1.6 g (1.0 - 1.9 g)
- Feeding during test: none
ACCLIMATION
- Acclimation period: 27 days - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 183 mg CaCO3/L
- Test temperature:
- 14 °C
- pH:
- 8 to 8.3
- Dissolved oxygen:
- 80 to 101 %
- Nominal and measured concentrations:
- 100, 180, 320, 580, 1000 mg/L (nominal)
99.7, 176.7, 310.1, 541.0, 972.5 mg/L (measured) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria of 20 liters filled with 15 liters (36/22/25 cm)
- Aeration: Gentle aeration after 24 h exposure
- No. of organisms per vessel: 5 fishes per aquarium
- No. of vessels per concentration (replicates): two replicates
- No. of vessels per control (replicates): two replicates
- Biomass loading rate: 0.5 g/L
TEST MEDIUM / WATER PARAMETERS
- in accordance to the guideline
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours daily
- Light intensity: Fluorescenet light
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
mortality, visible abnormalities were measured after 24, 48, 72 and 96 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: approx. 1.8 - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: no behaviour abnormalities were observed
- Mortality of control: no mortality
- Other adverse effects control: no effects observed - Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: APHA method (1980)
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on sampling:
- - concentrations were analysed by gas-liquid chromatography (GLC)
- Vehicle:
- no
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- Test species: Fathead minnow (Pimephales promelas)
- age (d): 30
- mean body length (mm): 18.1 ± 0.788
- mean weight (g): 0.083 ± 0.0083
- loading (g/l): 0.1153 - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 25.7 ± 0.38 °C
- pH:
- 7.8 ± 0.07
- Dissolved oxygen:
- 7.3 mg/l
- Nominal and measured concentrations:
- Nominal test concentrations (g/l): 0, 7.30, 11.2, 17.3, 26.6, and 40.9
test substance recovery by GLC: 107.2 ± 3.8% (n = 4) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 20 fish/concentration
- tank volume (l): 1.0 (additions 14.4 v/d)
- pH: the pH of the stock solution (90.4 mg/l) was adjusted to that of lake water using HCl. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 11 800 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: pH was adjusted to that of lake water
- Reported statistics and error estimates:
- The estimated LC/EC50 values with corresponding 95% confidence intervals were calculated using the corrected average of the analyzed tank concentrations and the Trimmed Spearman-Kaerber method (Hamilton et al., 1977).
- Sublethal observations / clinical signs:
Effect values (related to measured concentrations): 96-h EC50 (g/l): 11.8 (10.6-13.0)
Mortalities
Nominal conc (g/l)
Mean measured (corr.)
No. of fish at start
Dead fish after
24h
48h
72h
96h
Control
< 0.02
20
0
0
0
0
7.30
6.14
20
0
0
0
0
11.2
9.86
20
1
3
4
4
17.3
15.4
20
15
17
18
18
26.6
24.4
20
20
20
20
20
40.9
38.1
20
20
20
20
20
Physiological/behavioral effects
Affected fish showed loss of schooling behaviour, hyperactivity, dark coloration, increased respiration and loss of equilibrium prior to death.- Validity criteria fulfilled:
- yes
Referenceopen allclose all
Description of key information
The compound consists of a triazine part and an amine part. Under environmental conditions it is present in its dissociated form. Therefore, separate assessments were conducted for the triazine and the amine part, respectively.
CAS 80584 -91 -4: There is a high probability that the compound is not acutely harmful to fish.
CAS 102 -71 -6: There is a high probability that the compound is not acutely harmful to fish.
Key value for chemical safety assessment
Additional information
The compound consists of a triazine part and an amine part. Under environmental conditions it is present in its dissociated form. Therefore, separate assessments were conducted for the triazine and the amine part, respectively.
CAS 80584-91-4
The acute toxicity to fish was assessed in a key and supporting study. In the key study rainbow trout and in the supporting study zebra fish, were exposed under static conditions to nominal concentrations of 100, 180, 320, 580 and 1000 mg/L. In both studies the LC50 was determined to be > 1000 mg/L.
CAS 102-71-6
To determine the toxicity of 2,2’,2’’-nitrilotriethanol to fish, a non-GLP study similar to APHA method using Pimephales promelas and a flow-through procedure was performed by Geiger et al. (1990). The calculated LC50 value after 96 hours in this study was 11800 mg/l related to the nominal concentrations (confirmed by concentration control analytics). Hence, the chemical is considered to be acutely not harmful for fish. Other data which are available for 2,2’,2’’-nitrilotriethanol are disregarded and shout not be used for risk assessment purposes mainly due to the insufficient exposure duration.
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