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EC number: 229-962-1 | CAS number: 6864-37-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The substance is acutely toxic to aquatic algae.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 7.9 mg/L
- EC10 or NOEC for freshwater algae:
- 4.1 mg/L
Additional information
In order to determine the effects of 4,4'-methylenebis(2-methylcyclohexanamine) (CAS 6864-37-5, DMDC) on aquatic algae, there are data from two studies available:
1.) In a non-GLP study according to OECD Guideline 201, conducted with Desmodesmus subspicatus (BASF AG, 1989), the 72-h ErC50 was determined to be >5.0 mg/L (nominal; analytically not verified; 72-h ErC10 = 1.25 mg/L).
2.) In an additional GLP-study (NITE Japan, 2002), performed according to OECD 201 (Alga, Growth Inhibition Test) with Pseudokirchneriella subcapitata, the test concentrations were analytically verified. The results were recalculated with Toxrat v2.10 (BASF SE 2014); the measured geomean concentrations in the relevant concentration range were within ±20% of the nominal values. Therefore, the results are based on the measured geomean concentrations. As analytical monitoring was only performed for this study by NITE (2002), these values are selected as key data. The 72-h ErC50 was estimated to be 7.9 mg/L. The 72-h ErC10 was determined to be 4.1 mg/L and the 72-h NOEC was 0.13 mg/L. All validity criteria were fulfilled in this study.
According to the Guidance on information and safety assessment Chapter R.10: Characterisation of dose [concentration]-response for environment “an EC10 for a long-term test which is obtained using an appropriate statistical method (usually regression analysis) will be used preferentially. […] There has been a recommendation within OECD in 1996 to phase out the use of NOEC, in particular as it can correspond to large and potentially biologically important magnitudes of effect. The advantage of regression method for the estimation of ECx is that information from the whole concentration-effect relationship is taken into account and that confidence intervals can be calculated. These methods result in an ECx, where x is a low effect percentile (e.g. 5-20%). It makes results from different experiments more comparable than NOECS”.
Therefore, the ErC10 (4.1 mg/L; NITE, 2002) instead of the NOEC has been used for the assessment.
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