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EC number: 419-210-2 | CAS number: 178452-71-6 OLIVE-GREEN JB 1170
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Version / remarks:
- Adopted February 24, 1987.
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.3 (Acute Toxicity (Dermal))
- Version / remarks:
- July 31, 1992.
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- fixed dose procedure
- Limit test:
- yes
Test material
- Reference substance name:
- Pentasodium bis{7-[4-(1-butyl-5-cyano-1,2-dihydro-2-hydroxy-4-methyl-6-oxo-3-pyridylazo)phenylsulfonylamino]-5'-nitro-3,3'-disulfonatonaphthalene-2-azobenzene-1,2'-diolato} chromate (III)
- EC Number:
- 419-210-2
- EC Name:
- Pentasodium bis{7-[4-(1-butyl-5-cyano-1,2-dihydro-2-hydroxy-4-methyl-6-oxo-3-pyridylazo)phenylsulfonylamino]-5'-nitro-3,3'-disulfonatonaphthalene-2-azobenzene-1,2'-diolato} chromate (III)
- Cas Number:
- 178452-71-6
- Molecular formula:
- C66H48CrN16Na5O28S6
- IUPAC Name:
- chromium(3+) pentasodium bis(7-{4-[2-(1-butyl-5-cyano-2-hydroxy-4-methyl-6-oxo-1,6-dihydropyridin-3-yl)diazen-1-yl]benzenesulfonamido}-2-[2-(5-nitro-2-oxido-3-sulfonatophenyl)diazen-1-yl]-3-sulfonatonaphthalen-1-olate)
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Remarks:
- Hanlbm
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
-Source: BRL, Biological Research Laboratories Ltd. Wölferstrasse 4, 4414 Füllinsdorf / Switzerland
-Age at study initiation:
males: 8 weeks
females: 11 weeks
-Weight at study initiation:
males: 223.7 - 247.1 g
females: 191.6 - 201.9 g
-Housing: Five in Makrolon type-4 cages with autoclaved standard softwood bedding ("Lignocel", Schill AG, CH-4132 Muttenz). During treatment and observation individually in Makrolon type-3 cages with autoclaved standard softwood bedding.
-Diet: pelleted standard Kliba 343, Batch no. 82/94 rat maintenance diet ("Kliba", Klingentalmuehle AG, CH-4303 Kaiseraugst), available ad libitum.
-Water: community tap water from Füllinsdorf, available ad libitum.
-Acclimation period: one week
ENVIRONMENTAL CONDITIONS
-Temperature: 19 - 20.5 °C
-Humidity: 46 - 62 %
-Air changes: 10-15 air changes per hour
-Photoperiod: 12 hours artificial fluorescent light (approx. 100 Lux) / 12 hours dark (light period between 6.00 a.m. to 6.00 p.m.)
Administration / exposure
- Type of coverage:
- semiocclusive
- Vehicle:
- water
- Details on dermal exposure:
- TEST SITE
-Area of exposure: ~ 10% of the total body surface
-Type of wrap if used: covered with a semi-occlusive dressing. The dressing was wrapped around the abdomen and fixed with an elastic adhesive bandage.
REMOVAL OF TEST SUBSTANCE
-Washing: with lukewarm tap water and dried with disposable paper towels.
-Time after start of exposure: 24 hours after the application
TEST MATERIAL
-Application volume/kg body weight: 4 ml/kg - Duration of exposure:
- 24 hours
- Doses:
- 2000 mg/kg b.w.
- No. of animals per sex per dose:
- 5 per sex per dose
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations:
Mortality / Viability: four times during test day 1 and daily during days 2-15.
Bodyweights: On test days 1, 8 and 15.
Clinical Signs: Clinical Signs Each animal was examined for changes in behaviour and appearance (with special emphasis on the application area, except for the time when the semi-occlusive dressing was in place) four times during day 1, and once daily during days 2-15. All abnormalities were recorded.
- Necropsy of survivors performed: yes.
Necropsies were performed by experienced prosectors. At the end of the observation period all animals were anesthetized by intraperitoneal injection of Narcoren at a dose of at least 2,0 ml/kg body weight (equivalent to at least 320 mg/kg sodium pentobarbitone/kg body weight) and sacrificed by exsanguination. The animals were examined macroscopically and all abnormalities recorded. - Statistics:
- The LOGIT-Model could not be used as no deaths occurred.
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- There were no deaths as a result of treatment with the test substance.
- Clinical signs:
- other: Yellow discoloration was observed at the application site after the removal of the dressing until study termination (day 15). In one male animal (no.3) this observation was noted until test day 12.
- Gross pathology:
- No organ abnormalities were observed at necropsy.
Applicant's summary and conclusion
- Interpretation of results:
- other: Not classified according to the CLP Regulation (EC 1272/2008)
- Conclusions:
- LD50 (rat) was found to be greater than 2000 mg/kg body weight.
- Executive summary:
The substance was tested for acute toxicity by dermal route according to the OECD Guidelines 402 (1987) and the method B.3 of Directive 92/69/EEC. The test substance was administered to a group of 5 male and 5 female rats by dermal application at a single dose of 2000 mg test article/kg body weight. No deaths occurred during the study period. Yellow discoloration was observed at the application site after the removal of the dressing until study termination (14 days after the treatment). However in one male animal this observation was noted until test day 12. There were no test substance-related effects on the body weight of the animals during the observation period. The minimal to slight loss of body weight in one female animals during the first observation week was considered to be a consequence of the semi-occlusive dressing. Commonly female animals prove to be more sensitive in relation to the effects on body weight caused by semi-occlusive dressing than males. The macroscopic examination at study termination revealed no organ abnormalities.
The LD50 (rat, dermal) was found to be greater than 2000 mg/kg body weight.
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