Registration Dossier
Registration Dossier
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EC number: 247-724-5 | CAS number: 26472-00-4
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well conducted, GLP compliant, study conducted in accordance with internationally recognised test methods
Data source
Reference
- Reference Type:
- other: USEPA High Production Volume Information System (HPVIS)
- Title:
- Unnamed
- Year:
- 2 002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- not specified
- GLP compliance:
- yes
Test material
- Reference substance name:
- Methylcyclopentadiene
- EC Number:
- 247-724-5
- EC Name:
- Methylcyclopentadiene
- Cas Number:
- 26472-00-4
- Molecular formula:
- not applicable, UVCB substance
- IUPAC Name:
- 3a,4,7,7a-tetrahydro-1H-4,7-methanoindene
- Test material form:
- liquid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Clinton Sanitary Wastewater Treatment Plant, Annandale, NJ, USA
- Concentration of sludge: 3.74 g/L total suspended solids
- Initial cell/biomass concentration: 10E5 CFU / mL
: - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 49.77 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST SYSTEM
- Culturing apparatus: Coordinated Environmental Services automated respirometer
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: Aeration of test medium prior to test start
- Measuring equipment: Automated respirometer
- Test performed in open system: No
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, triplicate
- Abiotic sterile control: No data
- Toxicity control: No data
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 48.49 mg/L
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- >60% degradation within 14 days
98% degradation within 28 days
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test substance is not readily biodegradable
- Executive summary:
The test substance is not readily biodegradable.
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