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EC number: 255-473-8 | CAS number: 41642-51-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February 6, 1979 to March 26, 1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Inhalation toxicity was tested according to the method of Sachsse et al. (1973, 1976).
- Deviations:
- no
- Principles of method if other than guideline:
- K. Sachsse, L. Ullmann, G. Voss and R. Hess: Measurement of inhalation toxicity of aerosols in small laboratory animals. In: Proceedings of the Europ. Soc. for the Study of Drug Toxicity. Vol XV, pp. 239-251, Zurich, June 1973.
K. Sachsse, L. Ullmann, K. Zbinden: Toxikologische Prüfungen von Aerosolen im Tierexperiment: Aus "Chemische Rundschau" 29 (1976), Nr. 38 Seite 1-4. - GLP compliance:
- no
- Remarks:
- Pre-dates GLP
- Test type:
- traditional method
- Limit test:
- no
Test material
- Reference substance name:
- N-[2-[(2,6-dicyano-4-nitrophenyl)azo]-5-(diethylamino)phenyl]acetamide
- EC Number:
- 255-473-8
- EC Name:
- N-[2-[(2,6-dicyano-4-nitrophenyl)azo]-5-(diethylamino)phenyl]acetamide
- Cas Number:
- 41642-51-7
- Molecular formula:
- C20H19N7O3
- IUPAC Name:
- N-[2-[(2,6-dicyano-4-nitrophenyl)azo]-5-(diethylamino)phenyl]acetamide
- Test material form:
- solid: particulate/powder
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Tif: RAIf
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- The test was performed with 30 male and 30 female young adult rats of the Tif: RAIf (SPF) strain, raised on the premises. An additional group of 20 (10 males/10 females) rats served as a control.
Before and after the inhalation the animals were kept at a room temperature of 22 + 2° C, at a relative humidity of 55 + 10 % and on a 10 hours light cycle day. They received ad libitum rat food - NAFAG, Gossau SG - and water. Prior to treatment they were adapted to our laboratories for a minimum of 4 days. Bodyweights were recorded immediately prior to exposure (control weights) and at day 7 and 14 (Table 2). The males and females were segregated and kept in Macrolon cages, type 4, (10 animals to a cage), individually marked with picric acid.
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- nose only
- Vehicle:
- air
- Details on inhalation exposure:
- Preparation of aerosol
The aerosol was generated by injecting the solid test material with the help of a Grafix Exaktomat Injector (Cerutti AG, Bern, Switzerland) into an air stream which was discharged into the exposure chamber at a rate of 20 l/min. The control animals were exposed to filtered air under the same conditions as described above.
The concentration and the particle size distribution of the aerosol in the vicinity of the animals were monitored at regular intervals throughout the aerosol exposure. The concentration was determined 5 times gravimetrically by sampling the test atmosphere through a selectron filter of 50 mm diameter and with a pore size of 0.2 μm (Schleicher and Schuell, Feldbach, Switzerland) at an air flow rate of 10 1/min. The size distribution of the particles was measured twice with a 4 stage Cascade Impactor with selectron filters of 25 mm diameter and with a pore size of 0.2 μm (Schleicher and Schuell) at an air flow rate of 17.5 l/min. - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- 107 ± 6 mg/m3, 194 ± 8 mg/m3 and 317 ± 22 mg/m3
- No. of animals per sex per dose:
- 10 male/10 female per dose
- Control animals:
- yes
- Details on study design:
- Testing procedures
For inhalation the rats were kept in separate PVC tubes positioned radially around the exposure chamber such that snout and nostrils of the animals only were exposed to the aerosol.
During the exposure period the following parameters were controlled once at half time of the study inside the inhalation cylinder: temperature (with a Therm 2104 contact thermometer, Ahlborn Messund Regeltechnik, 815 Holzkirchen, Germany), relative humidity (with a VASALA Humidity Indicator HMI 11, Kelag AG, 8057 Zurich, Switzerland) and oxygen content (with a DRAEGER E 15 stationary control system, Draegerwerk AG, Lübeck, Germany).
After a 4 hour inhalation the rats were returned to their cages. Physical condition and incidence of death were monitored throughout an observation period of 14 days. - Statistics:
- Not specified in the study report.
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 300 mg/m³ air
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- No mortality
- Clinical signs:
- other: The animals exposed to the test material recovered within 3 to 4 days. They were submitted to a necropsy at the end of the observation period. The control rats failed to show any symptoms, during the exposure and observation period.
- Body weight:
- Reported in table form - see Any other information
- Gross pathology:
- Partially congested organs were observed.
- Other findings:
- Particle size distribution analysis of the chamber airborne particles showed that 20 to 60 % were smaller than 7 μm in diameter.
Any other information on results incl. tables
Temperature, relative humidity and oxygen control
The following values were obtained during the exposure period.
|
Control group 0 mg/m3 |
107 ± 6 mg/m3 |
194 ± 8 mg/m3 |
317 ± 22 mg/m3 |
Temperature °C |
23 |
24 |
24 |
24 |
Relative Humidity % |
58 |
40 |
42 |
55 |
Oxygen content Vol. % |
20 |
20 |
20 |
20 |
RATE OF DEATHS
Dose mg/m3 |
Sex |
Total Number animals in group |
Total Number animals dead |
Death rate percentage |
|
||||
0 Control |
Male |
10 |
0 |
0 |
107 ± 6 |
10 |
0 |
0 |
|
194 ± 8 |
10 |
0 |
0 |
|
317 ± 22 |
10 |
0 |
0 |
|
|
||||
0 Control |
Female |
10 |
0 |
0 |
107 ± 6 |
10 |
0 |
0 |
|
194 ± 8 |
10 |
0 |
0 |
|
317 ± 22 |
10 |
0 |
0 |
BODYWEIGHT CHANGE
|
Concentration mg/m3 |
||||
0 Control |
107 ± 6 |
194 ± 8 |
317 ± 22 |
||
Day 1 Male |
MEAN BODYWEIGHT (g) |
237 |
230 |
238 |
187 |
Day 1 Female |
205 |
194 |
191 |
181 |
|
Day 7 Male |
260 |
255 |
247 |
219 |
|
Day 7 Female |
212 |
204 |
191 |
189 |
|
Day 14 Male |
290 |
303 |
292 |
273 |
|
Day 14 Female |
221 |
224 |
210 |
209 |
Signs and symptoms
Concentration (mg/m3): 107 ± 6 |
||||||||||||||||||
Signs and symptoms |
Exposure Hours |
Days |
||||||||||||||||
1 |
2 |
4 |
6 |
24 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
|
Sedation |
|
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Dyspnoea |
|
+ |
+ |
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Dacryorrhoea |
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Chromodacryorrhoea |
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Rinorrhoera |
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Epistaxis |
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Exophthalmos |
|
+ |
+ |
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+ |
+ |
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Salivation |
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Ruffled fur |
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+ |
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+ |
+ |
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Pallor |
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Cyanosis |
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Diarrhoea |
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Body position (ventral) |
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Body position (lateral) |
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Body position (curved) |
|
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+ |
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Ataxia |
|
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Trismus |
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Tremor |
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Tonic clonic muscle spasms |
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Convulsions |
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Key: + = slight, ++ = moderate, +++ = severe
Concentration (mg/m3): 194 ± 8 |
||||||||||||||||||
Signs and symptoms |
Exposure Hours |
Days |
||||||||||||||||
1 |
2 |
4 |
6 |
24 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
|
Sedation |
|
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|
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Dyspnoea |
|
+ |
+ |
|
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Dacryorrhoea |
|
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Chromodacryorrhoea |
|
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Rinorrhoera |
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Epistaxis |
|
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Exophthalmos |
|
+ |
+ |
|
+ |
+ |
|
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|
|
|
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|
|
|
|
|
|
Salivation |
|
|
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Ruffled fur |
|
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+ |
|
+ |
+ |
|
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|
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Pallor |
|
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|
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Cyanosis |
|
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Diarrhoea |
|
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Body position (ventral) |
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Body position (lateral) |
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Body position (curved) |
|
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+ |
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+ |
+ |
|
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Ataxia |
|
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Trismus |
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Tremor |
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Tonic clonic muscle spasms |
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Convulsions |
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Key: + = slight, ++ = moderate, +++ = severe
Concentration (mg/m3): 317 ± 22 |
||||||||||||||||||
Signs and symptoms |
Exposure Hours |
Days |
||||||||||||||||
1 |
2 |
4 |
6 |
24 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
|
Sedation |
|
|
|
|
|
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Dyspnoea |
|
+ |
+ |
|
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|
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Dacryorrhoea |
|
|
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Chromodacryorrhoea |
|
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|
|
|
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|
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|
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|
Rinorrhoera |
|
|
|
|
|
|
|
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|
|
|
|
|
|
|
|
Epistaxis |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Exophthalmos |
|
+ |
+ |
|
+ |
+ |
|
|
|
|
|
|
|
|
|
|
|
|
Salivation |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Ruffled fur |
|
|
+ |
|
+ |
+ |
|
|
|
|
|
|
|
|
|
|
|
|
Pallor |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Cyanosis |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Diarrhoea |
|
|
|
|
|
|
|
|
|
|
|
|
|
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|
Body position (ventral) |
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Body position (lateral) |
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Body position (curved) |
|
|
+ |
|
+ |
+ |
|
|
|
|
|
|
|
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|
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|
|
Ataxia |
|
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|
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|
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Trismus |
|
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Tremor |
|
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|
Tonic clonic muscle spasms |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
|
|
|
Convulsions |
|
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|
|
|
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Key: + = slight, ++ = moderate, +++ = severe
Applicant's summary and conclusion
- Interpretation of results:
- other: LC50 is greater than the highest dose tested in this study.
- Conclusions:
- The LC50 of a 4 hour aerosol exposure for rats of both sexes is greater than 300 mg/cubic-meter air, when evaluated for a 14 day post treatment observation period.
- Executive summary:
Inhalation toxicity was tested according to the method of Sachsse et al. (1973, 1976).
For inhalation the rats were kept in separate PVC tubes positioned radially around the exposure chamber such that snout and nostrils of the animals only were exposed to the aerosol.
Results
Particle size distribution analysis of the chamber airborne particles showed that 20 to 60 % were smaller than 7 μm in diameter.
The animals exposed to the test material recovered within 3 to 4 days. They were submitted to a necropsy at the end of the observation period.
The control rats failed to show any symptoms, during the exposure and observation period.
The LC50 of a 4 hour aerosol exposure for rats of both sexes is greater than 300 mg/cubic-meter air, when evaluated for a 14 day post treatment observation period.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.