Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 609-330-5 | CAS number: 370865-89-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Remarks:
- Scientific research
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Name of test material (as cited in publication): 1-ethyl-3-methylimidazolium dicyanimide (IM12 [N(CN)2])
- Source: Merck KGaA (Darmstadt, Germany)
- Purity: ≥ 97% - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Direct application in the medium.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The assessment of the test was carried out using the commercially available DaphToxkit (MicroBioTest Incorporation, Gent, Belgium).
- Age of test organisms at study initiation: less than 24 hours old
- Feeding: the organisms were pre-fed - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20 °C
- Nominal and measured concentrations:
- 5 different concentration and a control were investigated, concentrations are not further specified.
- Details on test conditions:
- TEST SYSTEM
- Fill volume: 10 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration: 5
- No. of vessels per control: 5
- Other: The experiment was conducted twice
OTHER TEST CONDITIONS
- Illumination: tests were performed in the dark
EFFECT PARAMETERS MEASURED: Immobility or mortality was recorded after 24 and 48 h. - Reference substance (positive control):
- yes
- Remarks:
- The sensitivity of the organisms to K2Cr2O7 was checked routinely once a new batch of organisms was obtained.
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 150 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: corresponds to 860 µM (97.5% C.I.: 820 - 900 µM)
- Results with reference substance (positive control):
- No data presented.
- Reported statistics and error estimates:
- Dose-response curve parameters and plots were done using the drfit package (version 0.05-92) for the R language and environment for statistical computing.
- Validity criteria fulfilled:
- not specified
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
The nominal concentrations were prepared by dilution of a stock solution of the test substance in aerated Daphnia medium. 100 ml of the test solutions were filled in the first of the two replicate test vessels. The solution was aerated by stirring for about 0.5 h. O2 concentration and pH was measured. The pH was adjusted, where necessary. Subsequently, half of the solution (50 ml) was filled in the 2nd replicate test vessel. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna STRAUS
- Age: less than 24 h
- Feed: Suspension of Desmodesmus subspicatus in Elendt M4 medium with an optical density OD680 of about 15 units; 400 μl per Daphnia per day, except on weekends - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2.52 mmol/L
- Test temperature:
- 22 °C
- pH:
- 7.1 - 7.4
- Dissolved oxygen:
- 6.0 - 6.5 mg/L
- Nominal and measured concentrations:
- - Nominal concentrations: 0 (control), 25.0, 50.0, 100, 200 and 400 mg/L
- Details on test conditions:
- TEST SYSTEM
- Number of Daphnia: 40 individuals per test concentration, 20 per vessel
- Test vessel: 100 ml flasks, all-glass, with 50 ml of test medium
- Replicates: 2 replicates with test animals (20 animals per replicate); 3 replicates as blank controls
TEST MEDIUM
- Medium: Contious aerated Elendt M4 medium; prepared with deionised water (conductivity <1.5 µS/cm)
OTHER TEST CONDITIONS
- Light: 16 h photoperiod a day, supplied by oberhead white fluorescent tubes
EFFECT PARAMETERS MEASURED: Observations of immobile Daphnia were made after 24 and 48 h of exposure. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (Acute reference test with potassium dichromate conducted twice a year)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 190 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 53.2-346
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 84.8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 0.544-149
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 106 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- other: cation
- Basis for effect:
- mobility
- Remarks on result:
- other: 21.4-199
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 48.9 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- other: cation
- Basis for effect:
- mobility
- Remarks on result:
- other: 0.049-85.1
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Name of test material (as cited in publication): 1-Ethyl-3-methylimidazolium dicyanamide [EMIM] [DCA]
- Source: BASF et CYTEC
- Purity: ≥ 98% - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The required amount of the test substance was introduced directly into dilution water, which was then magnetically stirred for 2 minutes.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISMS
- Common name: Water flea
- Clone: Straus (clone A)
- Age: Neonate organisms of less than 24 hours old, obtained from parthogenetic females of 2 to 5 weeks old were selected for the test.
ACCLIMATION
- Acclimation conditions: The test organisms were kept in a 5 L glass beaker, filled with 2 L of Elendt M4 medium with a temperature of 18 - 22 °C and 16-h light:8-h dark photoperiod. The medium was renewed once every week.
- Feeding: In the stock culture the organisms were fed with unicellular green algae (Chlorella vulgaris/Desmodesmus subspicatus).
- Loading rate: In the stock culture, a loading rate of 20 animals per litre was maintained. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 18 - 22 °C
- Nominal and measured concentrations:
- The nominal concentrations of the test substance ranged from 47.7 to 500 mg/L. The concentrations were based on previous preliminary range-finding tests. No further specification given.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass test tubes
- Fill volume: 10 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4
OTHER TEST CONDITIONS
Tests were performed in the dark
EFFECT PARAMETERS MEASURED: Immobilisation was recorded after 24 and 48 hours exposure. - Reference substance (positive control):
- yes
- Remarks:
- The sensitivity to K2Cr2O7 was investigated to validate the selected batches of test organisms.
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 247.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% C.I.: 248.5 - 298.4 mg/L
- Remarks:
- test 1
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 268.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% C.I.: 231.7 - 255.1 mg/L
- Remarks:
- test 2
- Results with reference substance (positive control):
- No data presented.
- Reported statistics and error estimates:
- EC50 values were calculated from the concentration-response curves using a logistic Hill model. The 95% confidence intervals were estimated using a “bootstrap” simulation method. Calculations were done with REGTOX software v.7.0.5.
- Validity criteria fulfilled:
- not specified
Referenceopen allclose all
Description of key information
The 48-h EC50 value is 190 mg/L in aquatic invertebrates (Daphnia magna).
Key value for chemical safety assessment
Additional information
The acute toxicity to aquatic invertebrates was determined according to OECD TG 202 and in compliance with GLP criteria (BMG, 2013). In this study daphnids (D. magna, 40 per concentration) were exposed to nominal concentrations of 0 (control), 25, 50, 100, 200 and 400 mg/L under static conditions. Nominal test concentrations were analytically confirmed. The effect concentrations are expressed as the geometric mean of the measured concentrations. Mobility was scored as parameter for toxicity. The 48-h EC50 value of the test substance was determined at 190 mg/L.
Supporting data is available from two non-GLP publications that were performed according to, or similar to, OECD TG 202. Steudte et al (2012) exposed a total of 25 daphnids (D. magna) per concentration, to several concentrations (and a control) of the test substance for 48 hours under static freshwater conditions in the dark. Bado-Nilles et al (2015) exposed daphnids (D. magna, 20 per concentration) to nominal concentrations that ranged from 47.7 to 500 mg/L, for 48 hours under static conditions in the dark. In both studies, no test concentrations were analytically verified. Immobilisation was recorded after 24 and 48 hours exposure. In both studies, the experiments were conducted twice. Steudte and his group determined the 48-h EC50 to be 860 µM, corresponding to 150 mg/L. Bado-Nilles and colleagues found 48-h EC50 values of 248.5 and 298.4 mg/L, respectively.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.