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EC number: 219-546-8 | CAS number: 2459-09-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- data is from peer reviewed journals
Data source
Reference
- Reference Type:
- publication
- Title:
- An Animal Model for Non-immunologic Contact Urticaria
- Author:
- ARTO LAHTI AND HOWARD I. MAIBACH
- Year:
- 1 984
- Bibliographic source:
- TOXICOLOGY AND APPLIED PHARMACOLOGY 76, 2, 19-224, (1984)
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- A study was performed to assess irritation potential of methyl nicotinate on guinea pigs
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Methyl nicotinate
- EC Number:
- 202-261-8
- EC Name:
- Methyl nicotinate
- Cas Number:
- 93-60-7
- Molecular formula:
- C7H7NO2
- IUPAC Name:
- 3-Pyridinecarboxylic acid methyl ester
- Test material form:
- solid
- Details on test material:
- - Name of test material (as cited in study report): Methyl nicotinate
- Molecular formula: C7H7NO2
- Molecular weight: 137.137 g/mol
- Smiles notation: c1(C(OC)=O)cccnc1
- InChI: 1S/C7H7NO2/c1-10-7(9)6-3-2-4-8-5-6/h2-5H,1H3
- Substance type: Organic
- Physical state: Solid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Methyl nicotinate
- Molecular formula: C7H7NO2
- Molecular weight: 137.137 g/mol
- Smiles notation: c1(C(OC)=O)cccnc1
- InChI: 1S/C7H7NO2/c1-10-7(9)6-3-2-4-8-5-6/h2-5H,1H3
- Substance type: Organic
- Physical state: Solid
- obtained from Sigma Aldrich (St. Louis, Mo., USA)
Test animals
- Species:
- guinea pig
- Strain:
- Hartley
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Sex: female
- Source: Charles River Breeding Laboratories, Wilmington, Mass, USA
- Age at study initiation: no data
- Weight at study initiation: weighing 350 to 500 g
- Fasting period before study: no data
- Housing: five per cage
- Diet (e.g. ad libitum): Guinea Pig Diet, Wayne Laboratory
Animal Diets, Chicago; ad libitum
- Water (e.g. ad libitum): water; ad libitum
- Acclimation period: no data
ENVIRONMENTAL CONDITIONS
- Temperature (°C): temperature-controlled (23±1 “C) rooms.
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): artificially illuminated ( 12 hr/day)
Test system
- Type of coverage:
- open
- Preparation of test site:
- shaved
- Vehicle:
- other: Absolute ethyl alcohol
- Controls:
- yes, concurrent vehicle
- Amount / concentration applied:
- 0.2% methyl nicotinate in absolute ethyl alcohol, 20µl to each test site
- Duration of treatment / exposure:
- 15 minutes
- Observation period:
- The thickness of the ear was measured 5 min after the application, and then every 10 min during the first hour, every 15 min during the second, and every 30 min during the third hour.
- Number of animals:
- Five guinea pigs, each animal with five test sites
- Details on study design:
- TEST SITE
- Area of exposure: The following sites were selected:
upper back, lower back, upper abdomen, lower abdomen, and flank.
- % coverage: The test sites were marked with a plastic ring stamp, 17 mm in diameter.
- Type of wrap if used: no data
REMOVAL OF TEST SUBSTANCE
- Washing (if done):yes
- Time after start of exposure: after 15 minutes of exposure, the test chemical was washed away with a soft sponge and lukewarm water.
Results and discussion
In vitro
- Other effects / acceptance of results:
- no data available
In vivo
Results
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- other: 5 minutes after application
- Reversibility:
- not specified
- Remarks on result:
- positive indication of irritation
- Irritant / corrosive response data:
- The reactivity in different test sites varied greatly, the upper back being the most sensitive part of the guinea pig body. The strength of the reactions varied from slight to intensive erythema without edema.
Any other information on results incl. tables
Table 1: ERYTHEMA REACTIONS TO METHYL NICOTINATEaON DIFFERENT SITES OF GUINEA PIGSb BODY SKIN
Test substance |
Upper back |
Lower back |
Upper abdomen |
Lower abdomen |
Flank |
Methyl nicotinate |
4c |
4 |
1 |
0 |
0 |
a- 0.2% in absolute ethyl alcohol, 20µl to each test site
b- Five guinea pigs, each animal with 5 test sites
c- Number of animals with erythema reactions
Applicant's summary and conclusion
- Interpretation of results:
- Category 2 (irritant) based on GHS criteria
- Conclusions:
- Erythema reactions from methyl nicotinate appeared within 5 minutes of application.The reactivity in different test sites varied greatly, the upper back being the most sensitive part of the guinea pig body. The strength of the reactions varied from slight to intensive erythema without edema.
Hence, methyl nicotinate can be considered to be irritating to guinea pig skin. - Executive summary:
A study was performed to assess irritation potential of methyl nicotinate on guinea pigs. Six groups of 5 Hartley guinea pigs were used.Hair on the back, abdomen, and flank was shaved with an electric razor; remaining short hair was removed with a depilatory cream. These procedures did not cause any visible changes in guinea pig skin. The test sites were marked with a plastic ring stamp, 17 mm in diameter. The following sites were selected: upper back, lower back, upper abdomen, lower abdomen, and flank. 20µl of 0.2% methyl nicotinate in absolute ethyl alcohol was applied to the shaved test sites for 15 minutes. After 15 minutes of exposure, the test chemical was washed away with a soft sponge and lukewarm water. The thickness of the ear was measured 5 min after the application, and then every 10 min during the first hour, every 15 min during the second, and every 30 min during the third hour.
Erythema reactions from methyl nicotinate appeared within 5 minutes of application. The reactivity in different test sites varied greatly, the upper back being the most sensitive part of the guinea pig body. The strength of the reactions varied from slight to intensive erythema without edema.
Hence, methyl nicotinate can be considered to be irritating to guinea pig skin.
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