Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-636-9 | CAS number: 108-99-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991-1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This is a GLP OECD guideline study undertaken by the Institute of Hygiene and Epidemiology, Division of Toxicology, in Brussels, BE, in preparation for the OECD High Production Volume chemicals programme.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3-methylpyridine
- EC Number:
- 203-636-9
- EC Name:
- 3-methylpyridine
- Cas Number:
- 108-99-6
- Molecular formula:
- C6H7N
- IUPAC Name:
- 3-methylpyridine
- Details on test material:
- 98% purity
Constituent 1
Method
- Target gene:
- His-
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- Standard cell lines according to OECD 471. For TA 1535: his G46 base substitution, rfa and -uvrB; for TA 100: his G46 base substitution, rfa, -uvrB and R factor; for TA 1537: his C3076 frameshift mutation, rfa and -uvrB; for TA 98: his C3052 frameshift mutation, rfa, -uvrB and R factor.
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 fraction from livers of CD rats exposed to Arochlor 1254
- Test concentrations with justification for top dose:
- 50 (0.51 microM), 160 (1.63 microM), 500 (5.10 microM), 1600 (16.30 microM), and 5000 nl/plate (51.00 microM)
- Vehicle / solvent:
- water
Controlsopen allclose all
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- for strain TA 1535 without S-9
Migrated to IUCLID6: 0.5 microgram/plate
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- Positive controls:
- yes
- Positive control substance:
- other: aminoacridine, 50 microgram/plate
- Remarks:
- for TA 1537 without S-9
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- For TA 98 without S-9
Migrated to IUCLID6: 2 microgram/plate
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- For TA 100 without S-9
Migrated to IUCLID6: 1 microgram/plate
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- Positive controls:
- yes
- Positive control substance:
- other: aminoanthracene, 1 microgram/plate
- Remarks:
- For all strains with S-9
- Details on test system and experimental conditions:
- Standard plate incorporation method. Two independent experiments are performed.
- Evaluation criteria:
- For a test to be valid, the bacteria have to meet the criteria for genotypes, the negative control plates should have a background lawn of growth, the spontaneous mutation rates should be within expected ranges, the positive controls should have induced the expected dose response and the sterility checks may not show contamination. Failure of one or more strain (or the S-9 mix) does not invalidate the remainder of the experiment. For a test compound to be scored as positive, one or both of the following criteria must be reproducibly met:
a) the compound must induce a dose-related increase in the number of revertant colonies
b) the compound must induce a doubling (or greater) in the number of revertants, at least at one dose level.
All compounds must be tested over a dose range covering at least two log intervals before being classed as negative. In the case of highly toxic compounds, care should be taken about the selection of doses around the toxic threshold. A biologically significant positive result in any tested strain is evidence of bacterial mutagenicity. - Statistics:
- Mean + standard deviation
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Cytotoxicity and mutagenic activity was not observed with 3-methylpyridine in the presence and absence of metabolic activation, according to OECD 471 bacterial reverse mutation assay guidelines.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.