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EC number: 946-324-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 October 2016 - 14 June 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- The study was conducted in accordance with international guidelines (OECD 202) and in accordance with GLP. All relevant guideline validity criteria were met.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- RADIOLABELLING INFORMATION (if applicable)
- Radiochemical purity: n/a
- Specific activity: n/a
- Locations of the label: n/a
- Expiration date of radiochemical substance: n/a
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: +6 ºC ± 2 ºC, protected from light and stored under nitrogen.
- Stability under test conditions: Assumed stable
- Solubility and stability of the test substance in the solvent/vehicle: n/a
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: n/a
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: no
- Preliminary purification step (if any): no
- Final dilution of a dissolved solid, stock liquid or gel: no
- Final preparation of a solid: n/a
FORM AS APPLIED IN THE TEST (if different from that of starting material): n/a
OTHER SPECIFICS: n/a - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 5.61, 17.9, 56.1, 179 and 561 µL of test item pipetted directly into Erlenmeyer test vessels containing 16 mL of synthetic waste water (prepared per OECD 209), 234 mL of demineralised water and 250 mL of innoculum to prepare nominal concnetrations of 10, 32, 100, 320 and 1000 mg/L.
- Eluate: Activated sewage sludge from the sewage plant at Hildesheim as it receives predominantly municipal sewage and hardly any industrial chemical waste.
- Differential loading: n/a
- Controls: Vessels prepared ad above but without test item.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): n/a
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): n/a
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): not reported - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: n/a
- Name and location of sewage treatment plant where inoculum was collected: Sewage plant at Hildesheim , Germany (no. 31137).
- Method of cultivation: n/a
- Preparation of inoculum for exposure: The sludge was washed twice with chlorine free tap water and adjusted to a dry sludge concentration of 3.0 g/L ± 10 %. The sludge was used within 24 h after sampling.
- Pretreatment: n/a
- Initial biomass concentration: Dry sludge contents = 2.84 g/L (1.42 g/L in the test vessels). - Test type:
- static
- Water media type:
- other: Activated sewage sludge
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 ºC
- pH:
- 7.5 ± 0.5
- Dissolved oxygen:
- 60 - 70 % saturation
- Nominal and measured concentrations:
- Nominal: 10, 32, 100, 320 and 1000 mg/L
- Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate (Merck, batch # A0382290) tested at 3 concentrations within the range of 58 - 180 mg/L and a dilution factor of 1.8. Solutions prepared from a 300 g/L stock and applied to vessels as per test treatment flasks.
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 61.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 264 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No
- Adsorption (e.g. of test material to the walls of the test container): Not reported
- Blank controls oxygen uptake rate: 33.2 - 37.2 mg O2/L/h
- Coefficient of variation of oxygen uptake rate in control replicates: 4.8 % - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: The EC50 for inhibition was 109 mg/L (95 % confidence limits = 107 - 112 mg/L) i.e. within the accepeted range of 53 - 155 mg/L.
- Other: No - Reported statistics and error estimates:
- EC10 95 % confidence limits = 49.2 - 74.2 mg/L.
EC50 95 % confidence limits = 216 - 324 mg/L. - Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC of the test item was 32 mg test item/L. The EC10 was 61.2 mg test item /L (95 % CI = 49.2 - 74.2 mg/L). The EC50 was 264 mg test item /L (95 % CI = 216 - 324 mg/L).
- Executive summary:
OECD 209 (2017) - A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item. The test system was activated sludge of the municipal treatment plant of 31137 Hildesheim, Germany. The test was carried out under static conditions with the test item concentrations; 10, 32, 100, 320 and 1000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates ranged from 0 % to 62 %.
In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50 value for the reference item was 109 mg/L, within guideline specifications.
The NOEC of the test item was 32 mg test item/L. The EC10 was 61.2 mg test item /L (95 % CI = 49.2 - 74.2 mg/L). The EC50 was 264 mg test item /L (95 % CI = 216 - 324 mg/L).
Reference
Table 1 Oxygen uptake rates and inhibition of the respiration of the control, test item and reference item solutions
Test Concentration (mg/L) |
Rep |
Oxygen Uptake Rate (mg O2/L/h) |
Inhibition (%) |
Mean Inhibition (%) |
Treatment vessels |
||||
Control |
1 |
36.4 |
- |
- |
2 |
37.2 |
- |
||
3 |
35.2 |
- |
||
4 |
33.6 |
- |
||
5 |
33.2 |
- |
||
6 |
33.6 |
- |
||
10 |
1 |
34.8 |
0 |
0 |
2 |
36.4 |
-4 |
||
3 |
34.4 |
1 |
||
4 |
34.0 |
3 |
||
5 |
35.2 |
-1 |
||
32 |
1 |
34.0 |
3 |
2 |
2 |
34.4 |
1 |
||
3 |
34.0 |
3 |
||
4 |
34.4 |
1 |
||
5 |
33.6 |
4 |
||
100 |
1 |
23.2 |
34 |
22 |
2 |
30.4 |
13 |
||
3 |
29.6 |
15 |
||
4 |
28.4 |
19 |
||
5 |
25.2 |
28 |
||
320 |
1 |
15.6 |
55 |
54 |
2 |
16.0 |
54 |
||
3 |
15.6 |
55 |
||
4 |
18.0 |
48 |
||
5 |
15.2 |
56 |
||
1000 |
1 |
14.0 |
60 |
62 |
2 |
13.2 |
62 |
||
3 |
12.4 |
64 |
||
4 |
12.8 |
63 |
||
5 |
12.8 |
63 |
||
Reference item |
||||
58 |
1 |
27.2 |
22 |
21 |
2 |
27.6 |
21 |
||
3 |
27.6 |
21 |
||
100 |
1 |
19.2 |
45 |
45 |
2 |
19.6 |
44 |
||
3 |
19.2 |
45 |
||
180 |
1 |
9.2 |
74 |
74 |
2 |
9.6 |
72 |
||
3 |
8.8 |
75 |
Table 2 Specific respiration rates of the control replicates
|
Rep |
Specific Respiration Rate (mg O2/g/h) |
Control |
1 |
25.6 |
2 |
26.2 |
|
3 |
24.8 |
|
4 |
23.7 |
|
5 |
23.4 |
|
6 |
23.7 |
|
Mean value |
24.6 |
Description of key information
3h EC50 = 264 mg/L. 3h EC10 = 61.2 mg/L; OECD 209; Anon. (2017)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 264 mg/L
- EC10 or NOEC for microorganisms:
- 61.2 mg/L
Additional information
A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item (Anon., 2017). The test system was activated sludge of the municipal treatment plant of 31137 Hildesheim, Germany. The test was carried out under static conditions with the test item concentrations; 10, 32, 100, 320 and 1000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates ranged from 0 % to 62 %.
In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50 value for the reference item was 109 mg/L, within guideline specifications.
The NOEC of the test item was 32 mg test item/L. The EC10 was 61.2 mg test item /L (95 % CI = 49.2 - 74.2 mg/L). The EC50 was 264 mg test item /L (95 % CI = 216 - 324 mg/L).
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