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EC number: 204-671-2 | CAS number: 124-02-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Salmonella Mutagenicity Tests: III. Results From the Testing of 255 Chemicals
- Author:
- Zeiger et al
- Year:
- 1 987
- Bibliographic source:
- Environmental Mutagenesis Volume 9, Supplement 9:1-110
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Diallylamine
- EC Number:
- 204-671-2
- EC Name:
- Diallylamine
- Cas Number:
- 124-02-7
- Molecular formula:
- C6H11N
- IUPAC Name:
- diallylamine
Constituent 1
- Specific details on test material used for the study:
- Purity: 98%
Method
- Target gene:
- histidine gene (for S. typhimurium)
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- liver S-9 from Aroclor-induced male Sprague-Dawley rats and Syrian hamsters
- Test concentrations with justification for top dose:
- 0, 33, 100, 333, 1000, 3333 and 10,000 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: ET95, ETOH; 95% ethanol (solvent)
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- ET95
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- sodium azide
- other: 4-nitro-o-phenylenediamine; 2-aminoanthracene
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 20 min.
- Exposure duration: 2 days
NUMBER OF REPLICATIONS: Tripicate (for each dose)
DETERMINATION OF CYTOTOXICITY
- Method: Number of his+ colonies and clearing in the density of background lawn. - Evaluation criteria:
- An individual trial was judged mutagenic (+) if a dose-related increase over the corresponding solvent control was seen, and it was judged weakly mutagenic (+W) if a low-level dose response was seen. A trial was considered questionable (?) if a dose-related increase was judged insufficiently high to justify a call of "+W" if only a single dose was elevated over the control, or if a non-dose related increase was seen.
A chemical was judged to be mutagenic (+), or weakly mutagenic (+W), if it produced a reproducible, dose-related increase in his+ revertants over the corresponding solvent controls in replicate trials. A chemical was considered to be questionable (?) if a reproducible increase of his+ revertants did not meet the criteria for either a "+" or "+W", or if only single doses produced an increase in his+ revertants in repeat trials.
Results and discussion
Test results
- Species / strain:
- other: S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- complete clearing of background lawn seen at 10000 µg/plate (TA100 and TA98) and at 3333 µg/plate (TA1537) without metabolic activation
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
Any other information on results incl. tables
A summary of results obtained for diallylamine are presented below:
Mutagenic responses of Salmonella tester strains (mean ± SEM; three plate) to Diallylamine. The 0 µg/plate dose is the solvent control.
Abbreviations:
ET95, ETOH; 95% ethanol (solvent)
POS: Positive control
NA: Not acivated
RLI: Aroclor 1254 -induced rat liver S-9.
HLI: Aroclor 1254 -induced hamster liver S-9
t: complete clearing of background lawn (colonies not counted)
(-): non-mutagenic
Diallyamine
Solvent: ET95
Dose |
TA100 |
TA1535 |
TA1537 |
TA98 |
||||||||||||||||||||
µg/plate |
NA (-) |
10% HLI (-) |
10% RLI (-) |
NA (-) |
10% HLI (-) |
10% RLI (-) |
NA (-) |
10% HLI (-) |
10% RLI (-) |
NA (-) |
10% HLI (-) |
10% RLI (-) |
||||||||||||
Mean |
SEM |
Mean |
SEM |
Mean |
SEM |
Mean |
SEM |
Mean |
SEM |
Mean |
SEM |
Mean |
SEM |
Mean |
SEM |
Mean |
SEM |
Mean |
SEM |
Mean |
SEM |
Mean |
SEM |
|
0 |
86 |
5.1 |
146 |
5.3 |
115 |
12.5 |
5 |
1.2 |
10 |
1.3 |
7 |
0.6 |
7 |
0.7 |
9 |
0.9 |
6 |
0.7 |
14 |
4.5 |
26 |
1.8 |
25 |
1.5 |
33 |
|
|
|
|
|
|
5 |
2.7 |
|
|
|
|
7 |
2.3 |
|
|
5 |
0.6 |
|
|
|
|
|
|
100 |
88 |
4.7 |
139 |
3.4 |
136 |
9.8 |
5 |
1.8 |
16 |
0.6 |
8 |
0.6 |
6 |
0.3 |
10 |
1.2 |
5 |
1.2 |
17 |
1.9 |
19 |
1.5 |
18 |
3.2 |
333 |
94 |
2.5 |
138 |
9.5 |
130 |
9.2 |
5 |
0.7 |
10 |
0.9 |
5 |
0.9 |
5 |
1.5 |
8 |
0.7 |
7 |
0.6 |
18 |
0.9 |
22 |
1.7 |
21 |
1.5 |
1000 |
88 |
2.4 |
145 |
16.0 |
145 |
3.8 |
2 |
1.9 |
7 |
2.4 |
6 |
1.0 |
6 |
1.5 |
8 |
0.9 |
5 |
2.3 |
20 |
2.0 |
21 |
4.5 |
18 |
1.2 |
3333 |
87 |
3.2 |
135 |
5.8 |
143 |
12.4 |
2 |
1.2 |
7 |
1.8 |
6 |
1.2 |
t |
|
5 |
0.9 |
2 |
0.6 |
7 |
3.1 |
15 |
4.1 |
17 |
2.6 |
10000 |
t |
|
132 |
5.2 |
120 |
11.8 |
2 |
0.5 |
3 |
0.5 |
2 |
0.7 |
|
|
1 |
0.3 |
|
|
t |
|
6 |
1.5 |
5 |
1.2 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
POS |
441 |
41.9 |
1955 |
23.9 |
1186 |
54.6 |
532 |
19.8 |
359 |
64.3 |
344 |
29.9 |
199 |
57.9 |
162 |
5.2 |
268 |
30.4 |
197 |
9.3 |
1421 |
69.0 |
741 |
125.3 |
Applicant's summary and conclusion
- Conclusions:
- Diallylamine was considered to be non-mutagenic under the conditions of this test.
- Executive summary:
Diallylamine was tested using a preincubation modification of the Salmonella/microsome test in the absence of exogenous metabolic activation and in the presence of liver S-9 from Aroclor induced male Sprague-Dawley rats and Syrian hamsters.
The tested Salmonella typhimurium strains were TA98, TA100, TA1535 and TA1537.
The test conceentrations of Diallylamine were 33, 100, 333, 1000, 3333 and 10,000 µg/plate, with concurrent solvent and positive controls.
Diallylamine was considered to be non-mutagenic under the conditions of this test. No reproducible, dose-related increase in his+ revertants over the corresponding solvent controls in replicate trials was observed.
The positive control chemicals used in thetest induced marked increases in the frequency of revertant colonies, both with and without metabolic activiation.
Complete clearing of background lawn was seen at 10000 µg/plate (TA100 and TA98) and at 3333 µg/plate (TA1537) without metabolic activation
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