Rhamnolipids: fermentation products of glucose with Pseudomonas putida, Pseudomonadaceae

Endpoint summary

Description of key information

The short-term screening-level data for aquatic toxicity determined EC50 (daphnids) > 196 mg/L. An acute toxicity study (OECD 203) test on the effects of Rhamnolipids: fermentation products of glucose with Pseudomonas bacteria on Rare Minnow was conducted. At the end of the test, the cumulative mortalities of the nominal concentration of 10.0 mg/L, 17.8 mg/L, 31. 8 mg/L, 56.2 mg/L and 100 mg/L test groups were 0%, 0%, 0% and 100%, respectively. Hence the effects of the test substance to Rare Minnow described as the 96 h median lethal concentration (LC50), the lowest concentration causing 100% mortality (LC100) and the highest concentration causing 0% mortality are: LC50 75 mg/mL, LC100 mg/L and LC0 56.2 mg/L. Thus, the LC50 (96h) is Rhamnolipids: fermentation products of glucose with Pseudomonas bacteria 75 mg/L. In addition, a guideline study on the toxicity of the substance to a freshwater alga (Pseudokirchneriella subcapitata) was conducted, and from which the 72 h ErC10 and 72 h ErC50 values were derived as >185 mg a.i./L.

A guideline study on acute toxicity of the substance on Daphnia magna was conducted and the value EC50 (48 h) >196 mg/L was derived. A semi-static reproduction test with Daphnia magna was conducted according to OECD 211 in order to investigate the chronic effects of the test item on daphnids. In the present study, five nominal concentrations (1.05, 3.36, 10.76, 34.42, and 110.13 mg test item (TI)/L, corresponding to 0.95, 3.05, 9.77, 31.25 and 100.0 mg active ingredient (a.i)./L (measured concentrations). The EC10 was determined to be 34.27 mg/L, based on the reproduction.