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EC number: 201-719-4 | CAS number: 87-03-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from Handbook.
Data source
Referenceopen allclose all
- Reference Type:
- review article or handbook
- Title:
- Acute oral toxicity of Tetrasodium 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonate] (72-57-1)
- Author:
- Richard J. Lewis, Sr.
- Year:
- 2 004
- Bibliographic source:
- Sax's Dangerous Properties of Industrial Materials. 11th Edition, p. 935, 2004.
- Reference Type:
- other: authoritative database
- Title:
- Acute oral toxicity of Tetrasodium 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonate] (72-57-1)
- Author:
- Hazardous Substances Data Bank
- Year:
- 2 017
- Bibliographic source:
- HSDB (Hazardous Substances Data Bank), US national Library of Medicine, 2017
- Reference Type:
- secondary source
- Title:
- Acute oral toxicity study prepared by food and Drug Research Laboratories Incorporated with cover letter
- Author:
- National Technical Reports Library
- Year:
- 1 972
- Bibliographic source:
- NTRL, Acute oral toxicity study prepared by food and Drug Research Laboratories Incorporated, OTS0215154, 1972
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as metioned below
- Principles of method if other than guideline:
- Acute oral toxicity of Tetrasodium 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonate] (CAS no: 72-57-1 ) in rat.
- GLP compliance:
- not specified
- Test type:
- other: not specified
- Limit test:
- no
Test material
- Reference substance name:
- Tetrasodium 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonate]
- EC Number:
- 200-786-7
- EC Name:
- Tetrasodium 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonate]
- Cas Number:
- 72-57-1
- Molecular formula:
- C34H28N6O14S4.4Na
- IUPAC Name:
- Tetrasodium 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonate]
- Details on test material:
- - IUPAC Name: Tetrasodium 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonate]
- Common Name: Trypan Blue
- Mol. formula: C34H24N6Na4O14S4
- Molecular Weight: 960.8156 g/mole
- Smiles: c12c(cc(c(c1O)/N=N/c1c(cc(cc1)c1cc(c(cc1)/N=N/c1c(cc3c(c1O)c(cc(c3)S(=O)(=O)[O-])N)S(=O)(=O)[O-])C)C)S(=O)(=O)[O-])cc(cc2N)S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+]
- InChI: 1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-37+,40-38+;;;;
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report):Niagara Blue 3B
- Molecular formula (if other than submission substance):C34H24N6Na4O14S4
- Molecular weight (if other than submission substance):960.8156 g/mole
- Substance type:Organic
- Physical state:Dark Blue Powder
PurityNo data
- Impurities (identity and concentrations):No data
Test animals
- Species:
- rat
- Strain:
- other: Sherman-Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: No data
- Age at study initiation: No data
- Weight at study initiation: No data
- Fasting period before study: 24 hours
- Housing: No data
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: one week
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 25% W/V Suspension in Water.
- Amount of vehicle (if gavage): No data
- Justification for choice of vehicle: No data
MAXIMUM DOSE VOLUME APPLIED:
- Rationale for the selection of the starting dose: - Doses:
- 4000, 5000, 6250 and 7900 mg/kg
- No. of animals per sex per dose:
- Total = 20 (Male/Female)
3 males and 2 females -4000 mg/kg
3 males and 2 females -5000 mg/kg
3 males and 2 females -6250 mg/kg
3 males and 2 females -7900 mg/kg - Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days
- Statistics:
- The mortality data were evaluated according to the Thompson Moving Average Method.
Results and discussion
- Preliminary study:
- not specified
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 6 200 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- > 5 500 - < 6 900
- Remarks on result:
- other: 50% mortality was observed
- Mortality:
- At 4000 mg/kg – All animals were survived.
At 5 000 mg/kg – All animals were survived.
At 6250 mg/kg – 3 animals were died out of 5
At 7900 mg/kg – 5 animals were died out of 5 - Clinical signs:
- other: not specified
- Gross pathology:
- not specified
- Other findings:
- not specified
Any other information on results incl. tables
Table – The acute oral toxicity in rats
Dosage level ml/kg |
Gm Equiv Dosage level gm/kg |
No. of Rats Dosed |
Day |
Mortality After 14 Days |
|||||||||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
||||
16.0 |
4.0 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0/5 |
20.0 |
5.0 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0/5 |
25.1 |
6.25 |
5 |
3 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
3/5 |
31.6 |
7.9 |
5 |
5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
5/5 |
Applicant's summary and conclusion
- Interpretation of results:
- Category 5 based on GHS criteria
- Conclusions:
- LD50 was considered to be 6200 mg/kg at 19/20 Confidence Limits of 5500 - 6900 mg/kg bw, when Sherman-Wistar rat was treated with Tetrasodium 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonate] orally.
- Executive summary:
The acute oral toxicity study was conducted by using Tetrasodium 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonate] in 4 groups of albino rats, consisting of 3 males and 2 females of the Sherman-Wistar rat at the concentration of 4000, 5000, 6250 and 7900 mg/kg. The test substance was in the form of Dark Blue Powder which dissolved at 25% W/V Suspension in Water (Vehicle). The animals were observed initially for a Acclimation period of 1 week to assure normalcy. The animals were then starved for 24 hours. Each animal administered given doses directly by gavage route. The animals were allowed food and water ad libitum during a 14 day observation period. The mortality data were evaluated according to the Thompson Moving Average Method. . The mortality data were evaluated according to the Thompson Moving Average Method. At 6250 gm/kg, 3 animals were died and at 7900 mg/kg all animals died. No mortality was observed at 4000, 5000 mg/kg. Therefore, LD50 was considered to be 6200 mg/kg at 19/20 Confidence Limits of 5500 - 6900 mg/kg bw, when Sherman-Wistar rat was treated with Tetrasodium 3,3'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonate] orally.
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