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EC number: 411-490-4 | CAS number: 7027-11-4 ISOPHORONENITRILE, IPN
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-01-05 to 1999-02-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.2 (Acute Toxicity (Inhalation))
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1300 (Acute inhalation toxicity)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 3-cyano-3,5,5-trimethylcyclohexanone
- EC Number:
- 411-490-4
- EC Name:
- 3-cyano-3,5,5-trimethylcyclohexanone
- Cas Number:
- 7027-11-4
- Molecular formula:
- C10H15NO
- IUPAC Name:
- 3-cyano-3,5,5-trimethylcyclohexanone
- Details on test material:
- Isophorone nitrile of Creanova (former Hüls, later Degussa) from continous production on 16 Nov 1998.
Purity 99.6 % (GC)
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ORGANISMS:
- Strain: HanIbm:WIST(SPF) = HanBrl:WIST(SPF)
- Source: RCC Ltd., Biotechnology & Animal Breeding Division, Füllinsdorf (CH)
- Age: 9-10 weeks
- Weight at study initiation: males 210.6-238.1 g,females 193.7-224.0 g
- Number of animals: 5 per dose and gender
- Housing: groups of five
- Diet: ad libitum, rat maintenance diet Kliba 3433
- Water: ad libitum, tab water
- Acclimation period: 5 to 6 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20°C +/- 1 °C
- Humidity (%): 43 - 66 %
- Air changes (per hr): 10 - 15 per hour
- Photoperiod (hrs dark / hrs light): 12 hours darkness, 12 hours artifical light
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- nose only
- Vehicle:
- other: compressed filtered air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Type of exposure: nose-only, flow-past according to Sachsse et al. (1973, 1976)
- Method of holding animals in test chamber: separatley in restraint tubes, positioned radially around the exposure chamber
- Source and rate of air: compressed filtered air, 5.0 - 5.6 l/min
- Method of conditioning air: calibrated pressure gauge and flow meter
- Type or preparation of particles: Warming to 76-88 °C in a polyethyleneglycol 400 bath followed by aerosol generation with a glass
nebulizer with stainless steel nozzle and inlet
- Method of particle size determination: gravimetrically determination using a Mercer 7 stage cascade impactor
- Temperature, humidity: T: 22.1 - 22.6°C, H: 8.0 - 11.2 % RH,
TEST ATMOSPHERE
- Concentrations: 291; 1554; 3566 mg/m3 nominal (i.e. decrease in weight of test material divided by airflow volume)
256 +/- 17 (n=5); 979 +/- 50 (n=10); 3031 +/- 272 (n=9) mg/m3 analytical (using glass fiber filters)
- Brief description of analytical method used: Gravimetric determination of aerosol concentrations, samples from test atmosphere were collected
during the exposure (5/Group 1, 10/Group 2, 9/Group 3) on Gelman glass fiber filters, loaded in a 47 mm inline stainless steel filter sampling
device (Gelman Science Inc.)
- Particle size: in order of increasing test concentration mass median aerodynamic diameters 2.86; 2.21; 1.70 µm
geometric standard deviations 3.05 (n=3); 2.33 (n=2); 1.81 (n=2)
- Samples taken from breathing zone: yes, from an empty port of the exposure chamber - Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- Gravimetric determination of aerosol concentrations. In view of the fairly low vapor pressure and high purity of the test article, determination of aerosol concentrations by chemival analysis were not deemed necessary.
- Duration of exposure:
- 4 h
- Concentrations:
- 256 +/- 17; 979 +/- 50; 3031 +/- 272 mg/m3
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- EXAMINATIONS:
- Post dose observation period: 14 days
- body weights: before and 3, 7, and 14 days after treatment mortality: once per hour during, and once after treatment on day of exposure;
thereafter twice daily
- clinical signs: three times during exposure, at end of exposure, thereafter once daily including day of treatment, including: changes in behavior,
somatomotor activity, body position, respiratory and circulatory effects, autonomic effects, central nervous system effects, reactivity to handling
or sensory stimuli, altered strength, alteration of skin, fur, nose, and eyes.
- Necropsy: all animals (macroscopic) when found dead or at terminal sacrifice - Statistics:
- LOGIT-Model (Program for Dosa-Response Analysis) was used to evaluate the toxicity of isophorone nitril and to calculate the LC50 value with its
95 % confidence limits
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- 1.34 mg/L air
- 95% CL:
- 0.84 - 2.75
- Exp. duration:
- 4 h
- Remarks on result:
- other: no remarks
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- 1.4 mg/L air
- 95% CL:
- 0.66 - 6.82
- Exp. duration:
- 4 h
- Remarks on result:
- other: no remarks
- Sex:
- female
- Dose descriptor:
- LC50
- Effect level:
- 1.2 mg/L air
- 95% CL:
- 0.59 - 4.02
- Exp. duration:
- 4 h
- Remarks on result:
- other: no remarks
- Mortality:
- MORTALITY:- Number of deaths at each dose:
256 mg/m3: no deaths
979 mg/m3: 0 males, 1 female dead within 48 minutes after end of exposure
3031 mg/m3: 5 males, 5 females dead within 3 hours 24 minutes after beginning of exposure - Clinical signs:
- other: CLINICAL SIGNS: Salivation (256 and 979 mg/m3); effects on breathing (bradypnea and/or tachypnea; 979 and 3031 mg/m3); ruffled fur and behavioral effects (restlessness and decreased spontaneous activity; 979 mg/m3); 1 male and 1 female with red skin/f
- Body weight:
- BODY WEIGHT: Body weight gain was transiently inhibited only in surviving females at 979 mg/m3.
- Gross pathology:
- NECROPSY FINDINGS: There were no macroscopical pathology findings which could be attributed to treatment with the test article.
- Other findings:
- no other findings
Any other information on results incl. tables
no further remarks
Applicant's summary and conclusion
- Interpretation of results:
- harmful
- Remarks:
- Migrated information Criteria used for interpretation of results: other: EU Directive 67/548/EEC
- Conclusions:
- The LC 50 of isophorone nitrile for acute inalation toxicity (4 h) was estimated to be 1.34 mg/l air. The no toxic level was 0.256 mg/l air whist one of ten animals died at 0.979 mg/l air and all animals at 3.031 mg/l air.
- Executive summary:
The aim of this acute 4-hour inhalation study was to assess the acute inhaltion toxicity of isophorone nitrile when administered to rats for a single continuous 4-hour period followed by an observation period of 15 days. 3 groups of five male and five female Wistar rats were exposed by nose only, flow past inhaltion with concentrations of 0.256; 0.979 and 3.031 mg/l air. Principal clinical signs were salivation seen at 0.256 and 0.979 mg/l air, effects on breathing (bradypnea and/or tachypnea) at 0.979 ans 3.031 mg/l air, and behavioural effects (restlessness and decreased spontaneous activity) and ruffled fur at 0.979 mg/l air. Body weight development was not adversely affected in both genders at 0.256 mg/l air and in males at 0.979 mg/l air. A transient slight loss of body weight was seen in the surviving females at 0.979 mg/l air. There were no macroscopical pathology findings which could be attributed to treatment with the test article. The LC 50 of isophorone nitrile for acute inalation toxicity (4 h) was estimated to be 1.34 mg/l air. The no toxic level was 0.256 mg/l air whist one of ten animals died at 0.979 mg/l air and all animals at 3.031 mg/l air.
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