Butanoic acid, 4-[[4-[7-chloro-6-(1,1-dimethylethyl)-3H-pyrazolo[1,5-b][1,2,4]triazol-2-yl]phenyl]amino]-4-oxo-, tetradecyl ester

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

30 September 2003 to 11 October 2003

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

GLP compliance:

yes

Type of method:

column elution method

Key result

Water solubility:

< 0.05 mg/L

Conc. based on:

test mat.

Temp.:

25 °C

pH:

>= 6.59 - <= 7.45

Details on results:

The chromatograms obtained with 10 µL injection volumes at 292 nm exhibited an instrument detection limit of 0.05 mg/L based on an instrument detection limit standard at a prepared concetration of 0.05 mg/L. Samples A-1/B-1 to A-5/B-5 did not exhibit absorptions for the test substance. The blank water samples (A and B) did not exhibit absorptions for the test substance.

The Instrument Detection Limit was 0.05 mg/L based on an analyzed standard.

Time (hours)	Sample ID	pH	System A/System B Conc. (mg/L)
system blank	A/B	7.18/7.43	ND/ND
45	A-1/B-1	6.59/6.87	ND/ND
47	A-2/B-2	7.15/6.85	ND/ND
51	A-3/B-3	6.94/7.07	ND/ND
93	A-4/B-4	7.06/7.45	ND/ND
96	A-5/B-5	6.82/6.93	ND/ND

ND - 'None detected'. The test system chromatograms had no observable test substance peak above the instrument detection limit of 0.05 mg/L.

Conclusions:

Interpretation of results: insoluble (< 0.1 mg/L)
Under the conditions of the test, the water solubility of the test substance was less than 0.05 mg/L (based on the analyzed instrument detection limit test substance standard).

Executive summary:

In a GLP compliant water solubility study conducted in line with OECD Guideline 115 and EU Method A.6, the water solubility of butanoic acid, 4-[[4-[7-chloro-6-(1,1-dimethylethyl)-3H-pyrazolo[1,5-b][1,2,4]triazol-2-yl]phenyl]amino]-4-oxo-, tetradecyl ester was determined using the column elution method. The water solubility of the test substance was determined to be less than 0.05 mg/L (based on the analyzed instrument detection limit test substance standard).

Description of key information

Key study:- Isaacs (2004) 'Water solubility-column elution' conducted in line with OECD Guideline 105 and EU Method A.6. The water solubility of the test substance was determined to be <0.05 mg/L

Additional information

The key study (Isaacs, 2004) was performed in compliance with GLP and to the OECD Guideline 105 and EU Method A.6 with a sufficient level of detail to assess the quality of the presented data. The study was performed to a good standard in line with accepted, standardised guidelines and was assigned a reliability score of 1 using the principles for assessing data quality as set out in Klimisch et al. (1997). The water solubility of the test substance was determined to be <0.05 mg/L (based on the analyzed instrument detection limit test substance standard).