Isobutyl vinyl ether

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

no

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Isobutyl vinyl ether

EC Number:

203-678-8

EC Name:

Isobutyl vinyl ether

Cas Number:

109-53-5

Molecular formula:

C6H12O

IUPAC Name:

1-(ethenyloxy)-2-methylpropane

Details on test material:

isobutyl vinyl ether (IBVE)
purity > 99.5%
Stability was given during study period

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

Mean body weights: 267 and 183 g for male and female rats, respectively
Age: 8 to 9 weeks at start of treatment
Rats received standard diets and water ad libitum
Room temperature was 20-24°C
Relative air humidity 30-70%.

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: air

Details on inhalation exposure:

The animals were singly placed into compartments in an inhalation chamber (glass-steel, volume 200 l). Atmosphere was dynamically generated. Air passed an evaporator to which TS was pumped. Vapors were mixed with fresh air. Air was delivered to the inhalation chamber at a rate of 3000 l/h.

Analytical verification of test atmosphere concentrations:

yes

Remarks:

(the concentration was measured using gas chromatography)

Duration of exposure:

4 h

Concentrations:

21.1 mg/L

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

Post exposure period was 14 days.
The animals were observed for clinical signs of toxicity on working days and daily for mortality for 14 days. Body weights were recorded on days 0, 7, and 14. At the end of the observation period the animals were sacrificed and necropsied.

Statistics:

Binominal test (Wittig 1974) was used.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC0

Effect level:

21.1 mg/L air (analytical)

Exp. duration:

4 h

Remarks on result:

other: clinical signs but no mortality, 14 d observation period

Mortality:

no mortality

Clinical signs:

other: During exposure: apathia, stagger, depressed breathing, reddened ears and extremities, unkempt fur. After exposure: unsteady gait. No symptoms were noted from the day following exposure.

Body weight:

Body weight development was comparable to historical controls but slightly delayed in females.

Gross pathology:

No effects detected at necropsy.

Other findings:

no

Applicant's summary and conclusion

Conclusions:

In an acute inhalation study in rats no mortality was found after 4 h exposure to 21.1 mg/L and a post exposure period of 14 days (the recommended limit dose according to OECD TG403 is 5 mg/L); LC50 (4h) > 21 mg/L.