Registration Dossier
Registration Dossier
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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 201-732-5 | CAS number: 87-22-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
- Justification for type of information:
- Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Toxicity to micro-organisms study was conducted for 24 hrs using five different organisms such as Corynebacterium minutissimum, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermis, Arthrobacter spp., respectively.
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Vehicle:
- yes
- Remarks:
- ethyl alcohol or DMSO was used as a vehicle.
- Details on test solutions:
- - Sampling method: Test chemical solutions were prepared in either ethyl alcohol or DMSO depending on test chemical solubility.
- Test organisms (species):
- other: Corynebacterium minutissimum, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermis, Arthrobacter sp.
- Details on inoculum:
- - Laboratory culture: Staphylococcus aureus (IAM-1011, SA) was purchased from Institute of Applied Microbiology, Tokyo university. Escherichia coli (ATCC 11775, (EC)) was purchased from Institute of Medical Science, Tokyo university. Corynebacterium minutissimum (ATCC 23348, (CM)) and Staphylococcus epidermis var. (SE) were gifted from the Department of Dermatology, University of Pennsyvania. Arthrobacter sp. was isolated from Lipo-66.
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 24 h
- Test temperature:
- 37°C
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Culture dish was used as a test vessel.
- Material, size, headspace, fill volume: Culture dish of 35 X 10 mm dimension was used.
TEST MEDIUM / WATER PARAMETERS: Muller Hinton agar was used as a test medium.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Minimum inhibitory concentration (MIC) was determined as the concentration where no growth of the test organism was observed after 24 hrs.
- Reference substance (positive control):
- no
- Key result
- Duration:
- 24 h
- Dose descriptor:
- other: MIC
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on the effect of test chemical on the growth inhibition of five different organisms such as Corynebacterium minutissimum, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermis, Arthrobacter spp. after the exposure period of 24 hours, the MIC value was determined to be > 2000 mg/l.
- Executive summary:
Toxicity to micro-organisms study was conducted using five different microorganisms.The study was performed for 24 hrs at 37°C. Test organism Staphylococcus aureus (IAM-1011, SA) was purchased from Institute of Applied Microbiology, Tokyo university. Escherichia coli (ATCC 11775, (EC)) was purchased from Institute of Medical Science, Tokyo university.Corynebacterium minutissimum (ATCC 23348, (CM)) and Staphylococcus epidermis var.(SE) were gifted from the Department of Dermatology, University of Pennsyvania. Arthrobacter sp. was isolated from Lipo-66. Culture dish of 35 X 10 mm dimension was used as a test vessel. Test chemical solutions were prepared in either ethyl alcohol or DMSO. Muller Hinton agar was used as a test medium. Test bacteria were pre-propagated with sensitivity test broth of NISSUI using shaking culture. Incubated mediums were diluted using 0.75% physiological saline to the microbial concentration of 10E6 CFU/ml. Test medium containing the test chemical was inoculated using 0.1 ml of diluted culture solution. MIC was determined after 24 hrs at 37°C. Based on the effect of test chemical on the growth inhibition of five different organisms such as Corynebacterium minutissimum, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermis, Arthrobacter spp. after the exposure period of 24 hours, the MIC value was determined to be > 2000 mg/l.
Reference
Description of key information
Toxicity to micro-organisms study was conducted using five different microorganisms. The study was performed for 24 hrs at 37°C. Test organism Staphylococcus aureus (IAM-1011, SA) was purchased from Institute of Applied Microbiology, Tokyo university. Escherichia coli (ATCC 11775, (EC)) was purchased from Institute of Medical Science, Tokyo university. Corynebacterium minutissimum (ATCC 23348, (CM)) and Staphylococcus epidermis var.(SE) were gifted from the Department of Dermatology, University of Pennsyvania. Arthrobacter sp. was isolated from Lipo-66. Culture dish of 35 X 10 mm dimension was used as a test vessel. Test chemical solutions were prepared in either ethyl alcohol or DMSO. Muller Hinton agar was used as a test medium. Test bacteria were pre-propagated with sensitivity test broth of NISSUI using shaking culture. Incubated mediums were diluted using 0.75% physiological saline to the microbial concentration of 10e6 CFU/ml. Test medium containing the test chemical was inoculated using 0.1 ml of diluted culture solution. MIC was determined after 24 hrs at 37°C. Based on the effect of test chemical on the growth inhibition of five different organisms such as Corynebacterium minutissimum, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermis, Arthrobacter spp. after the exposure period of 24 hours, the MIC value was determined to be > 2000 mg/l.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 2 000 mg/L
Additional information
Toxicity to micro-organisms study was conducted using five different microorganisms. The study was performed for 24 hrs at 37°C. Test organism Staphylococcus aureus (IAM-1011, SA) was purchased from Institute of Applied Microbiology, Tokyo university. Escherichia coli (ATCC 11775, (EC)) was purchased from Institute of Medical Science, Tokyo university. Corynebacterium minutissimum (ATCC 23348, (CM)) and Staphylococcus epidermis var.(SE) were gifted from the Department of Dermatology, University of Pennsyvania. Arthrobacter sp. was isolated from Lipo-66. Culture dish of 35 X 10 mm dimension was used as a test vessel. Test chemical solutions were prepared in either ethyl alcohol or DMSO. Muller Hinton agar was used as a test medium. Test bacteria were pre-propagated with sensitivity test broth of NISSUI using shaking culture. Incubated mediums were diluted using 0.75% physiological saline to the microbial concentration of 10e6 CFU/ml. Test medium containing the test chemical was inoculated using 0.1 ml of diluted culture solution. MIC was determined after 24 hrs at 37°C. Based on the effect of test chemical on the growth inhibition of five different organisms such as Corynebacterium minutissimum, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermis, Arthrobacter spp. after the exposure period of 24 hours, the MIC value was determined to be > 2000 mg/l.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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