Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 228-589-1 | CAS number: 6300-50-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 31/05/2017 - 03/07/2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: The method described in Rev. Toxicol. 22 (2005) 169-174 (Coeficientes de partición de hidrocarburos aromáticos policíclicos con sustancias húmicas: un método simple para su determinación)
- Principles of method if other than guideline:
- - Principle of test as described in the reference article: Humic substances are major components of natural water. They are acidic polymers, mostly aromatic and usually harmless. However, they play an important role because they affect bioavailability and toxicity of many compounds in aquatic environments. Polycyclic aromatic hydrocarbons (some of them carcinogenic) are considered to be some of the most common pollutants of aquatic systems. They are usually found in the environment as complex mixtures exhibiting a wide range of physical, chemical and toxicological properties. In the present work, the interaction between humic substances and phenantrene and anthracene, individually or in mixtures,was studied. Partition coefficients of the substances between dissolved organic carbon and liquid phase were estimated by a simple methodology involving solid phase extraction and UV/VIS spectrophotometry. Koc values obtained by this means are comparable with those obtained using other methods.
The method described is a valuable tool for analyzing interactions of hydrophobic pollutants with humic substances.
- Short description of test conditions: Partition coefficients of the substances between dissolved organic carbon and liquid phase were estimated by a simple methodology involving solid phase extraction and HPLC measurements.
- Parameters analysed / observed: concentration of the substance in the humic media. - GLP compliance:
- no
- Remarks:
- Test done for screening purposes
- Type of method:
- HPLC estimation method
- Media:
- other: humic substance
- Radiolabelling:
- no
- Details on study design: HPLC method:
- EQUIPMENT
- Apparatus:
Liquid chromatograph: Agilent 1100 (Agilent Technologies)
Balance: Mettler Toledo AT261 Delta Range.
Visiprep Solid Phase Extraction Vacuum Manifolds (SUPELCO).
Software: Chemstation (Agilent Technologies).
- Type, material and dimension of analytical (guard) column: Column: Xterra RP C18 5 flm, 3.9 x 150mm (Waters).
- Detection system: PDA detector
SAMPLE PREPARATION:
Preparation of stock solutions: 10.75 mg of sample was accurately weighed and dissolved in 100.0 mL of ultrapure deionized water in a volumetric flask. The concentration of dye in the solution was 107.5 mg/L.
Standard solutions of dissolved humic substance (DHS) were prepared by dissolving 20.30 mg of Humic acid (Aldrich, Lot BCBS 1775V) in 100.0 mL of Milli Q water in a volumetric flask and after stirring for one hour on a magnetic stirrer. The solution was filtered through 0.45 flm nylon membrane.
The theoretical concentration of dissolved humic substance was 203.00 mg/L. The total organic carbon in the humic acid reference substance, determined byelemental organic analysis was 43.43%.
INTERACTION ANALYTICAL SUBSTANCES PREPARATION:
Interaction samples between the dye and the dissolved humic substance were prepared by increasing the dissolved humic substance while keeping constant the concentration of dye. Three different interaction samples (in duplicate) were prepared in Milli Q water at 3 levels of concentration DHS: 14.21,21.32 and 42.63 mg/L (corresponding to 6.17, 9.26 and 18.51 mglL of TOC). The concentration of colorant in all interaction samples was 2.002 mg/L.
EXTRACTION METHOD BY SOLID PHASE EXTRACTION:
In order to separate the free dye from interaction samples DHS-dye in the aquous mixture according to their polarity properties, a solid phase extraction was performed on a cartridge Discovery DSC-18 100 mg/1 mL (Supelco). The free dye was retained on the stationary phase and the polar fraction from interaction samples DHS-dye passed throught the caIiridge. Liquid extract containing the component of interest was injected in the chromatographic system.
All stock solutions and interaction analytical samples were stored in a refrigerator.
MOBILE PHASES
- Type:
A: H20 Milli-Q (9:1), 0.02 M Tetrabutylammonium bromide, 0.1 M KH2P04, pH 6.5 with NaOH 2N.
B: H20 Milli-Q (46.6:53.3), 0.018 M Tetrabutylammonium bromide, 0.092 M KH2P04, pH 6.5 with NaOH 2N.
Gradient:
Time (m in) 0 Solvent A (%) 25 Solvent B (%) 75
Time (m in) 10 Solvent A (%) 0 Solvent B (%) 100
Time (m in) 20 Solvent A (%) 0 Solvent B (%) 100
Time (m in) 28 Solvent A (%) 25 Solvent B (%) 75
Time (m in) 35 Solvent A (%) 25 Solvent B (%) 75
Injection: 50 µL
Flow rate: 1.0 mL/min
Detection: 515 nm
- Solutes for dissolving test and reference substances: water
REPETITIONS
- Number of determinations: 2
EVALUATION
- Determination of the log Koc value: calculation from Koc value determined in the experiment - Key result
- Type:
- Koc
- Value:
- 1 330 000 L/kg
- Temp.:
- 25 °C
- Key result
- Type:
- log Koc
- Value:
- 6.12 dimensionless
- Temp.:
- 25 °C
- Validity criteria fulfilled:
- yes
- Conclusions:
- The experimental Koc value obtained for the test item is 1,33X10E6
Reference
Description of key information
The Koc and log Koc have been determined experimentally for this substance. The value of log Koc > 5 indicates that the adsorption to soil is likely to be high.
Key value for chemical safety assessment
- Koc at 20 °C:
- 1 330 000
Additional information
[LogKoc: 6.12]
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.