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EC number: 265-747-9 | CAS number: 65405-84-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- February 17th, 2017 to March 27th, 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- α,2,6,6-tetramethyl-2-cyclohexene-1-butyraldehyde
- EC Number:
- 277-458-5
- EC Name:
- α,2,6,6-tetramethyl-2-cyclohexene-1-butyraldehyde
- Cas Number:
- 73398-85-3
- Molecular formula:
- C14H24O
- IUPAC Name:
- 2-methyl-4-(2,6,6-trimethylcyclohex-2-en-1-yl)butanal
- Reference substance name:
- α,2,6,6-tetramethylcyclohexene-1-butyraldehyde
- EC Number:
- 282-993-2
- EC Name:
- α,2,6,6-tetramethylcyclohexene-1-butyraldehyde
- Cas Number:
- 84518-22-9
- Molecular formula:
- C14H24O
- IUPAC Name:
- 2-methyl-4-(2,6,6-trimethylcyclohex-1-en-1-yl)butanal
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- Test material name (as stated in the report): Cetonal
Batch No.: SC00019822
Expiry date: 01 March 2019
In vitro test system
- Test system:
- human skin model
- Remarks:
- EPISKIN Small ModelTM
- Source species:
- human
- Cell type:
- other: EPISKIN-SMTM, 0.38 cm2
- Cell source:
- other: SkinEthic Laboratories, Lyon, France.
- Details on animal used as source of test system:
- This model is a three-dimensional human epidermis model, which consists of adult human-derived epidermal keratinocytes which have been seeded on a dermal substitute consisting of a collagen type I matrix coated with type IV collagen. The keratinocytes were cultured for 13 days, which results in a highly differentiated and stratified epidermis model comprising the main basal, supra basal, spinous and granular layers and a functional stratum corneum.
- Justification for test system used:
- In the interest of sound science and animal welfare, a sequential testing strategy is recommended to minimize the need of in vivo testing. One of the validated in vitro skin irritation tests is the EPISKIN test, which is recommended in international guidelines (e.g. OECD and EC).
- Vehicle:
- unchanged (no vehicle)
- Remarks:
- The liquid test item was applied undiluted (25 µl) directly on top of the tissue
- Control samples:
- yes, concurrent negative control
- yes, concurrent positive control
- Duration of treatment / exposure:
- CETONAL was applied undiluted (25 µl) directly on top of the skin tissue for 15 ± 0.5 minutes.
- Duration of post-treatment incubation (if applicable):
- After a 42 hour post-incubation period, determination of the cytotoxic (irritancy) effect was performed. Cytotoxicity is expressed as the reduction of mitochondrial dehydrogenase activity measured by formazan production from MTT at the end of the treatment.
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- % tissue viability
- Run / experiment:
- second test after treatment with CETONAL
- Value:
- 33
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
The positive control had a mean cell viability of 12% after 15 ± 0.5 minutes exposure.
The absolute mean OD570(optical density at 570 nm) of the negative control tissues was within the laboratory historical control data range. The standard deviation value of the percentage viability of three tissues treated identically with the control items was less than 7%, indicating that the test system functioned properly.
The relative mean tissue viability obtained after 15 ± 0.5 minutes treatment with CETONAL compared to the negative control tissues was 57% (individual values 38%, 100% and 33%). However, the standard deviation value between tissue replicates of CETONAL was 37% after 15 minutes exposure which is not within the acceptability criteria of the assay (≤18%). Therefore the test was repeated.
The relative mean tissue viability obtained in the second test after 15 ± 0.5 minutes treatment with CETONAL compared to the negative control tissues was 43% (individual values 59%, 44% and 26%).
The relative mean tissue viability obtained in the second test after treatment with CETONAL compared to the second set of negative control tissues with a standard deviation of 11% was 33% (individual values 46%, 34% and 20%).
The positive control had a mean cell viability of 7.3% after 15 ± 0.5 minutes exposure.
Since the mean relative tissue viability for CETONAL was below 50% after 15 ± 0.5 minutes treatment in 4 out of 5 tissues, CETONAL is considered to be irritant.
Applicant's summary and conclusion
- Interpretation of results:
- Category 2 (irritant) based on GHS criteria
- Conclusions:
- In conclusion, CETONAL is irritant in the in vitro skin irritation test under the experimental conditions described in this report.
- Executive summary:
The objective of this study was to evaluate CETONAL for its ability to induce skin irritation on a human three dimensional epidermal model (EPISKIN Small model (EPISKIN-SMTM)). The possible skin irritation potential of CETONAL was tested through topical application for 15 minutes. The study procedures described in this report were based on the most recent OECD and EC guidelines.
Batch SC00019822 of CETONAL was a pale yellow liquid with a purity of 98.02%. CETONAL was applied undiluted (25 µl) directly on top of the skin tissue for 15 ± 0.5 minutes. After a 42 hour post-incubation period, determination of the cytotoxic (irritancy) effect was performed. Cytotoxicity is expressed as the reduction of mitochondrial dehydrogenase activity measured by formazan production from MTT at the end of the treatment.
Skin irritation is expressed as the remaining cell viability after exposure.
The positive control had a mean cell viability of 12% after 15 ± 0.5 minutes exposure.
The absolute mean OD570(optical density at 570 nm) of the negative control tissues was within the laboratory historical control data range.
The standard deviation value of the percentage viability of three tissues treated identically with the control items was less than 7%, indicating that the test system functioned properly.
The relative mean tissue viability obtained after 15 ± 0.5 minutes treatment with CETONAL compared to the negative control tissues was 57% (individual values 38%, 100% and 33%). However, the standard deviation value between tissue replicates of CETONAL was 37% after 15 minutes exposure which is not within the acceptability criteria of the assay (≤18%). Therefore the test was repeated.
The relative mean tissue viability obtained in the second test after 15 ± 0.5 minutes treatment with CETONAL compared to the negative control tissues was 43% (individual values 59%, 44% and 26%).
The relative mean tissue viability obtained in the second test after treatment with CETONAL compared to the second set of negative control tissues with a standard deviation of 11% was 33% (individual values 46%, 34% and 20%).
The positive control had a mean cell viability of 7.3% after 15 ± 0.5 minutes exposure.
Since the mean relative tissue viability for CETONAL was below 50% after 15 ± 0.5 minutes treatment in 4 out of 5 tissues, CETONAL is considered to be irritant.
In conclusion, CETONAL is irritant in the in vitro skin irritation test under the experimental conditions described in this report.
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