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EC number: 478-250-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 478-250-9
- EC Name:
- -
- Cas Number:
- 62965-37-1
- Molecular formula:
- C26H42N2O4
- IUPAC Name:
- (2S)-2-{[(benzyloxy)carbonyl]amino}-3,3-dimethylbutanoic acid; N-cyclohexylcyclohexanamine
Constituent 1
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- The Result of a preliminary solubility trial showed that VRT-126017 dcha was insufficiently soluble to allow the preparation of a suitable aqueous
stock solution for either test. Therefore, at test initiation appropriate weights were established in one-litre test beakers, dechlorinated tap water
(284 ml) was added and the mixtures then stirred on a magnetic stirrer for one hour and twenty five minutes in order to form dispersions.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- A sample of activated sludge was obtained the day before the start of the tests from the aeration basin at Worlingworth Sewage Treatment Works,
which treats predominantly domestic waste. In the laboratory, the samples were maintained under aerobic conditions until required. The
concentration of suspended solids in a homogenised sample was determined on the day of collection and immediately before the start of the tests.
On the day of collection, aliquots (25 ml) of the activated sludge were filtered through dried and preweighed Whatman GF/C filter papers which were
then dried again at approximately 105°C for at least one hour, allowed to cool in a desiccator and reweighed. The mixed liquor suspended solids
(MLSS) content of the activated sludge was then calculated. In the definitive test the MLSS was adjusted to 4 g/l by the removal of supernatant.
Synthetic sewage (50 ml/l) was added to each stock of activated sludge and these were aerated overnight. On the day of the test, the MLSS content of
the sludge was determined and adjusted to 4 g/l by the addition of dechlorinated tap water. The pH and temperature of the sludge was also measured.
Study design
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- 200-250 mg/L as CaCO3
- Nominal and measured concentrations:
- preliminary range finding test at nominal concentrations of 10, 100 and 1000 mg/l
second (definitive) test at nominal concentrations of 500, 1000, 2000, 4000 and 8000 mg/l - Details on test conditions:
- The prepared mixtures were aerated for three hours in a thermostatically-controlled water bath, using a glass aerator connected to a laboratory
supply of oil-free compressed air (one litre/minute).Following the exposure period, a well-mixed sample of each mixture was transferred to a
biochemical oxygen demand (BOD) bottle (nominal capacity, 270 ml). The rate of oxygen consumption was measured, over a period of approximately ten minutes or until the dissolved oxygen concentration fell below 2 mgO2/l using a Yellow Springs Instrument (YSI) dissolved oxygen meter, with
temperature probe and self stirring bottle probe, connected to a chart recorder. The pH and temperature of the samples were measured at the start
and end of the test. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The EC50 of the test substance was calculated from the results of the definitive test to be 4320 mg/l (95% confidence limits, 2760 - 7900 mg/l). The
EC20 value was calculated to be 703 mg/l (95% confidence limits, 231 - 1500 mg/l). - Results with reference substance (positive control):
- Sludge respiration rates were progressively reduced in the presence of increasing concentrations of 3,5-DCP. The three-hour 50% effect
concentrations (EC50) for 3,5-DCP was calculated to be 16.3 mg/l (95% confidence limits, 9.5 - 34.7 mg/l) in the preliminary test, and 12.0 mg/l (95% confidence limits, 7.4 - 20.0 mg/l) in the definitive test.
Applicant's summary and conclusion
- Conclusions:
- The EC50 of the test substance was calculated to be 4320 mg/l (95% confidence limits, 2760 - 7900 mg/l). The EC20 value was calculated to be 703 mg/l (95% confidence limits, 231 - 1500 mg/l).
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