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EC number: 214-684-5 | CAS number: 1185-53-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jan 18 - Mar 07, 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Principles of method if other than guideline:
- none
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hessisches Ministerium für Umwelt, Landwirtschaft und Forsten
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1000 mg lest item were dissolved in 200 mL deionized water. Thus, a concentration of 5 .0 g/L was obtained.- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Species: activated sludge, micro organisms from a domestic waste water treatment plant.
Origin: supplied by the sewage plant Groß Zimmern, Germany - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- 250 mg/L
- Test temperature:
- 19 - 20 °C
- pH:
- saturated (7-8 mg/L)
- Dissolved oxygen:
- 7.7 - 9.1 mg/L
- Nominal and measured concentrations:
- 0, 10, 32, 100, 320, 1000 mg/L nominal
- Details on test conditions:
- Measurement of Respiration Rate:
For the measurement of the respiration rate a well-mixed sample of each test medium was poured into a Karlsruher flask after exactly 3 hours incubation time, and was not further aerated. The oxygen concentration was measured with an oxygen electrode, and was recorded for about ten minutes. During measurement, the samples were continuously stirred on a magnetic stirrer.
The oxygen consumption (in mg 02 per L per minute) was determined from the most linear part of the respiration curve. - Reference substance (positive control):
- yes
- Remarks:
- DCP
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- -- Incolulum control:
The respiration rates of the two controls did not differ by more than 15 %.
-- Reference Item
The 3-hour EC 50 of the reference item 3,5-Dichlorophenol was determined to be 5.0 mg/L and is thus in the range of 5 to 30 mg/L for the used activated sludge batch.
-- Dissolved Oxygen
The concentration of dissolved oxygen did not drop below 2.5 mg 02/L during the incubation period, and just before the measurements of the respiration rates the oxygen concentrations were at least 6.9 mg 02/L.
--Determination of the Inhibitory Effects:
The 3-hour EC 50 of the reference item was calculated by Probit analysis. The 3-hour EC 20 and EC 50 of the test item could not be calculated due to the low toxicity of the test item to micro organisms under the tested conditions. - Results with reference substance (positive control):
- The 3-hour EC 50 of the reference item 3,5-Dichlorophenol was determined to be 13.5 mg/L and is thus in the range of 5 to 30 mg/L for the used activated sludge batch.
- Validity criteria fulfilled:
- yes
Reference
Study design
The influence of the test item on the activity of activated sludge was
evaluated by measuring the respiration rate under defined conditions.
The respiration rate (oxygen consumption) of an aerobic activated sludge
fed with a standard amount of synthetic sewage was measured in the
presence of various concentrations (10, 32, 100, 320 and 1000 mg/L) of
the test item after an incubation period of 3 hours. This study was
conducted according to Good Laboratory Practice (GLP) and the method
applied followed the OECD Guideline for Testing of Chemicals, Section 2,
No.209: "Activated Sludge, Respiration Inhibition Test", adopted April
04, 1984.
Results
In comparison to the inoculum controls, the respiration rate of the activated sludge was inhibited by -5.6% and 7.4% at all tested concentrations up to nominal 1000 mg/L. Higher concentrations were not tested. Based on measured inhibition rates, the 3 hour EC20 and EC50 values of the test item could not be quantified as the inhibition at the highest nominal concentration of 1000 mg/L was less than 20%. However, the 3 -hour EC20 and EC50 are clearly higher than 1000 mg/L.
Conclusion
The 3 -hour EC50 of the test material was higher than 1000 mg/L under the present test conditions.
Description of key information
There is one microorganism study available for TRIS HCl. The study was conducted according to OECD guideline 209 and GLP (6.1.7 -1). Activated sludge of a predominantly domestic sewage was exposed to TRIS HCl for 3 hours, at nominal test concentrations of 10, 32, 100, 320 and 1000 mg/L. In comparison to the inoculum controls the respiration rate of the activated sludge was not or only slightly inhibited by -5.6 % to 7.4 % at all tested concentrations up to 1000 mg/L. The EC10 and EC50 values for the test substance are therefore greater than 1000 mg/L, the highest concentration tested.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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