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EC number: 269-119-5 | CAS number: 68187-67-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08/04/1986 to 22/04/1986
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- yes
- Remarks:
- A multi dose LD50 was not performed
- GLP compliance:
- yes
- Test type:
- standard acute method
Test material
- Reference substance name:
- Amines, C12-14-tert-alkyl, isooctyl mono phosphates
- IUPAC Name:
- Amines, C12-14-tert-alkyl, isooctyl mono phosphates
- Test material form:
- liquid
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Young adult male and female albino New Zealand rabbits obtained from Clerco Research Farm, CincinNati, OH, USA
- Housing: The animals were housed individually in stainless steel cages with wire mesh floors.
- Diet (e.g. ad libitum): Purina Certified Rabbit Chow 5322 was fed throughout the study.
- Water (e.g. ad libitum): Free access to filtered tap water was provided through an automatic watering system.
- Acclimation period: 7 days.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 67 ± 5 F
- Humidity (%): 30 - 70%
- Ventilation: The animal room was well ventilated and air conditioned
- Photoperiod (hrs dark / hrs light): lighting was controlled by automatic timers to give twelve hours continuous light (06:00 to 18:00) and twelve hours darkness.
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
Approximately 24 hours before treatment the dorsal and lateral trunk (approximately 10% of the body surface area) of each animal were clipped free of hair.
On the day of treatment bodyweights were recorded and doses calculated. An impervious binder consistng of plastic wrap and adhesive tape was applied around each animal's trunk and a measured volume of the test article was introduced under the binder and spread over the application site. The entire trunk of each animal was then wrapped with additional adhesive tape and masking tape.
After a 24 Hour exposure period each binder was removed and the test site of each animal was wiped with gauze sponges moistened with propylene glycol to remove remaining test item. - Duration of exposure:
- 24 hours
- Doses:
- 2000mg/kg. A multi dose LD50 was not performed
- No. of animals per sex per dose:
- 5 males and 5 females
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing:
The animals were observed frequently on the day of treatment for deaths or overt signs of toxicity and subsequently once daily for fourteen days.
Individual bodyweights were recorded prior to application of the test item on Day 0 and on Days 7 and 14.
- Necropsy of survivors performed: yes
At the end of the study the animals were killed by intravenous injection of barbiturate and exanguinated prior to necropsy. Animals found dead during the study were necropsied as soon as possible after death. All animals were subjected to gross necropsy. This consisted of an external examination and opening of the abdominal and thoracic cavities. The appearance of any macroscopic abnormalities was recorded. No tissues were retained.
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- approximate LD50
- Effect level:
- ca. 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- One female was found dead on Days 2 and 6. Two males and one females were found dead on Day 7.
- Clinical signs:
- other: Observations noted during the study were erythema, edema, black / brown disclouration, atonia, eschar formation, fissures and or disquamation of the test sites, muscle tremors, few / no stools, loose stools, no urine, food appeared undisturbed, emaciation
- Gross pathology:
- Necropsy of animals found dead revealed treated skins that were discolored red, black, or red black, thickened, crusted and / or stiff; black disclourations on the mucosa of stomachs; dark contents of cecums; tan disclourations on the surface of the left lateral lobe of one liver; fluid content of small intestine; and / or urinary bladder distended with gelatinous amber fluid.
Necropsy of surviving animals revealed linear abrasions, crusting sloughing epidermis, ulcerative dermatitis, thickened areas with hyperemic borders, multiple scabs, and / or underlying granulation tissue on the treated area of the back
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- Under the conditions of this study, the acute dermal LD50 of the test material was considered to be approximately 2000 mg/kg body weight.
- Executive summary:
Method
The test material was applied topically to five male and five female New Zealand White Rabbits at a dose level of 2000 mg/kg body weight for a period of 24 hours. The animals were observed for pharmacotoxic signs and mortality for 14 days following the day of treatment.
Mortality
Two males and three females died following treatment. Deaths ocurred on Days 2, 6, and 7 after test material application.
Clinical signs and Bodweight
Observation noted during the study were erythema, edema, black / brown disclouration, atonia, eschar formation, fissures and or disquamation of the test sites, muscle tremors, few / no stools, loose stools, no urine, food appeared undisturbed, emaciation, lethargy, salivation, ataxia, red stained fur around test sites poor coat quality.
All surviving animals except one gained weight and one female had no overall weight gain
Necropsy
Necropsy of animals found dead revealed treated skins that were discolored red, black, or red black, thickened, crusted and / or stiff; black disclourations on the mucosa of stomachs; dark contents of cecums; tan disclourations on the surface of the left lateral lobe of one liver; fluid content of small intestine; and / or urinary bladder distended with gelatinous amber fluid. Necropsy of surviving animals revealed linear abrasions, crusting sloughing epidermis, ulcerative dermatitis, thickened areas with hyperemic borders, multiple scabs, and / or underlying granulation tissue on the treated area of the back.
Conclusion
Under the conditions of this study, the acute dermal LD50 of the test material was considered to be approximately 2000 mg/kg body weight.
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